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| rdf:type
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| Description
| - Signální dráha proteinu Wnt ovlivňuje proliferaci a udržování hemapoetických buněk, kdežto cytotoxický ligand Apo2L/TRAIL navozuje apoptózu různých transformovaných buněk. Kokultivací lidských pre-B leukemických buněčných linií KM3 nebo REH spolu s krysími embryonálními fibroblasty, jež produkují proteiny Wnt1 nebo Wnt3a, byla úspěšně potlačena Apo2L/TRAIL způsobená apoptóza leukemických buněk. Tato suprese se děje na úrovni ranných stadií Apo2L/TRAIL indukované apoptotické kaskády, přičemž samotné spuštění signalizace proteinu Wnt v lidských leukemických buňkách nevede k dostatečné ochraně proti apoptóze. Inhibice MEK1/MEK2 nebo NFkB signální dráhy v pre-B liniích KM3 a REH zrušila antiapoptotický efekt Wnt produkujích fibroblastů. Tyto výsledky naznačují, že parakrinní signály spojené s dráhou proteinu Wnt mohou být důležité pro přežívání pre-B leukemických buněk. (cs)
- The Wnt signaling enhances proliferation and maintenance of hematopoietic cells. In contrary, the cytotoxic ligand Apo2L/TRAIL induces apoptosis of transformed cells. We observed that pre-cultivation of pre-B leukemia cell lines (KM3, REH) on Wnt1- or Wnt3a-expressing Rat2 fibroblasts efficiently suppressed their Apo2L/TRAIL -induced apoptosis. This suppression occurs at the early stages of the Apo2L/TRAIL apoptotic signaling and is only partially dependent on the Wnt pathway alone. Anti-apoptotic Rat2Wnt1 signaling was significantly hampered by the inhibition of MEK1/MEK2 or NFkB pathways in pre-B acceptor cells. Our results implicate the existence of an additional signaling pathway in Wnt1-transfected fibroblasts that in conjunction with the Wnt-triggered cascade of cellular events suppresses Apo2L/TRAIL-induced apoptosis of pre-B cells. Such composed antiapoptotic signaling could contribute to pre-B leukemias survival.
- The Wnt signaling enhances proliferation and maintenance of hematopoietic cells. In contrary, the cytotoxic ligand Apo2L/TRAIL induces apoptosis of transformed cells. We observed that pre-cultivation of pre-B leukemia cell lines (KM3, REH) on Wnt1- or Wnt3a-expressing Rat2 fibroblasts efficiently suppressed their Apo2L/TRAIL -induced apoptosis. This suppression occurs at the early stages of the Apo2L/TRAIL apoptotic signaling and is only partially dependent on the Wnt pathway alone. Anti-apoptotic Rat2Wnt1 signaling was significantly hampered by the inhibition of MEK1/MEK2 or NFkB pathways in pre-B acceptor cells. Our results implicate the existence of an additional signaling pathway in Wnt1-transfected fibroblasts that in conjunction with the Wnt-triggered cascade of cellular events suppresses Apo2L/TRAIL-induced apoptosis of pre-B cells. Such composed antiapoptotic signaling could contribute to pre-B leukemias survival. (en)
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| Title
| - Wnt-expressing rat embryonic fibroblasts suppress Apo2L/TRAIL-induced apoptosis of human leukemia cells
- Wnt produkující krysí embryonální fibroblasty potlačují Apo2L/TRAIL indukovanou apoptózu lidských leukemických buněk (cs)
- Wnt-expressing rat embryonic fibroblasts suppress Apo2L/TRAIL-induced apoptosis of human leukemia cells (en)
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| skos:prefLabel
| - Wnt-expressing rat embryonic fibroblasts suppress Apo2L/TRAIL-induced apoptosis of human leukemia cells
- Wnt produkující krysí embryonální fibroblasty potlačují Apo2L/TRAIL indukovanou apoptózu lidských leukemických buněk (cs)
- Wnt-expressing rat embryonic fibroblasts suppress Apo2L/TRAIL-induced apoptosis of human leukemia cells (en)
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| skos:notation
| - RIV/68378050:_____/08:00306874!RIV09-AV0-68378050
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| http://linked.open...avai/riv/aktivita
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| http://linked.open...avai/riv/aktivity
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| http://linked.open...iv/cisloPeriodika
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| http://linked.open...vai/riv/dodaniDat
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| http://linked.open...aciTvurceVysledku
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| http://linked.open.../riv/druhVysledku
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| http://linked.open...iv/duvernostUdaju
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| http://linked.open...titaPredkladatele
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| http://linked.open...dnocenehoVysledku
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| http://linked.open...ai/riv/idVysledku
| - RIV/68378050:_____/08:00306874
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| http://linked.open...riv/jazykVysledku
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| http://linked.open.../riv/klicovaSlova
| - Wnt; Apo2L/TRAIL; pre-B lymphocyte (en)
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| http://linked.open.../riv/klicoveSlovo
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| http://linked.open...odStatuVydavatele
| - US - Spojené státy americké
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| http://linked.open...ontrolniKodProRIV
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| http://linked.open...i/riv/nazevZdroje
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| http://linked.open...in/vavai/riv/obor
| |
| http://linked.open...ichTvurcuVysledku
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| http://linked.open...cetTvurcuVysledku
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| http://linked.open...UplatneniVysledku
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| http://linked.open...v/svazekPeriodika
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| http://linked.open...iv/tvurceVysledku
| - Anděra, Ladislav
- Valenta, Tomáš
- Doubravská, Lenka
- Kořínek, Vladimír
- Čermák, Lukáš
- Šímová, Šárka
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| http://linked.open...ain/vavai/riv/wos
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| http://linked.open...n/vavai/riv/zamer
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| issn
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| number of pages
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