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Description
  • The epigenetic status of human spermatozoa is difficult to analyse. The method of interspecies fertilization can be used for different purposes. The aim of our work was to adopt this approach for the detailed analysis of epigenetic status of human spermatozoa injected into mouse oocytes. Our results show that human spermatozoa injected into mouse oocytes fully respond to oocyte cytoplasmic factors and form analysable pronuclei. The labeling of zygotes showed that as in other species, the paternal chromatin is extensively epigenetically remodeled. The interspecies ICSI may be a powerful tool for the analysis of sperm epigenetic status even with a very low number of spermatozoa available.
  • The epigenetic status of human spermatozoa is difficult to analyse. The method of interspecies fertilization can be used for different purposes. The aim of our work was to adopt this approach for the detailed analysis of epigenetic status of human spermatozoa injected into mouse oocytes. Our results show that human spermatozoa injected into mouse oocytes fully respond to oocyte cytoplasmic factors and form analysable pronuclei. The labeling of zygotes showed that as in other species, the paternal chromatin is extensively epigenetically remodeled. The interspecies ICSI may be a powerful tool for the analysis of sperm epigenetic status even with a very low number of spermatozoa available. (en)
  • Epigenetický charakter lidských spermií je obtížné analyzovat. Metoda mezidruhové fertilizace může být použita pro různé účely. Cílem naší práce bylo aplikovat tento přístup na podrobnou analýzu epigenetického charakteru lidských spermií, naočkovaných do lidských oocytů. Naše výsledky ukazují, že lidské spermie, naočkované do myších oocytů, plně reagují na cytoplasmické faktory oocytů a tvoří analyzovatelná projádra. Značení zygot ukázalo, že - podobně jako u jiných druhů – parentální chromatin je rozsáhle epigeneticky remodelován. Mezidruhové ICSI mohou být efektním prostředkem pro analýzu epigenetického charakteru spermií i při dostupnosti nízkého počtu spermií. (cs)
Title
  • Epigenetic analysis of human spermatozoa after their injection into ovulated mouse oocytes
  • Epigenetic analysis of human spermatozoa after their injection into ovulated mouse oocytes (en)
  • Epigenetická analýza lidských spermií po jejich naočkování do myších oocytů (cs)
skos:prefLabel
  • Epigenetic analysis of human spermatozoa after their injection into ovulated mouse oocytes
  • Epigenetic analysis of human spermatozoa after their injection into ovulated mouse oocytes (en)
  • Epigenetická analýza lidských spermií po jejich naočkování do myších oocytů (cs)
skos:notation
  • RIV/68378050:_____/08:00318360!RIV09-AV0-68378050
http://linked.open...avai/riv/aktivita
http://linked.open...avai/riv/aktivity
  • P(GD204/05/H023), Z(AV0Z50520514)
http://linked.open...iv/cisloPeriodika
  • 3
http://linked.open...vai/riv/dodaniDat
http://linked.open...aciTvurceVysledku
http://linked.open.../riv/druhVysledku
http://linked.open...iv/duvernostUdaju
http://linked.open...titaPredkladatele
http://linked.open...dnocenehoVysledku
  • 366393
http://linked.open...ai/riv/idVysledku
  • RIV/68378050:_____/08:00318360
http://linked.open...riv/jazykVysledku
http://linked.open.../riv/klicovaSlova
  • spermatozoa; epigenetics; chromatin; acetylation; methylation (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...odStatuVydavatele
  • GB - Spojené království Velké Británie a Severního Irska
http://linked.open...ontrolniKodProRIV
  • [2F5235188145]
http://linked.open...i/riv/nazevZdroje
  • Human Reproduction
http://linked.open...in/vavai/riv/obor
http://linked.open...ichTvurcuVysledku
http://linked.open...cetTvurcuVysledku
http://linked.open...vavai/riv/projekt
http://linked.open...UplatneniVysledku
http://linked.open...v/svazekPeriodika
  • 23
http://linked.open...iv/tvurceVysledku
  • Fulka, J.
  • Fulka, Helena
  • Barnetová, I.
  • Moško, T.
http://linked.open...ain/vavai/riv/wos
  • 000253746900022
http://linked.open...n/vavai/riv/zamer
issn
  • 1355-4786
number of pages
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