About: Polyomavirus EGFP-pseudocapsids:analysis of model particles for introduction of proteins and pepetides into mammalian cells

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	A vector for preparation of mouse polyomavirus capsid-like particles for transfer of foreign peptides or proteins into cells was constructed. Model pseudocapsids carrying EGFP fused with the C-terimal part of the VP3 minor protein (EGFP-VLPs) have been prepared and analysed for their ability to be internalised and processed by mouse cells and to activate mouse and human dendritic cells (DC) in vitro. EGFP-VLPs entered mouse epithelial cells, fibroblasts and human and mouse DC efficiently and were processed by both, lysosomes and proteasomes. Surprisingly, they did not induce upregulation of DC co-stimulation molecules or maturation markers in vitro; however, they did induce interleukin 12 secretion. A vector for preparation of mouse polyomavirus capsid-like particles for transfer of foreign peptides or proteins into cells was constructed. Model pseudocapsids carrying EGFP fused with the C-terimal part of the VP3 minor protein (EGFP-VLPs) have been prepared and analysed for their ability to be internalised and processed by mouse cells and to activate mouse and human dendritic cells (DC) in vitro. EGFP-VLPs entered mouse epithelial cells, fibroblasts and human and mouse DC efficiently and were processed by both, lysosomes and proteasomes. Surprisingly, they did not induce upregulation of DC co-stimulation molecules or maturation markers in vitro; however, they did induce interleukin 12 secretion. (en) Byl zkonstruován vektor myších polyomavirových kapsid-like částic pro přenos cizorodých peptidů a proteinů do buněk. Byly připraveny modelové pseudokapsidy nesoucí EGFP fuzovaný s C-terminální částí minoritního proteinu VP3 (EGFP-VPLs) a byla analyzována jejich schopnost internalizace a zpracování myšími buňkami jakož i schopnost aktivace myších a lidských dendritických buněk in vitro. EGFP-VPLs účinně pronikly do myších epiteliálních buněk, fibroblastů a lidských i myších dendritických buněk a byly upravovány jak lysozomy tak proteazomy. Překvapivě však neindukovaly stimulaci molekul a maturačních markerů in vitro, ale indukovaly sekreci interleukinu 12. (cs)
Title	Polyomavirus EGFP-pseudocapsids:analysis of model particles for introduction of proteins and pepetides into mammalian cells Polyomavirus EGFP-pseudocapsids:analysis of model particles for introduction of proteins and pepetides into mammalian cells (en) Pseudokapsidy polyoma viru EGFP: analýza modelových partikulí pro vnášení proteinů a peptidů do savčích buněk (cs)
skos:prefLabel	Polyomavirus EGFP-pseudocapsids:analysis of model particles for introduction of proteins and pepetides into mammalian cells Polyomavirus EGFP-pseudocapsids:analysis of model particles for introduction of proteins and pepetides into mammalian cells (en) Pseudokapsidy polyoma viru EGFP: analýza modelových partikulí pro vnášení proteinů a peptidů do savčích buněk (cs)
skos:notation	RIV/68378050:_____/05:00026205!RIV06-AV0-68378050
http://linked.open.../vavai/riv/strany	6549;6558
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(GA204/03/0593), P(NC6957), Z(AV0Z50520514)
http://linked.open...iv/cisloPeriodika	29
http://linked.open...vai/riv/dodaniDat	2006
http://linked.open...aciTvurceVysledku	Frič, Jan Holáň, Vladimír Štokrová, Jitka
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Ústav molekulární genetiky AV ČR, v. v. i.
http://linked.open...dnocenehoVysledku	536702
http://linked.open...ai/riv/idVysledku	RIV/68378050:_____/05:00026205
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	mouse polyomavirus; empty artificial virus-like particle; dendritic cell activation (en)
http://linked.open.../riv/klicoveSlovo	mouse polyomavirus dendritic cell activation empty artificial virus-like particle
http://linked.open...odStatuVydavatele	GB - Spojené království Velké Británie a Severního Irska
http://linked.open...ontrolniKodProRIV	[E17BEC8AE34D]
http://linked.open...i/riv/nazevZdroje	FEBS Letters
http://linked.open...in/vavai/riv/obor	EB
http://linked.open...ichTvurcuVysledku	3 (xsd:int)
http://linked.open...cetTvurcuVysledku	8 (xsd:int)
http://linked.open...vavai/riv/projekt	Immunotherapy of chronic myeloid leukemia: therapeutic vaccines in a mouse model and preparation of a clinical study Studies of molecular mechanism of polyomavirus infection aimed to the development of capsid-like particles for the gene delivery systém
http://linked.open...UplatneniVysledku	2005
http://linked.open...v/svazekPeriodika	579
http://linked.open...iv/tvurceVysledku	Forstová, J. Holáň, Vladimír Štokrová, Jitka Marek, M. Spíšek, R. Frič, Jan Bouřa, E. Liebl, D.
http://linked.open...n/vavai/riv/zamer	Molekulárně genetické a buněčné základy klíčových biologických procesů: genová exprese, onkogeneze, replikace virů, imunita a vývoj organismů
issn	0014-5793
number of pages	10 (xsd:int)

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