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  • Do mezinárodního workshopu HLDA8 bylo zasláno přes 600 protilátek. Z nich 101 bylo vybráno do “slepého panelu”, který obsahoval také 5 negativních kontrol a 27 pozitivních kontrol známých CD specificit. Pro 31 vzorků byly identifikovány jejich specifity (známé CD a MHC proteiny). Bylo identifikováno několik párů velmi podobných vzorků, které pak byly srovnávány co do identity cílových antigenů pomocí Western blottingu a imunohistochemie. (cs)
  • There were over 600 antibodies submitted to HLDA8, with many of unknown specificity. Of these, 101 antibodies were selected for a blind panel study that also included 5 negative controls and 27 positive controls of known CD specificity making a total of 133 antibodies in the final panel. Of the 101 unknowns, 31 antibodies were identified during the course of this blind panel study as being specific for known molecules and included some specific for MHC class II antigens, CD45 isoforms and the Dombrock antigen. Several antibody pairs among those in the blind panel were found to have very similar staining patterns and were therefore compared by immunohistochemical and/or Western blot analyses for identity.
  • There were over 600 antibodies submitted to HLDA8, with many of unknown specificity. Of these, 101 antibodies were selected for a blind panel study that also included 5 negative controls and 27 positive controls of known CD specificity making a total of 133 antibodies in the final panel. Of the 101 unknowns, 31 antibodies were identified during the course of this blind panel study as being specific for known molecules and included some specific for MHC class II antigens, CD45 isoforms and the Dombrock antigen. Several antibody pairs among those in the blind panel were found to have very similar staining patterns and were therefore compared by immunohistochemical and/or Western blot analyses for identity. (en)
Title
  • The HLDA8 blind panel: findings and conclusions
  • Slepý panel HLDA8: poznatky a závěry (cs)
  • The HLDA8 blind panel: findings and conclusions (en)
skos:prefLabel
  • The HLDA8 blind panel: findings and conclusions
  • Slepý panel HLDA8: poznatky a závěry (cs)
  • The HLDA8 blind panel: findings and conclusions (en)
skos:notation
  • RIV/68378050:_____/05:00027620!RIV06-AV0-68378050
http://linked.open.../vavai/riv/strany
  • 75;83
http://linked.open...avai/riv/aktivita
http://linked.open...avai/riv/aktivity
  • Z(AV0Z5052915)
http://linked.open...iv/cisloPeriodika
  • 1
http://linked.open...vai/riv/dodaniDat
http://linked.open...aciTvurceVysledku
http://linked.open.../riv/druhVysledku
http://linked.open...iv/duvernostUdaju
http://linked.open...titaPredkladatele
http://linked.open...dnocenehoVysledku
  • 523441
http://linked.open...ai/riv/idVysledku
  • RIV/68378050:_____/05:00027620
http://linked.open...riv/jazykVysledku
http://linked.open.../riv/klicovaSlova
  • CD molecules; leukocyte antigen (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...odStatuVydavatele
  • NL - Nizozemsko
http://linked.open...ontrolniKodProRIV
  • [28E03DCCBB91]
http://linked.open...i/riv/nazevZdroje
  • Journal of Immunological Methods
http://linked.open...in/vavai/riv/obor
http://linked.open...ichTvurcuVysledku
http://linked.open...cetTvurcuVysledku
http://linked.open...UplatneniVysledku
http://linked.open...v/svazekPeriodika
  • 305
http://linked.open...iv/tvurceVysledku
  • Angelisová, Pavla
  • Hořejší, Václav
  • Drbal, Karel
  • Bailey, S.
  • Zola, H.
  • Macardle, P.
  • Swart, B.
  • Dagdeviren, A.
  • Dandie, G. W.
  • Hodge, G.
  • Hollemweguer, E.
  • Milford, E. L.
  • Millard, D.
  • Nairn, J.
  • Salganik, M. P.
  • Tinckam, K.
  • Wand, M. P.
http://linked.open...n/vavai/riv/zamer
issn
  • 0022-1759
number of pages
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