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| rdf:type
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| Description
| - Soubor šesti protilátek rozpoznávajících epitopy v různých oblastech molekuly gama-tubulinu byl využit k charakterizaci a lokalizaci gama-tubulinu během buněčného cyklu u Leishmania promastigotes. Imunofluorescenční mikroskopie odhalila přítomnost gama-tubulinu v bazálních tělíscích, posteriorním pólu buňky a v bičíku. Kromě toho ukázaly protilátky bodové zbarvení v subpelikulárním mikrotubulu. Toto složité schéma lokalizace bylo pozorováno u buněk v interfázi i u dělících se buněk, kde zbarvení posteriorních pólů a subpelikulárního korzetu bylo výraznější. V posteriorních pólech byla distribuce gama-tubulinu společná s proteinem interagujícím s mikrotubuly o 57 kDa, který byl imunodetekován protilátkou proti vimentinu. Elektronová mikroskopie imunodetekce zlatem na tenkých řezech izolovaných bičíků ukázala, že gama-tubulin je spojen s paraflagelární lištou (PFR), která probíhá souběžně s axonemálními mikrotubuly. Za různých extrakčních podmínek byl gama-tubulin u Leishmania nalezen... (cs)
- A panel of six anti-peptide antibodies recognizing epitopes in different regions of the -tubulin molecule was used for the characterization and localization of -tubulin during cell cycle in Leishmania promastigotes. Immunofluorescence microscopy revealed the presence of -tubulin in the basal bodies, posterior pole of the cell, and in the flagellum. Furthermore, the antibodies showed punctuate staining in the subpellicular microtubule. This complex localization pattern was observed in both interphase and dividing cells, where staining of posterior poles and the subpellicular corset was more prominent. In posterior poles, -tubulin co-distributed with the 210-kDa microtubule-interacting protein and the 57-kDa protein immunodetected with anti-vimentin antibody. Immunogold electron microscopy on thin sections of isolated flagella showed that -tubulin was associated with the paraflagellar rod (PFR) that runs adjacent to the axonemal microtubules. Under different extraction conditions, -tu...
- A panel of six anti-peptide antibodies recognizing epitopes in different regions of the -tubulin molecule was used for the characterization and localization of -tubulin during cell cycle in Leishmania promastigotes. Immunofluorescence microscopy revealed the presence of -tubulin in the basal bodies, posterior pole of the cell, and in the flagellum. Furthermore, the antibodies showed punctuate staining in the subpellicular microtubule. This complex localization pattern was observed in both interphase and dividing cells, where staining of posterior poles and the subpellicular corset was more prominent. In posterior poles, -tubulin co-distributed with the 210-kDa microtubule-interacting protein and the 57-kDa protein immunodetected with anti-vimentin antibody. Immunogold electron microscopy on thin sections of isolated flagella showed that -tubulin was associated with the paraflagellar rod (PFR) that runs adjacent to the axonemal microtubules. Under different extraction conditions, -tu... (en)
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| Title
| - Gamma-tubulin in Leishmania: cell cycle-dependent changes in subcellular localization and heterogeneity of its isoforms
- Gama-tubulin u Leishmania: změny jeho lokalizace v buňce a heterogenity jeho izoforem závislé na buněčném cyklu (cs)
- Gamma-tubulin in Leishmania: cell cycle-dependent changes in subcellular localization and heterogeneity of its isoforms (en)
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| skos:prefLabel
| - Gamma-tubulin in Leishmania: cell cycle-dependent changes in subcellular localization and heterogeneity of its isoforms
- Gama-tubulin u Leishmania: změny jeho lokalizace v buňce a heterogenity jeho izoforem závislé na buněčném cyklu (cs)
- Gamma-tubulin in Leishmania: cell cycle-dependent changes in subcellular localization and heterogeneity of its isoforms (en)
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| skos:notation
| - RIV/68378050:_____/04:00105290!RIV/2005/MSM/A23005/N
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| http://linked.open.../vavai/riv/strany
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| http://linked.open...avai/riv/aktivita
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| http://linked.open...avai/riv/aktivity
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| http://linked.open...iv/cisloPeriodika
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| http://linked.open...vai/riv/dodaniDat
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| http://linked.open...aciTvurceVysledku
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| http://linked.open.../riv/druhVysledku
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| http://linked.open...iv/duvernostUdaju
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| http://linked.open...titaPredkladatele
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| http://linked.open...dnocenehoVysledku
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| http://linked.open...ai/riv/idVysledku
| - RIV/68378050:_____/04:00105290
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| http://linked.open...riv/jazykVysledku
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| http://linked.open.../riv/klicovaSlova
| - gamma-tubulin;cell cycle;Leishmania (en)
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| http://linked.open.../riv/klicoveSlovo
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| http://linked.open...odStatuVydavatele
| - US - Spojené státy americké
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| http://linked.open...ontrolniKodProRIV
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| http://linked.open...i/riv/nazevZdroje
| - Experimental cell research
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| http://linked.open...in/vavai/riv/obor
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| http://linked.open...ichTvurcuVysledku
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| http://linked.open...cetTvurcuVysledku
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| http://linked.open...vavai/riv/projekt
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| http://linked.open...UplatneniVysledku
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| http://linked.open...v/svazekPeriodika
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| http://linked.open...iv/tvurceVysledku
| - Hozák, Pavel
- Dráber, Pavel
- Sulimenko, Tetyana
- Sulimenko, Vadym
- Libusová, Lenka
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| issn
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| number of pages
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