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Description
| - ASLV family retroviruses contain 5 highly related envelope subgroups (A-E). Susceptibility or resistance of cells to infection by these ASLVs is given by 3 loci in chicken. The tvb gene encodes subgroup B, D, E ASLV receptors. Wild-type TvbS1 receptor confers susceptibility to subgroup B, D, E ASLVs. In this study the genetic defect underlying altered susceptibility of inbred chicken line M to infection by ASLV(B), (D), (E), C125S substitution in tvb-encoded TvbS1 receptor in this line, was identified. We show that this substitution significantly reduces susceptibility of line M cells to infection by ASLV(B) and (D) and virtually eliminates susceptibility to ASLV(E) infection in cultured cells and the incidence and growth of ASV-induced sarcomas in chickens. It also significantly reduces binding affinity of TvbS1 receptor for subgroup B, D, E ASLV envelope glycoproteins. These results are first demonstration of possible role of the Cys-rich domain 3 in the function of Tvb receptors.
- ASLV family retroviruses contain 5 highly related envelope subgroups (A-E). Susceptibility or resistance of cells to infection by these ASLVs is given by 3 loci in chicken. The tvb gene encodes subgroup B, D, E ASLV receptors. Wild-type TvbS1 receptor confers susceptibility to subgroup B, D, E ASLVs. In this study the genetic defect underlying altered susceptibility of inbred chicken line M to infection by ASLV(B), (D), (E), C125S substitution in tvb-encoded TvbS1 receptor in this line, was identified. We show that this substitution significantly reduces susceptibility of line M cells to infection by ASLV(B) and (D) and virtually eliminates susceptibility to ASLV(E) infection in cultured cells and the incidence and growth of ASV-induced sarcomas in chickens. It also significantly reduces binding affinity of TvbS1 receptor for subgroup B, D, E ASLV envelope glycoproteins. These results are first demonstration of possible role of the Cys-rich domain 3 in the function of Tvb receptors. (en)
- Ptačí sarkomové a leukosové viry (ASLV) tvoří pět interferenčních podskupin (A až E) a využívají pro vstup do buněk tří různých receptorů. Viry podskupin B, D a E vstupují do susceptibilních buněk přes receptor Tvb. Identifikovali jsme mutaci v receptoru Tvb, která podmiňuje semiresistentní fenotyp drůbeží inbrední linie M vůči virům podskupiny B a D a téměř úplnou resistenci ke skupině E. Mutace spočívá v záměně cysteinu v posici 125 v cysteinem bohaté doméně 3, která byla doposud pokládána za nedůležitou pro vazbu virových obalových glykoproteinů. Předpokládáme, že při absenci cytosinu 125 dochází k prodloužení receptorové molekuly a navázaný retrovirus je tak příliš daleko od membrány než aby mohl účinně fúsovat s cílovou buňkou. (cs)
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Title
| - A single-amino-acid substitution in the TvbS1 receptor results in decreased susceptibility to infection by avian sarcoma and leukosis virus subgroups B and D and resistance to infection by subgroup E in vitro and in vivo
- A single-amino-acid substitution in the TvbS1 receptor results in decreased susceptibility to infection by avian sarcoma and leukosis virus subgroups B and D and resistance to infection by subgroup E in vitro and in vivo (en)
- Jednoaminokyselinová substituce v receptoru TvbS1 snižuje susceptibilitu k infekci ptačími sarkomovými a leukosovými viry podskupin B a D a podmiňuje resistenci k podskupině E in vitro a in vivo (cs)
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skos:prefLabel
| - A single-amino-acid substitution in the TvbS1 receptor results in decreased susceptibility to infection by avian sarcoma and leukosis virus subgroups B and D and resistance to infection by subgroup E in vitro and in vivo
- A single-amino-acid substitution in the TvbS1 receptor results in decreased susceptibility to infection by avian sarcoma and leukosis virus subgroups B and D and resistance to infection by subgroup E in vitro and in vivo (en)
- Jednoaminokyselinová substituce v receptoru TvbS1 snižuje susceptibilitu k infekci ptačími sarkomovými a leukosovými viry podskupin B a D a podmiňuje resistenci k podskupině E in vitro a in vivo (cs)
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skos:notation
| - RIV/68378050:_____/08:00306812!RIV09-AV0-68378050
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http://linked.open...avai/riv/aktivita
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http://linked.open...avai/riv/aktivity
| - P(GA523/07/1171), P(GA523/07/1282), Z(AV0Z50520514)
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http://linked.open...iv/cisloPeriodika
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http://linked.open...vai/riv/dodaniDat
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http://linked.open...aciTvurceVysledku
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http://linked.open.../riv/druhVysledku
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http://linked.open...iv/duvernostUdaju
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http://linked.open...titaPredkladatele
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http://linked.open...dnocenehoVysledku
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http://linked.open...ai/riv/idVysledku
| - RIV/68378050:_____/08:00306812
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http://linked.open...riv/jazykVysledku
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http://linked.open.../riv/klicovaSlova
| - retrovirus receptors; avian sarcoma and leukosis virus; resistance to retrovirus (en)
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http://linked.open.../riv/klicoveSlovo
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http://linked.open...odStatuVydavatele
| - US - Spojené státy americké
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http://linked.open...ontrolniKodProRIV
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http://linked.open...i/riv/nazevZdroje
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http://linked.open...in/vavai/riv/obor
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http://linked.open...ichTvurcuVysledku
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http://linked.open...cetTvurcuVysledku
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http://linked.open...vavai/riv/projekt
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http://linked.open...UplatneniVysledku
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http://linked.open...v/svazekPeriodika
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http://linked.open...iv/tvurceVysledku
| - Plachý, Jiří
- Svoboda, Jan
- Hejnar, Jiří
- Geryk, Josef
- Reinišová, Markéta
- Šenigl, Filip
- Elleder, Daniel
- Federspiel, M. J.
- Yin, X.
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http://linked.open...ain/vavai/riv/wos
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http://linked.open...n/vavai/riv/zamer
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issn
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number of pages
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