Description
| - The majority of pancreatic and colorectal tumors expressed the a2, a3, a6, b1, and b4 integrins and the tetraspanins CD9, CD63, CD81, CD151, and CO-029. Expression of a6b4 and CO-029 was restricted to tumor cells, whereas a1, a2, a3, a6, b1, and CD9, CD81, CD151 were also expressed by the surrounding stroma. CD63, CD81, and b1 expression was observed at comparably high levels in healthy pancreatic tissue. al3b1 frequently colocalized and coimmunoprecipitated with CD9, CD81, and CD151, whereas a6b4 colocalized and coimmunoprecipitated mostly with CD151 and CO-029. Notably, protein kinase C activation strengthened only the colocalization of CD151 and CO-029 with b4 and was accompanied by internalization of the integrin-tetraspanin complex, decreased laminin 5 adhesion, and increased cell migration. CONCLUSION: Integrin a6b4 is selectively up-regulated in pancreatic and colorectal cancer. The association of a6b4 with CD151 and CO-029 correlates with increased tumor cell motility.
- The majority of pancreatic and colorectal tumors expressed the a2, a3, a6, b1, and b4 integrins and the tetraspanins CD9, CD63, CD81, CD151, and CO-029. Expression of a6b4 and CO-029 was restricted to tumor cells, whereas a1, a2, a3, a6, b1, and CD9, CD81, CD151 were also expressed by the surrounding stroma. CD63, CD81, and b1 expression was observed at comparably high levels in healthy pancreatic tissue. al3b1 frequently colocalized and coimmunoprecipitated with CD9, CD81, and CD151, whereas a6b4 colocalized and coimmunoprecipitated mostly with CD151 and CO-029. Notably, protein kinase C activation strengthened only the colocalization of CD151 and CO-029 with b4 and was accompanied by internalization of the integrin-tetraspanin complex, decreased laminin 5 adhesion, and increased cell migration. CONCLUSION: Integrin a6b4 is selectively up-regulated in pancreatic and colorectal cancer. The association of a6b4 with CD151 and CO-029 correlates with increased tumor cell motility. (en)
- Pankreatické a kolorektální tumory exprimují integriny a2, a3, a6, b1, b4 a tetraspaniny CD9, CD63, CD81, CD151, CO-029. Exprese a6b4 a CO-029 byla omezena na nádorové buňky, zatímco a1, a2, a3, a6, b1, and CD9, CD81, CD151 byly přítomny také na stromatu. CD63, CD81, a b1 byly přítomny na zdravé pankreatické tkáni. al3b1 kolokalizoval s CD9, CD81, a CD151, zatímco a6b4 s CD151 a CO-029. Aktivace PKC zesilovala kolokalizaci CD151 a CO-029 s b4 a byla doprovázena internalizací komplexu integrin-tetraspanin, sníženou adhezí k lamininu 5, a zvýšenou motilitou buněk. Závěr - Integrin a6b4 je selektivně up-regulován v pankreatických a kolorektálních tumorech. Asociace a6b4 s CD151 a CO-029 koreluje se zvýšenou motilitou nádorových buněk. (cs)
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