About: Optimalization of immunogold labeling on thin resin sections after cryofixation

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Various resins (Lowicryl, LR White, LR Gold, Unicryl) used for embedding were compared for their ability to reveal antigens for immunogold labelling on their surface. Their surfaces after cutting with a diamond knife were observed using atomic force microscopy, and results statistically analyzed. The LR White resin in combination with HPF/FS was shown to be able to be successfully used for fine ultrastructural immunocytochemistry. Methods were presented allowing detection of clustering or colocalization of antigens/gold particles. A complete set of tools is now available for reliable detection of gold particles in EM images, their statistical evaluation and production of convenient outputs for publication of results. The plug-ins are useful addition to image analysis software accompanying modern CCD cameras for electron microscopes. Various resins (Lowicryl, LR White, LR Gold, Unicryl) used for embedding were compared for their ability to reveal antigens for immunogold labelling on their surface. Their surfaces after cutting with a diamond knife were observed using atomic force microscopy, and results statistically analyzed. The LR White resin in combination with HPF/FS was shown to be able to be successfully used for fine ultrastructural immunocytochemistry. Methods were presented allowing detection of clustering or colocalization of antigens/gold particles. A complete set of tools is now available for reliable detection of gold particles in EM images, their statistical evaluation and production of convenient outputs for publication of results. The plug-ins are useful addition to image analysis software accompanying modern CCD cameras for electron microscopes. (en)
Title	Optimalization of immunogold labeling on thin resin sections after cryofixation Optimalization of immunogold labeling on thin resin sections after cryofixation (en)
skos:prefLabel	Optimalization of immunogold labeling on thin resin sections after cryofixation Optimalization of immunogold labeling on thin resin sections after cryofixation (en)
skos:notation	RIV/68378050:_____/09:00336266!RIV10-MSM-68378050
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(2B06063), P(GD204/09/H084), P(KAN200520704), P(LC545), Z(AV0Z40400503), Z(AV0Z50110509), Z(AV0Z50520514)
http://linked.open...vai/riv/dodaniDat	2010
http://linked.open...aciTvurceVysledku	Sobol, Margaryta Philimonenko, Anatolij Hozák, Pavel Philimonenko, Vlada
http://linked.open.../riv/druhVysledku	D - Článek ve sborníku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Ústav molekulární genetiky AV ČR, v. v. i.
http://linked.open...dnocenehoVysledku	331969
http://linked.open...ai/riv/idVysledku	RIV/68378050:_____/09:00336266
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	cell ultrastructure; high pressure freezing; freeze substitutions; resins; immunocytochemistry (en)
http://linked.open.../riv/klicoveSlovo	cell ultrastructure freeze substitutions high pressure freezing immunocytochemistry resins
http://linked.open...ontrolniKodProRIV	[FCF0508FCCAE]
http://linked.open...v/mistoKonaniAkce	Graz
http://linked.open...i/riv/mistoVydani	Graz
http://linked.open...i/riv/nazevZdroje	MC 2009 - Microscopy Conference: First Joint Meeting of Dreiländertagung and Multinational Conference on Microscopy
http://linked.open...in/vavai/riv/obor	EB
http://linked.open...ichTvurcuVysledku	4 (xsd:int)
http://linked.open...cetTvurcuVysledku	8 (xsd:int)
http://linked.open...vavai/riv/projekt	Structure and irregularities of the nucleoskeleton: identification and diagnostics. Functional Organisation of the Cell Molecular biology of a differentiating cell New nanoparticles for ultrastructural diagnostics
http://linked.open...UplatneniVysledku	2009
http://linked.open...iv/tvurceVysledku	Hozák, Pavel Janáček, Jiří Kubínová, Lucie Philimonenko, Anatolij Sobol, Margaryta Janda, Pavel Vyhnal, Aleš Philimonenko, Vlada
http://linked.open...vavai/riv/typAkce	WRD - Světová
http://linked.open.../riv/zahajeniAkce	2009-08-30 (xsd:date)
http://linked.open...n/vavai/riv/zamer	Struktura, reaktivita a dynamika molekulárních a biomolekulárních systémů: teorie, experiment, aplikace Výzkum molekulárních a buněčných základů fyziologických a patofyziologických procesů s cílem objasnit mechanismy vzniku závažných onemocnění člověka Molekulárně genetické a buněčné základy klíčových biologických procesů: genová exprese, onkogeneze, replikace virů, imunita a vývoj organismů
number of pages	2 (xsd:int)
http://purl.org/ne...btex#hasPublisher	Verlag der Technischen Universität
https://schema.org/isbn	978-3-85125-062-6

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