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| rdf:type
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| Description
| - Hyaluronidases (Hyas) play an important role in gamete interaction and fertility in mammals. We investigated multiple forms of the enzyme in boar reproductive tract by electrophoretic methods. Two forms were detected in boar seminal plasma (Mr 55 000 and 65 000) using hyaluronic acid substrate PAGE in the presence of SDS. These 2 forms differ, besides their affinity to heparin, also in the pH optimum of their enzymatic activity. The 55 000 form was active both at acidic (pH 3.7) and neutral pH (pH 7.4) and was bound to immobilized heparin. The 65 000 form was active only at acidic pH and did not interact with heparin. The Hya form active both at acidic and neutral pH (51 000) was detected in epididymal fluid. In the detergent extracts of boar sperm only one active form of the enzyme was found (55 000). Hya (65 000) was also found, besides in the non-heparin binding fraction of seminal plasma proteins, in boar seminal vesicle fluid. No Hya activity was detected in the prostate fluid.
- Hyaluronidases (Hyas) play an important role in gamete interaction and fertility in mammals. We investigated multiple forms of the enzyme in boar reproductive tract by electrophoretic methods. Two forms were detected in boar seminal plasma (Mr 55 000 and 65 000) using hyaluronic acid substrate PAGE in the presence of SDS. These 2 forms differ, besides their affinity to heparin, also in the pH optimum of their enzymatic activity. The 55 000 form was active both at acidic (pH 3.7) and neutral pH (pH 7.4) and was bound to immobilized heparin. The 65 000 form was active only at acidic pH and did not interact with heparin. The Hya form active both at acidic and neutral pH (51 000) was detected in epididymal fluid. In the detergent extracts of boar sperm only one active form of the enzyme was found (55 000). Hya (65 000) was also found, besides in the non-heparin binding fraction of seminal plasma proteins, in boar seminal vesicle fluid. No Hya activity was detected in the prostate fluid. (en)
- Hyaluronidasy (Hyas) se účastní interakce gamet a oplodnění u savců. Sledovali jsme četné formy enzymu v reprodukčním traktu kance elektroforetickými metodami. Detekovali jsme dvě formy Hya (Mr of 55 000 and 65 000) v kančí semenné plasmě pomocí PAGE za použití substrátu kys. hyaluronové v přítomnosti SDS. Tyto dvě formy se liší afinitou k heparinu, pH optimem enzymatické aktivity. 55 000 forma byla aktivní při pH 3.7 i pH 7.4 a váže se na imobilizovaný heparin. 65 000 forma byla aktivní pouze v kyselém pH a nereaguje s heparinem. Hya (51 000) aktivní v kys. i neutrálním pH byla detekována v epididymální tekutině. V extraktu kančích spermií detergentem byla nalezena aktivní Hya (55 000). Hya (65 000) byla nalezena v tekutině semených vaků kance a ve frakci proteinů kančí semenné plasmy nevázajících heparin. V tekutině prostaty nebyla zjištěna aktivita Hya. (cs)
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| Title
| - Preliminary characterization of multiple hyaluronidase forms in boar reproductive tract
- Předběžné charakterizace četných forem hyaluronidasy v reprodukčním traktu kance (cs)
- Preliminary characterization of multiple hyaluronidase forms in boar reproductive tract (en)
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| skos:prefLabel
| - Preliminary characterization of multiple hyaluronidase forms in boar reproductive tract
- Předběžné charakterizace četných forem hyaluronidasy v reprodukčním traktu kance (cs)
- Preliminary characterization of multiple hyaluronidase forms in boar reproductive tract (en)
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| skos:notation
| - RIV/68378050:_____/07:00093153!RIV08-AV0-68378050
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| http://linked.open.../vavai/riv/strany
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| http://linked.open...avai/riv/aktivita
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| http://linked.open...avai/riv/aktivity
| - P(1M06011), P(GA303/06/0895), Z(AV0Z50520514), Z(AV0Z50520701)
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| http://linked.open...iv/cisloPeriodika
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| http://linked.open...vai/riv/dodaniDat
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| http://linked.open...aciTvurceVysledku
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| http://linked.open.../riv/druhVysledku
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| http://linked.open...iv/duvernostUdaju
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| http://linked.open...titaPredkladatele
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| http://linked.open...dnocenehoVysledku
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| http://linked.open...ai/riv/idVysledku
| - RIV/68378050:_____/07:00093153
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| http://linked.open...riv/jazykVysledku
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| http://linked.open.../riv/klicovaSlova
| - hyaluronidase; boar sperm; seminal plasma; epididymis; seminal vesicle (en)
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| http://linked.open.../riv/klicoveSlovo
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| http://linked.open...odStatuVydavatele
| - US - Spojené státy americké
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| http://linked.open...ontrolniKodProRIV
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| http://linked.open...i/riv/nazevZdroje
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| http://linked.open...in/vavai/riv/obor
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| http://linked.open...ichTvurcuVysledku
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| http://linked.open...cetTvurcuVysledku
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| http://linked.open...vavai/riv/projekt
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| http://linked.open...UplatneniVysledku
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| http://linked.open...v/svazekPeriodika
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| http://linked.open...iv/tvurceVysledku
| - Jonáková, Věra
- Tichá, M.
- Maňásková, Pavla
- Cibulková, Eva
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| http://linked.open...n/vavai/riv/zamer
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| issn
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| number of pages
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