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| Description
| - The role of boar seminal plasma proteins attached to the sperm plasma membrane during ejaculation in saccharide-mediated events in the female reproductive tract has been studied. Heparin-binding proteins (DQH sperm surface protein and AQN and AWN spermadhesins) and their aggregated forms interacted more strongly with both the oviductal epithelium cells and fluid than non-heparin-binding proteins (PSP I and PSP II spermadhesins) and their heterodimer and interactions correlate with affinity of these proteins to yeast mannan. Indirect immunofluorescence showed interaction between heparin-binding proteins and mannosyl components of oviductal epithelium. We suggest that heparin-binding proteins on the sperm could enable sperm binding to oviductal epithelium and thus participate in the formation of the sperm oviductal reservoir.
- The role of boar seminal plasma proteins attached to the sperm plasma membrane during ejaculation in saccharide-mediated events in the female reproductive tract has been studied. Heparin-binding proteins (DQH sperm surface protein and AQN and AWN spermadhesins) and their aggregated forms interacted more strongly with both the oviductal epithelium cells and fluid than non-heparin-binding proteins (PSP I and PSP II spermadhesins) and their heterodimer and interactions correlate with affinity of these proteins to yeast mannan. Indirect immunofluorescence showed interaction between heparin-binding proteins and mannosyl components of oviductal epithelium. We suggest that heparin-binding proteins on the sperm could enable sperm binding to oviductal epithelium and thus participate in the formation of the sperm oviductal reservoir. (en)
- Byla studována úloha proteinů semenné plasmy, které se váží na plasmatickou membránu spermie během ejakulace, při reakcích se sacharidy v samičím reprodukčním traktu. Heparin-vázající (Hep+) proteiny (DQH povrchový protein spermie a AQN a AWN spermadhesiny) a jejich agregované formy (frakce II a III) reagují silněji jak s buňkami epitelu oviduktu, tak i s tekutinou oviduktu než heparin-nevázající (Hep-) proteiny (PSP I a PSP II spermadhesiny) a jejich heterodimer (frakce IV) a interakce korelují s afinitou těchto proteinů s mananem z kvasinky. Nepřímá imunofluorescence (IMF) ukazuje, že AQN 1 spermadhesin a frakce II se váží k jednotlivým částem oviduktu (apikální glykokalyx ampule, isthmus a spojení vejcovodu s dělohou). Předpokládáme, že Hep+ proteiny (speciálně AQN 1, fraction II) na spermii zprostředkovávají vazbu spermie k epitelu oviduktu a tak se účastní tvorby oviduktálního reservoiru spermií. (cs)
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| Title
| - Saccharide-mediated interactions of boar sperm surface proteins with components of the porcine oviduct
- Saccharide-mediated interactions of boar sperm surface proteins with components of the porcine oviduct (en)
- Interakce povrchových proteinů kančích spermií s komponenty prasečího vejcovodu (cs)
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| skos:prefLabel
| - Saccharide-mediated interactions of boar sperm surface proteins with components of the porcine oviduct
- Saccharide-mediated interactions of boar sperm surface proteins with components of the porcine oviduct (en)
- Interakce povrchových proteinů kančích spermií s komponenty prasečího vejcovodu (cs)
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| skos:notation
| - RIV/68378050:_____/06:00043126!RIV07-MZ0-68378050
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| http://linked.open.../vavai/riv/strany
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| http://linked.open...avai/riv/aktivita
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| http://linked.open...avai/riv/aktivity
| - P(GA303/06/0895), P(GP303/04/P070), P(NJ7463), Z(AV0Z50520514)
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| http://linked.open...iv/cisloPeriodika
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| http://linked.open...vai/riv/dodaniDat
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| http://linked.open...aciTvurceVysledku
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| http://linked.open.../riv/druhVysledku
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| http://linked.open...iv/duvernostUdaju
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| http://linked.open...titaPredkladatele
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| http://linked.open...dnocenehoVysledku
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| http://linked.open...ai/riv/idVysledku
| - RIV/68378050:_____/06:00043126
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| http://linked.open...riv/jazykVysledku
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| http://linked.open.../riv/klicovaSlova
| - boar seminal plasma proteins; saccharide-mediated events; sperm oviductal reservoir (en)
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| http://linked.open.../riv/klicoveSlovo
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| http://linked.open...odStatuVydavatele
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| http://linked.open...ontrolniKodProRIV
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| http://linked.open...i/riv/nazevZdroje
| - Journal of Reproductive Immunology
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| http://linked.open...in/vavai/riv/obor
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| http://linked.open...ichTvurcuVysledku
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| http://linked.open...cetTvurcuVysledku
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| http://linked.open...vavai/riv/projekt
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| http://linked.open...UplatneniVysledku
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| http://linked.open...v/svazekPeriodika
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| http://linked.open...iv/tvurceVysledku
| - Jonáková, Věra
- Liberda, J.
- Tichá, M.
- Maňásková, Pavla
- Prelovská, L.
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| http://linked.open...n/vavai/riv/zamer
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| issn
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| number of pages
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