| Attributes | Values |
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| rdf:type
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| Description
| - c-Myb, known to play a central role in hematopoiesis, is also an important factor involved in myogenesis. Here, we found that both c-myb and its alternatively spliced form c-mybE9A are expressed in proliferating C2C12 myoblasts and turned off in differentiating cells. Constitutive expression of exogenous c-myb in C2C12 cells inhibits their terminal differentiation. It is shown that the c-Myb protein physically interacts with MyoD, the key regulator of myogenesis, and inhibits MyoD-dependent transcription. The interaction domains are the DNA binding domain of c-Myb and the bHLH motif of MyoD. Our data suggest that in proliferating cells c-Myb binds MyoD and inhibits its transcriptional activity until cell-cell contacts are established and c-myb expression is switched off. Thus, the c-Myb protein may be one of factors ensuring that proliferating myoblasts remain undifferentiated.
- c-Myb, known to play a central role in hematopoiesis, is also an important factor involved in myogenesis. Here, we found that both c-myb and its alternatively spliced form c-mybE9A are expressed in proliferating C2C12 myoblasts and turned off in differentiating cells. Constitutive expression of exogenous c-myb in C2C12 cells inhibits their terminal differentiation. It is shown that the c-Myb protein physically interacts with MyoD, the key regulator of myogenesis, and inhibits MyoD-dependent transcription. The interaction domains are the DNA binding domain of c-Myb and the bHLH motif of MyoD. Our data suggest that in proliferating cells c-Myb binds MyoD and inhibits its transcriptional activity until cell-cell contacts are established and c-myb expression is switched off. Thus, the c-Myb protein may be one of factors ensuring that proliferating myoblasts remain undifferentiated. (en)
- c-Myb, o němž je známo že hraje centrální roli v krvetvorbě, je také důležitý faktor v myogenezi. Zjistili jsme, že jak c-myb, tak jeho alternativně sestřižená forma c-mybE9A je exprimována v dělících se C2C12 myoblastech, v diferencujících buňkách je jejich exprese vypnuta. Vynucená exprese vloženého c-myb genu zablokuje diferenciaci C2C12 buněk. Je ukázáno, že c-Myb protein se fyzicky váže na MyoD, hlavní faktor regulující myogenezi a inhibuje transkripci, která je závislá na MyoD. Vazebné oblasti jsou DNA vazebná doména c-Mybu a bHLH oblast MyoD. Na základě našich výsledků předpokládáme, že c-Myb se váže na MyoD a inhibuje jeho aktivitu až do doby než se buňky dostanou do vzájemného kontaktu a exprese c-mybu se vypne. c-Myb tak může být jedním z faktorů zajištující že dělící se myoblasty nemohou diferencovat. (cs)
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| Title
| - c-Myb inhibits myogenic differentiation through repression of MyoD
- c-Myb inhibuje svalovou diferenciaci pomocí represe MyoD (cs)
- c-Myb inhibits myogenic differentiation through repression of MyoD (en)
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| skos:prefLabel
| - c-Myb inhibits myogenic differentiation through repression of MyoD
- c-Myb inhibuje svalovou diferenciaci pomocí represe MyoD (cs)
- c-Myb inhibits myogenic differentiation through repression of MyoD (en)
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| skos:notation
| - RIV/68378050:_____/05:00027483!RIV06-AV0-68378050
|
| http://linked.open.../vavai/riv/strany
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| http://linked.open...avai/riv/aktivita
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| http://linked.open...avai/riv/aktivity
| - P(GA304/03/0463), Z(AV0Z50520514)
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| http://linked.open...iv/cisloPeriodika
| |
| http://linked.open...vai/riv/dodaniDat
| |
| http://linked.open...aciTvurceVysledku
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| http://linked.open.../riv/druhVysledku
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| http://linked.open...iv/duvernostUdaju
| |
| http://linked.open...titaPredkladatele
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| http://linked.open...dnocenehoVysledku
| |
| http://linked.open...ai/riv/idVysledku
| - RIV/68378050:_____/05:00027483
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| http://linked.open...riv/jazykVysledku
| |
| http://linked.open.../riv/klicovaSlova
| - c-Myb; MyoD; myogenesis (en)
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| http://linked.open.../riv/klicoveSlovo
| |
| http://linked.open...odStatuVydavatele
| - US - Spojené státy americké
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| http://linked.open...ontrolniKodProRIV
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| http://linked.open...i/riv/nazevZdroje
| - Experimental Cell Research
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| http://linked.open...in/vavai/riv/obor
| |
| http://linked.open...ichTvurcuVysledku
| |
| http://linked.open...cetTvurcuVysledku
| |
| http://linked.open...vavai/riv/projekt
| |
| http://linked.open...UplatneniVysledku
| |
| http://linked.open...v/svazekPeriodika
| |
| http://linked.open...iv/tvurceVysledku
| - Dvořák, Michal
- Kašpar, Petr
- Sedlák, David
- Pajer, Petr
- Tamaoki, T.
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| http://linked.open...n/vavai/riv/zamer
| |
| issn
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| number of pages
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