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rdf:type
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Description
| - IRS-2 from the hard tick Ixodes ricinus belongs into the serpin family of protease inhibitors. It is produced in the tick salivary glands, and its anti-inflammatory activity suggests a role in the parasitehost interaction. Recombinant IRS-2 prepared by heterologous expression in bacterial system was crystallized and crystals diffracted to resolution 1.8 Å. IRS-2 was cleaved during crystallization by contaminating proteases. This processing of IRS-2 mimicked the specific cleavage of serpin by its target protease and resulted in a more stable R conformation, which produced well-diffracting crystals. Activity profiling with specific substrates and inhibitors demonstrated traces of serine and cysteine proteases in the protein stock solution.
- IRS-2 from the hard tick Ixodes ricinus belongs into the serpin family of protease inhibitors. It is produced in the tick salivary glands, and its anti-inflammatory activity suggests a role in the parasitehost interaction. Recombinant IRS-2 prepared by heterologous expression in bacterial system was crystallized and crystals diffracted to resolution 1.8 Å. IRS-2 was cleaved during crystallization by contaminating proteases. This processing of IRS-2 mimicked the specific cleavage of serpin by its target protease and resulted in a more stable R conformation, which produced well-diffracting crystals. Activity profiling with specific substrates and inhibitors demonstrated traces of serine and cysteine proteases in the protein stock solution. (en)
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Title
| - Crystallization and diffraction analysis of the serpin IRS-2 from the hard tick Ixodes ricinus
- Crystallization and diffraction analysis of the serpin IRS-2 from the hard tick Ixodes ricinus (en)
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skos:prefLabel
| - Crystallization and diffraction analysis of the serpin IRS-2 from the hard tick Ixodes ricinus
- Crystallization and diffraction analysis of the serpin IRS-2 from the hard tick Ixodes ricinus (en)
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skos:notation
| - RIV/68378050:_____/10:00349395!RIV11-GA0-68378050
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http://linked.open...avai/riv/aktivita
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http://linked.open...avai/riv/aktivity
| - P(GAP207/10/2183), P(LC06009), Z(AV0Z40550506), Z(AV0Z50520514), Z(AV0Z60220518)
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http://linked.open...iv/cisloPeriodika
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http://linked.open...vai/riv/dodaniDat
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http://linked.open...aciTvurceVysledku
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http://linked.open.../riv/druhVysledku
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http://linked.open...iv/duvernostUdaju
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http://linked.open...titaPredkladatele
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http://linked.open...dnocenehoVysledku
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http://linked.open...ai/riv/idVysledku
| - RIV/68378050:_____/10:00349395
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http://linked.open...riv/jazykVysledku
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http://linked.open.../riv/klicovaSlova
| - protease inhibitor; serpin; tick; proteolysis (en)
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http://linked.open.../riv/klicoveSlovo
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http://linked.open...odStatuVydavatele
| - GB - Spojené království Velké Británie a Severního Irska
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http://linked.open...ontrolniKodProRIV
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http://linked.open...i/riv/nazevZdroje
| - Acta Crystallographica Section F
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http://linked.open...in/vavai/riv/obor
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http://linked.open...ichTvurcuVysledku
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http://linked.open...cetTvurcuVysledku
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http://linked.open...vavai/riv/projekt
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http://linked.open...UplatneniVysledku
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http://linked.open...v/svazekPeriodika
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http://linked.open...iv/tvurceVysledku
| - Brynda, Jiří
- Chmelař, Jindřich
- Kovářová, Zuzana
- Mareš, Michael
- Řezáčová, Pavlína
- Šanda, Miloslav
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http://linked.open...ain/vavai/riv/wos
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http://linked.open...n/vavai/riv/zamer
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issn
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number of pages
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