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  • Most of proteins of boar seminal plasma are present in this medium under physiological conditions in the form of aggregates and not of monomers. In the present communication we have shown that not only spermadhesins, but also proteinase inhibitors participate in the formation of associated complexes. Proteinase inhibitors were found after gel chromatographic separation mainly in the fraction with the lowest relative molecular mass. Two different types of serine proteinase inhibitors were isolated from boar seminal plasma ů (i) glycosylated inhibitor with Mr 12 kDa, (ii) non-glycosylated sperm-associated acrosin inhibitor with Mr 8 kDa, and a new type of low molecular weight protein that is probably composed of two subunits coupled by the -S-S- bridge. The proteinase inhibitor with Mr 8 kDa was proved to form a heterodimer with AQN 1 spermadhesin under physiological conditions, whereas inhibitor with Mr 12 kDa forms homodimers.
  • Most of proteins of boar seminal plasma are present in this medium under physiological conditions in the form of aggregates and not of monomers. In the present communication we have shown that not only spermadhesins, but also proteinase inhibitors participate in the formation of associated complexes. Proteinase inhibitors were found after gel chromatographic separation mainly in the fraction with the lowest relative molecular mass. Two different types of serine proteinase inhibitors were isolated from boar seminal plasma ů (i) glycosylated inhibitor with Mr 12 kDa, (ii) non-glycosylated sperm-associated acrosin inhibitor with Mr 8 kDa, and a new type of low molecular weight protein that is probably composed of two subunits coupled by the -S-S- bridge. The proteinase inhibitor with Mr 8 kDa was proved to form a heterodimer with AQN 1 spermadhesin under physiological conditions, whereas inhibitor with Mr 12 kDa forms homodimers. (en)
Title
  • Characterization of peptide proteinase inhibitors isolated from boar seminal plasma.
  • Characterization of peptide proteinase inhibitors isolated from boar seminal plasma. (en)
skos:prefLabel
  • Characterization of peptide proteinase inhibitors isolated from boar seminal plasma.
  • Characterization of peptide proteinase inhibitors isolated from boar seminal plasma. (en)
skos:notation
  • RIV/68378050:_____/03:23033106!RIV/2004/GA0/A23004/N
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  • P(GA303/02/0433), P(GP303/02/P069), P(NJ7463), Z(AV0Z4055905), Z(AV0Z5052915)
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  • 601522
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  • RIV/68378050:_____/03:23033106
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  • proteinase inhibitors, aggregated forms, boar seminal plasma (en)
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  • [92D3EF308478]
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  • Praha
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  • (1)
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  • Characterization of peptide proteinase inhibitors isolated from boar seminal plasma.
http://linked.open...in/vavai/riv/obor
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  • Jonáková, Věra
  • Tichá, M.
  • Jelínková, Petra
http://linked.open...n/vavai/riv/zamer
number of pages
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  • Visit, s.r.o.
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  • 80-86241-20-3
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