| Attributes | Values |
|---|
| rdf:type
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| Description
| - Monoklonální protilátky (MAbs) proti DQH proteinu byly připraveny a použity pro určení původu DQH v reprodukčních orgánech kance, jeho lokalizace na spermiích a pro sledování vazebných schopností v prasečím oviduktu a se ZP vajíčka. mRNA transkript DQH byl nalezen v semenných vacích (SV) ne v testes, epididymu a prostatě. Translatované produkty byly pomocí MAbs imunodetekovány v extraktu a tekutině SV, na tkáňových řezech SV a na membráně na akrosomu ejakulovaných spermií. Výsledky potvrzují schopnost vazby DQH proteinu na povrch spermie při ejakulaci a účast na tvorbě reservoáru spermií v prasečím oviduktu. Mabs redukují také vazbu spermií k vajíčku a potvrzují tak roli DQH proteinu v primární vazbě spermie se ZP vajíčka. (cs)
- Monoclonal antibodies (MAbs) against DQH protein were prepared and used for determination of the DQH origin in boar reproductive organs, its localization on boar spermatozoa, and for investigation of its binding abilities in the porcine oviduct and to the ZP of the oocyte. The mRNA transcript of DQH was found in seminal vesicles, not in the testis, epididymis and prostate. Its translated products were immunodetected by MAbs in seminal vesicle extract and fluid, on seminal vesicle tissue sections and on the membrane-associated acrosome part of ejaculated spermatozoa. These results confirmed the ability of DQH protein to bind the sperm surface at ejaculation and to participate in the formation of the sperm reservoir in the porcine oviduct. Moreover, Mabs reduced binding of sperm to oocytes and proved the role of DQH protein in the sperm-zona pellucida primary binding.
- Monoclonal antibodies (MAbs) against DQH protein were prepared and used for determination of the DQH origin in boar reproductive organs, its localization on boar spermatozoa, and for investigation of its binding abilities in the porcine oviduct and to the ZP of the oocyte. The mRNA transcript of DQH was found in seminal vesicles, not in the testis, epididymis and prostate. Its translated products were immunodetected by MAbs in seminal vesicle extract and fluid, on seminal vesicle tissue sections and on the membrane-associated acrosome part of ejaculated spermatozoa. These results confirmed the ability of DQH protein to bind the sperm surface at ejaculation and to participate in the formation of the sperm reservoir in the porcine oviduct. Moreover, Mabs reduced binding of sperm to oocytes and proved the role of DQH protein in the sperm-zona pellucida primary binding. (en)
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| Title
| - Origin, localization and binding abilities of boar DQH sperm surface protein tested by specific monoclonal antibodies
- Origin, localization and binding abilities of boar DQH sperm surface protein tested by specific monoclonal antibodies (en)
- Původ, lokalizace a vazebné vlastnosti kančího DQH povrchového proteinu spermie testované specifickými monoklonálními protilátkami (cs)
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| skos:prefLabel
| - Origin, localization and binding abilities of boar DQH sperm surface protein tested by specific monoclonal antibodies
- Origin, localization and binding abilities of boar DQH sperm surface protein tested by specific monoclonal antibodies (en)
- Původ, lokalizace a vazebné vlastnosti kančího DQH povrchového proteinu spermie testované specifickými monoklonálními protilátkami (cs)
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| skos:notation
| - RIV/68378050:_____/07:00093159!RIV08-AV0-68378050
|
| http://linked.open.../vavai/riv/strany
| |
| http://linked.open...avai/riv/aktivita
| |
| http://linked.open...avai/riv/aktivity
| - P(1M06011), P(GA303/06/0895), P(GP303/04/P070), Z(AV0Z50520514), Z(AV0Z50520701)
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| http://linked.open...iv/cisloPeriodika
| |
| http://linked.open...vai/riv/dodaniDat
| |
| http://linked.open...aciTvurceVysledku
| |
| http://linked.open.../riv/druhVysledku
| |
| http://linked.open...iv/duvernostUdaju
| |
| http://linked.open...titaPredkladatele
| |
| http://linked.open...dnocenehoVysledku
| |
| http://linked.open...ai/riv/idVysledku
| - RIV/68378050:_____/07:00093159
|
| http://linked.open...riv/jazykVysledku
| |
| http://linked.open.../riv/klicovaSlova
| - Boar DQH sperm surface protein; monoclonal antibody; binding abilities in the porcine oviduct (en)
|
| http://linked.open.../riv/klicoveSlovo
| |
| http://linked.open...odStatuVydavatele
| |
| http://linked.open...ontrolniKodProRIV
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| http://linked.open...i/riv/nazevZdroje
| - Journal of Reproductive Immunology
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| http://linked.open...in/vavai/riv/obor
| |
| http://linked.open...ichTvurcuVysledku
| |
| http://linked.open...cetTvurcuVysledku
| |
| http://linked.open...vavai/riv/projekt
| |
| http://linked.open...UplatneniVysledku
| |
| http://linked.open...v/svazekPeriodika
| |
| http://linked.open...iv/tvurceVysledku
| - Elzeinová, Fatima
- Jonáková, Věra
- Pěknicová, Jana
- Tichá, M.
- Maňásková, Pavla
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| http://linked.open...n/vavai/riv/zamer
| |
| issn
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| number of pages
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