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| rdf:type
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| Description
| - Differentiation of hematopoietic stem cells and progenitors into various lineages is controlled by a complex array of extrinsic and intrinsic factors. Here we have identified, cloned and characterized chicken thrombopoietin, the first non-mammalian Tpo and its receptor c-Mpl. Chicken Tpo is expressed in the liver and spleen whilst c-mpl is expressed in the bone marrow, lung and spleen. In vitro experiments with sorted multipotent bone marrow-derived progenitors demonstrate the committing role of Tpo for the thrombocytic lineage. In cooperation with stem cell factor Tpo also plays a role in self-renewal of multipotent progenitors. Intravenous application of recombinant chicken Tpo or overexpresion of Tpo and c-mpl via retroviral infection lead to erythroblastosis and thromboblastosis in animals. In this work we have introduced the experimental model of the chicken bi-potent thrombo-/erythropoietic progenitor that can be useful for identifying key regulators of cell fate determination.
- Differentiation of hematopoietic stem cells and progenitors into various lineages is controlled by a complex array of extrinsic and intrinsic factors. Here we have identified, cloned and characterized chicken thrombopoietin, the first non-mammalian Tpo and its receptor c-Mpl. Chicken Tpo is expressed in the liver and spleen whilst c-mpl is expressed in the bone marrow, lung and spleen. In vitro experiments with sorted multipotent bone marrow-derived progenitors demonstrate the committing role of Tpo for the thrombocytic lineage. In cooperation with stem cell factor Tpo also plays a role in self-renewal of multipotent progenitors. Intravenous application of recombinant chicken Tpo or overexpresion of Tpo and c-mpl via retroviral infection lead to erythroblastosis and thromboblastosis in animals. In this work we have introduced the experimental model of the chicken bi-potent thrombo-/erythropoietic progenitor that can be useful for identifying key regulators of cell fate determination. (en)
- Diferenciace hematopoetických kmenových buněk a progenitorů do různých linií je kontrolována řadou vnějších a vnitřních faktorů. Identifikovali, klonovali a charakterizovali jsme kuřecí trombopoetin, první nesavčí Tpo a jeho receptor c-Mpl. Kuřecí Tpo se exprimuje v játrech a slezině, zatímco c-mpl se exprimuje v kostní dřeni, plicích a slezině. Pokusy in vitro ukazují, že Tpo směruje multipotentními progenitory z kostní dřeně do trombocytární linie. Tpo společně s SCF hraje svou roli v sebeonově multipotemtních progenitorů. Nitrožilní aplikace rekombinantního kuřecího Tpo nebo nadexprese Tpo a c-mpl pomocí retrovirů vede k erytroblastose a tromboblastose u zvířat. V této práci jsme zavedli model kuřecích bipotentních trombo/erytropoetických progenitorů které mohou být užitečné pro identifikaci klíčových regulátorů buněčného osudu. (cs)
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| Title
| - Impact of chicken thrombopoietin and its receptor c-Mpl on hematopoietic cell development
- Úloha kuřecího trombopoetinu a jeho receptoru c-Mpl ve vývoji hematopoetických buněk (cs)
- Impact of chicken thrombopoietin and its receptor c-Mpl on hematopoietic cell development (en)
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| skos:prefLabel
| - Impact of chicken thrombopoietin and its receptor c-Mpl on hematopoietic cell development
- Úloha kuřecího trombopoetinu a jeho receptoru c-Mpl ve vývoji hematopoetických buněk (cs)
- Impact of chicken thrombopoietin and its receptor c-Mpl on hematopoietic cell development (en)
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| skos:notation
| - RIV/68378050:_____/08:00318451!RIV09-AV0-68378050
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| http://linked.open...avai/riv/aktivita
| |
| http://linked.open...avai/riv/aktivity
| - P(GA204/06/1728), P(LC06061), P(LC06077), Z(AV0Z50520514)
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| http://linked.open...iv/cisloPeriodika
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| http://linked.open...vai/riv/dodaniDat
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| http://linked.open...aciTvurceVysledku
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| http://linked.open.../riv/druhVysledku
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| http://linked.open...iv/duvernostUdaju
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| http://linked.open...titaPredkladatele
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| http://linked.open...dnocenehoVysledku
| |
| http://linked.open...ai/riv/idVysledku
| - RIV/68378050:_____/08:00318451
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| http://linked.open...riv/jazykVysledku
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| http://linked.open.../riv/klicovaSlova
| - Tpo; thrombocytes; cell fate determination (en)
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| http://linked.open.../riv/klicoveSlovo
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| http://linked.open...odStatuVydavatele
| - US - Spojené státy americké
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| http://linked.open...ontrolniKodProRIV
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| http://linked.open...i/riv/nazevZdroje
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| http://linked.open...in/vavai/riv/obor
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| http://linked.open...ichTvurcuVysledku
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| http://linked.open...cetTvurcuVysledku
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| http://linked.open...vavai/riv/projekt
| |
| http://linked.open...UplatneniVysledku
| |
| http://linked.open...v/svazekPeriodika
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| http://linked.open...iv/tvurceVysledku
| - Bartůněk, Petr
- Dvořák, Michal
- Králová, Jarmila
- Karafiát, Vít
- Pajer, Petr
- Dvořáková, Marta
- Bartůňková, Jana
- Zenke, M.
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| http://linked.open...n/vavai/riv/zamer
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| issn
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| number of pages
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