About: Identification and isolation of boar sperm specific antigens with potential role in sperm-egg interaction

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About: Identification and isolation of boar sperm specific antigens with potential role in sperm-egg interaction Goto Sponge NotDistinct Permalink

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Studium specifických a funkčně aktivních antigenů spermií by mohlo napomoci objasnit mechanismy interakce gamet a najít nové postupu prognózy a regulace plodnosti. Využili jsme polyklonální králičí protikančí protilátku (RABSA) k dvojité imunodifúzi, elektroforéze, imunofluorescenčnímu testu a testu ELISA. Protilátka RABSA barvila akrozomy. RABSA je specificky lokalizován ve spermatických strukturách. U Westernova přenosu rozpoznávala RABSA specificky antigen s molekulovou hmotností 60 kDa (Ag60). Biologické a IIF experimenty prokazují, že Ag60 se účastní funkčních kroků nezbytných pro vazbu spermie na vajíčko a/nebo jejich fúzi, ale ne pro vazbu spermie na zonu pellucidu (cs) Investigations on specific and functionally active sperm antigens would bring about the elucidation of the mechanisms of gamete interaction and help the search to new approaches for prognosis and regulation of fertility.We used polyclonal rabbit anti-boar spermatozoa antibody (RABSA)by double immunodiffusion, electrophoresis, immunofluorescence test and ELISA. The RABSA stained acrosomes. The RABSA is specifically localized in sperm structures. In WB RABSA recognized specifically antigen with molecular mass 60 kDa (Ag60). The biological and IIF experiments provide evidence supporting the involvement of Ag60 in functional steps required for sperm-egg binding and/or fusion, but not sperm-zona pellucida binding Investigations on specific and functionally active sperm antigens would bring about the elucidation of the mechanisms of gamete interaction and help the search to new approaches for prognosis and regulation of fertility.We used polyclonal rabbit anti-boar spermatozoa antibody (RABSA)by double immunodiffusion, electrophoresis, immunofluorescence test and ELISA. The RABSA stained acrosomes. The RABSA is specifically localized in sperm structures. In WB RABSA recognized specifically antigen with molecular mass 60 kDa (Ag60). The biological and IIF experiments provide evidence supporting the involvement of Ag60 in functional steps required for sperm-egg binding and/or fusion, but not sperm-zona pellucida binding (en)
Title	Identification and isolation of boar sperm specific antigens with potential role in sperm-egg interaction Identifikace a izolace kančích antigenů specifických pro spermie s potenciální úlohou v interakci spermie s vajíčkem (cs) Identification and isolation of boar sperm specific antigens with potential role in sperm-egg interaction (en)
skos:prefLabel	Identification and isolation of boar sperm specific antigens with potential role in sperm-egg interaction Identifikace a izolace kančích antigenů specifických pro spermie s potenciální úlohou v interakci spermie s vajíčkem (cs) Identification and isolation of boar sperm specific antigens with potential role in sperm-egg interaction (en)
skos:notation	RIV/68378050:_____/04:00105370!RIV/2005/GA0/A23005/N
http://linked.open.../vavai/riv/strany	91;106
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(GA204/02/1373), P(NJ7463), Z(AV0Z5052915)
http://linked.open...iv/cisloPeriodika	1-2
http://linked.open...vai/riv/dodaniDat	2005
http://linked.open...aciTvurceVysledku	Pěknicová, Jana
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Ústav molekulární genetiky AV ČR, v. v. i.
http://linked.open...dnocenehoVysledku	567134
http://linked.open...ai/riv/idVysledku	RIV/68378050:_____/04:00105370
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	sperm-egg interaction; antibodies (en)
http://linked.open.../riv/klicoveSlovo	sperm-egg interaction antibodies
http://linked.open...odStatuVydavatele	GB - Spojené království Velké Británie a Severního Irska
http://linked.open...ontrolniKodProRIV	[0BD1DDFD6626]
http://linked.open...i/riv/nazevZdroje	Journal of Reproductive Immunology
http://linked.open...in/vavai/riv/obor	EB
http://linked.open...ichTvurcuVysledku	1 (xsd:int)
http://linked.open...cetTvurcuVysledku	5 (xsd:int)
http://linked.open...vavai/riv/projekt	Male infertility, sperm and seminal plasma status, damage biomarkers. Sperm proteins and ubiquitination of acromose in human sperm with normal and pathological spermiogram
http://linked.open...UplatneniVysledku	2004
http://linked.open...v/svazekPeriodika	64
http://linked.open...iv/tvurceVysledku	Pěknicová, Jana Ivanova, M. Mollova, M. Stamenova, M. Baracova, V.
http://linked.open...n/vavai/riv/zamer	Regulace a signální cesty uplatňující se v genové expresi, imunitě, onkogenezi, replikaci virů, tvorbě buněčných struktur, chování buněk, vývoji a oplození
issn	0165-0378
number of pages	16 (xsd:int)

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