About: Transient expression of human papillomavirus type 16 virus-like particles in tobacco and tomato using a tobacco rattle virus expression vector

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An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	The major capsid protein L1 of human papillomavirus type 16 (HPV16) was transiently expressed in tobacco (Nicotiana benthamiana and Nicotiana tabacum) and tomato (Lycopersicon esculentum) leaves using Agrobacterium tumefaciens. The expression vector pTV00 was derived from tobacco rattle virus (TRV). The highest L1 expression 15 mg g-1 was achieved when the coding sequence of L1 was optimized for expression in humans that caused an increase of the GC content from 38.2 % in wild type HPV16 to 64.1 % in optimized sequence. L1 monomers readily self-assembled into capsomeres and further into virus like particles (VLPs). Immunological characterization and electron microscopy showed that 89 % of L1 retained this structure also in extracts prepared from freeze-dried leaves. Plant expressed L1 was highly immunogenic without any additional adjuvant as vaccinated mice developed strong humoral and cellular immune response, comparable to that elicited by purified VLPs derived from insect cells. The major capsid protein L1 of human papillomavirus type 16 (HPV16) was transiently expressed in tobacco (Nicotiana benthamiana and Nicotiana tabacum) and tomato (Lycopersicon esculentum) leaves using Agrobacterium tumefaciens. The expression vector pTV00 was derived from tobacco rattle virus (TRV). The highest L1 expression 15 mg g-1 was achieved when the coding sequence of L1 was optimized for expression in humans that caused an increase of the GC content from 38.2 % in wild type HPV16 to 64.1 % in optimized sequence. L1 monomers readily self-assembled into capsomeres and further into virus like particles (VLPs). Immunological characterization and electron microscopy showed that 89 % of L1 retained this structure also in extracts prepared from freeze-dried leaves. Plant expressed L1 was highly immunogenic without any additional adjuvant as vaccinated mice developed strong humoral and cellular immune response, comparable to that elicited by purified VLPs derived from insect cells. (en)
Title	Transient expression of human papillomavirus type 16 virus-like particles in tobacco and tomato using a tobacco rattle virus expression vector Transient expression of human papillomavirus type 16 virus-like particles in tobacco and tomato using a tobacco rattle virus expression vector (en)
skos:prefLabel	Transient expression of human papillomavirus type 16 virus-like particles in tobacco and tomato using a tobacco rattle virus expression vector Transient expression of human papillomavirus type 16 virus-like particles in tobacco and tomato using a tobacco rattle virus expression vector (en)
skos:notation	RIV/68378050:_____/10:00345055!RIV11-GA0-68378050
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(1M0505), P(GA521/01/1418), P(GA521/04/0971), P(IAA6038201), P(IBS5038304), Z(AV0Z50380511), Z(AV0Z50520514)
http://linked.open...iv/cisloPeriodika	3
http://linked.open...vai/riv/dodaniDat	2011
http://linked.open...aciTvurceVysledku	Angelisová, Pavla
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Ústav molekulární genetiky AV ČR, v. v. i.
http://linked.open...dnocenehoVysledku	293327
http://linked.open...ai/riv/idVysledku	RIV/68378050:_____/10:00345055
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	ELISA; Lycopersicon esculentum; Nicotiana benthamiana (en)
http://linked.open.../riv/klicoveSlovo	ELISA Nicotiana benthamiana Lycopersicon esculentum
http://linked.open...odStatuVydavatele	NL - Nizozemsko
http://linked.open...ontrolniKodProRIV	[17C93F7D7EDD]
http://linked.open...i/riv/nazevZdroje	Biologia Plantarum
http://linked.open...in/vavai/riv/obor	EB
http://linked.open...ichTvurcuVysledku	1 (xsd:int)
http://linked.open...cetTvurcuVysledku	7 (xsd:int)
http://linked.open...vavai/riv/projekt	Center of targeted therapeutic drugs Moss (Physcomitrella Patents) as a crop plant The role of plant systemic acquired resistance (SAR) in response to genotoxic stress Inducible DNA repair in Arabidopsis thaliana Plant produced recombinant antibodies
http://linked.open...UplatneniVysledku	2010
http://linked.open...v/svazekPeriodika	54
http://linked.open...iv/tvurceVysledku	Angelisová, Pavla Müller, M. Angelis, Karel Velemínský, Jiří Šmídková, Markéta Piuko, K. Thönes, N.
http://linked.open...ain/vavai/riv/wos	000279366200008
http://linked.open...n/vavai/riv/zamer	Mechanismy regulace růstu a vývoje rostlin na úrovni buněk, orgánů a celých organismů: fyziologické, genetické a molekulárně biologické základy Molekulárně genetické a buněčné základy klíčových biologických procesů: genová exprese, onkogeneze, replikace virů, imunita a vývoj organismů
issn	0006-3134
number of pages	10 (xsd:int)

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