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| rdf:type
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| Description
| - Proteins from different cellular compartments were labeled and separated by size exclusion chromatography into 20 fractions. The elution profiles were compiled to color maps across the size separation range (670-10kDa). A new solid phase designed for processing in microwell plates was developed to handle the large number of samples. Antibodies were bound to protein G-coupled microspheres surface-labeled with 300 combinations of four fluorescent dyes. Fluorescence from particle color codes and the protein label was measured by high speed flow cytometry. Cytoplasmic protein kinases, membrane proteins, cyclin-dependent kinases (cdks), cyclins and cdk-inhibitors, were analyzed; the results were compatible with their occurrence in complexes that vary with the cell cycle phase and subcellular localization. A two-dimensional platform extends the utility of antibody array analysis to studies of protein complexes.
- Proteins from different cellular compartments were labeled and separated by size exclusion chromatography into 20 fractions. The elution profiles were compiled to color maps across the size separation range (670-10kDa). A new solid phase designed for processing in microwell plates was developed to handle the large number of samples. Antibodies were bound to protein G-coupled microspheres surface-labeled with 300 combinations of four fluorescent dyes. Fluorescence from particle color codes and the protein label was measured by high speed flow cytometry. Cytoplasmic protein kinases, membrane proteins, cyclin-dependent kinases (cdks), cyclins and cdk-inhibitors, were analyzed; the results were compatible with their occurrence in complexes that vary with the cell cycle phase and subcellular localization. A two-dimensional platform extends the utility of antibody array analysis to studies of protein complexes. (en)
- Proteiny různých velikostí byly fluorescenčně označeny a separovány gelovou filtrací do 20 frakcí. Eluční profily byly uspořádány do barevných map v celém separačním rozsahu (670-10 kDa). Byl vyvinut nový typ nosiče vhodný pro zpracování velkého počtu vzorků v mikrodestičkách. Protilátky byly navázány na mikrokuličky s proteinem G značené 300 kombinacemi 4 fluoroforů a detekovány vysokorychlostní průtokovou cytometrií. Byly analyzovány proteinkinasy, membránové proteiny, cykliny a cyklin-dependentní kinasy. Výsledky odpovídaly známým komplexům těchto proteinů a jejich subcelulární lokalizaci v závislosti na buněčném cykly. tato dvojrozměrná platforma je vhodná jako nová metoda pro studium proteinových komplexů. (cs)
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| Title
| - Antibody array analysis with label-based detection and resolution of protein size
- Analýza založená na mnohočetném protilátkovém souboru, detekci značením a rozlišení velikosti proteinů (cs)
- Antibody array analysis with label-based detection and resolution of protein size (en)
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| skos:prefLabel
| - Antibody array analysis with label-based detection and resolution of protein size
- Analýza založená na mnohočetném protilátkovém souboru, detekci značením a rozlišení velikosti proteinů (cs)
- Antibody array analysis with label-based detection and resolution of protein size (en)
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| skos:notation
| - RIV/68378050:_____/09:00318349!RIV09-AV0-68378050
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| http://linked.open...avai/riv/aktivita
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| http://linked.open...avai/riv/aktivity
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| http://linked.open...iv/cisloPeriodika
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| http://linked.open...vai/riv/dodaniDat
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| http://linked.open...aciTvurceVysledku
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| http://linked.open.../riv/druhVysledku
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| http://linked.open...iv/duvernostUdaju
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| http://linked.open...titaPredkladatele
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| http://linked.open...dnocenehoVysledku
| |
| http://linked.open...ai/riv/idVysledku
| - RIV/68378050:_____/09:00318349
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| http://linked.open...riv/jazykVysledku
| |
| http://linked.open.../riv/klicovaSlova
| - antibody array; proteomics; cell signalling (en)
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| http://linked.open.../riv/klicoveSlovo
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| http://linked.open...odStatuVydavatele
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| http://linked.open...ontrolniKodProRIV
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| http://linked.open...i/riv/nazevZdroje
| - Molecular and Cellular Proteomics
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| http://linked.open...in/vavai/riv/obor
| |
| http://linked.open...ichTvurcuVysledku
| |
| http://linked.open...cetTvurcuVysledku
| |
| http://linked.open...UplatneniVysledku
| |
| http://linked.open...v/svazekPeriodika
| |
| http://linked.open...iv/tvurceVysledku
| - Frey, T.
- Hořejší, Václav
- Wu, W.
- Aasheim, H. C.
- Boretti, E.
- De la Rosa Carrillo, D.
- Lund-Jaohnsen, F.
- Slaastad, H. S.
- Tjonnfjord, G. E.
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| http://linked.open...n/vavai/riv/zamer
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| issn
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| number of pages
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