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Description
| - The monoclonal antibody 1696, elicited by HIV-1 protease, inhibits the activity of both HIV-1 and HIV-2 proteases with inhibition constants in the low nanomolar range. The antibody cross-reacts with peptides derived from the N-terminal region of both proteases. The crystal structure of the recombinant single-chain Fv fragment of 1696 complexed with an N-terminal peptide from the HIV-2 protease has been determined at 1.88A resolution. Interactions of the peptide with scFv1696 are compared with the previously reported structure of scFv1696 in complex with the corresponding peptide from HIV-1 protease. The origin of cross-reactivity of mAb1696 with HIV proteases is discussed.
- The monoclonal antibody 1696, elicited by HIV-1 protease, inhibits the activity of both HIV-1 and HIV-2 proteases with inhibition constants in the low nanomolar range. The antibody cross-reacts with peptides derived from the N-terminal region of both proteases. The crystal structure of the recombinant single-chain Fv fragment of 1696 complexed with an N-terminal peptide from the HIV-2 protease has been determined at 1.88A resolution. Interactions of the peptide with scFv1696 are compared with the previously reported structure of scFv1696 in complex with the corresponding peptide from HIV-1 protease. The origin of cross-reactivity of mAb1696 with HIV proteases is discussed. (en)
- Monoklonální protilátka 1696, jejíž tvorbu vyvolává proteáza HIV-1, potlačuje aktivitu proteáz HIV-1 i HIV-2, s inhibičními konstantami v nízkém nanomolárním rozmezí. Tato protilátka křížově reaguje s peptidy odvozenými od N-koncové oblasti obou proteáz. Byla určena krystalová struktura rekombinantního jednořetězcového Fv fragmentu 1696 v komplexu s N-koncovým peptidem proteázy HIV-2 s rozlišením 1,88A. Byly porovnány vzájemné reakce tohoto peptidu s scFv1696 a dříve určené struktury scFv1696 v komplexu s odpovídajícím peptidem proteázy HIV-1. Je diskutován původ křížové reakce monoklonální protilátky 1696 s proteázami HIV. (cs)
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Title
| - Crystal structure of a cross-reaction complex between an anti-HIV-1 protease antibody and an HIV-2 protease peptide
- Crystal structure of a cross-reaction complex between an anti-HIV-1 protease antibody and an HIV-2 protease peptide (en)
- Krystalová struktura křížově reagujícího komplexu protilátky proti HIV-1 proteáze a peptidu proteázy HIV-2 (cs)
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skos:prefLabel
| - Crystal structure of a cross-reaction complex between an anti-HIV-1 protease antibody and an HIV-2 protease peptide
- Crystal structure of a cross-reaction complex between an anti-HIV-1 protease antibody and an HIV-2 protease peptide (en)
- Krystalová struktura křížově reagujícího komplexu protilátky proti HIV-1 proteáze a peptidu proteázy HIV-2 (cs)
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skos:notation
| - RIV/68378050:_____/05:00001369!RIV06-AV0-68378050
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http://linked.open.../vavai/riv/strany
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http://linked.open...avai/riv/aktivita
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http://linked.open...avai/riv/aktivity
| - P(GV203/98/K023), P(KSK5011112)
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http://linked.open...iv/cisloPeriodika
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http://linked.open...vai/riv/dodaniDat
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http://linked.open...aciTvurceVysledku
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http://linked.open.../riv/druhVysledku
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http://linked.open...iv/duvernostUdaju
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http://linked.open...titaPredkladatele
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http://linked.open...dnocenehoVysledku
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http://linked.open...ai/riv/idVysledku
| - RIV/68378050:_____/05:00001369
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http://linked.open...riv/jazykVysledku
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http://linked.open.../riv/klicovaSlova
| - single-chain Fv fragment; peptide complex; HIV protease (en)
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http://linked.open.../riv/klicoveSlovo
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http://linked.open...odStatuVydavatele
| - US - Spojené státy americké
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http://linked.open...ontrolniKodProRIV
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http://linked.open...i/riv/nazevZdroje
| - Journal of Structural Biology
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http://linked.open...in/vavai/riv/obor
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http://linked.open...ichTvurcuVysledku
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http://linked.open...cetTvurcuVysledku
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http://linked.open...vavai/riv/projekt
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http://linked.open...UplatneniVysledku
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http://linked.open...v/svazekPeriodika
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http://linked.open...iv/tvurceVysledku
| - Brynda, Jiří
- Fábry, Milan
- Sieglová, Irena
- Řezáčová, Pavlína
- Sedláček, Juraj
- Hořejší, Magdalena
- Bentley, G. A.
- Lescar, J.
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issn
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number of pages
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