About: Valosine vontaining protein is a substrate of c-AMP-activated boar sperm tyrosine kinase.

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About: Valosine vontaining protein is a substrate of c-AMP-activated boar sperm tyrosine kinase. Goto Sponge NotDistinct Permalink

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Previously we reported that treatment of boar sperm with cAMP-elevating drugs induces tyrosine phosphorylation of a triton-insoluble 93 kDa protein (p93). We have isolated p93 by preparative SDS electrophoresis and blotting from urea-extracted boar sperm and identified it as a valosine containing protein (VCP) by mass spectrometry and microsequencing. With the use of antibodies to VCP and phosphotyrosine (pY) we found that both p93 and VCP are poorly extractable with triton and are solubilized in > 6 M urea. Furthermore, VCP and p93 overlap on one and two dimensional (1 and 2D) electrophoretic gels, supporting the identity of p93 as a tyrosine-phosphorylated population of VCP. According to immunofluorescence, VCP is localized along the entire sperm tail, in the posterior ring, distal equatorial segment, and postacrosome. In addition, 9-12% sperm contained VCP in the acrosome. The cAMP-elevating treatment did not alter VCP localization but induced tail tyrosine phosphorylation in 15% sperm cells. In th Previously we reported that treatment of boar sperm with cAMP-elevating drugs induces tyrosine phosphorylation of a triton-insoluble 93 kDa protein (p93). We have isolated p93 by preparative SDS electrophoresis and blotting from urea-extracted boar sperm and identified it as a valosine containing protein (VCP) by mass spectrometry and microsequencing. With the use of antibodies to VCP and phosphotyrosine (pY) we found that both p93 and VCP are poorly extractable with triton and are solubilized in > 6 M urea. Furthermore, VCP and p93 overlap on one and two dimensional (1 and 2D) electrophoretic gels, supporting the identity of p93 as a tyrosine-phosphorylated population of VCP. According to immunofluorescence, VCP is localized along the entire sperm tail, in the posterior ring, distal equatorial segment, and postacrosome. In addition, 9-12% sperm contained VCP in the acrosome. The cAMP-elevating treatment did not alter VCP localization but induced tail tyrosine phosphorylation in 15% sperm cells. In th (en)
Title	Valosine vontaining protein is a substrate of c-AMP-activated boar sperm tyrosine kinase. Valosine vontaining protein is a substrate of c-AMP-activated boar sperm tyrosine kinase. (en)
skos:prefLabel	Valosine vontaining protein is a substrate of c-AMP-activated boar sperm tyrosine kinase. Valosine vontaining protein is a substrate of c-AMP-activated boar sperm tyrosine kinase. (en)
skos:notation	RIV/68378050:_____/02:23033151!RIV/2004/GA0/A23004/N
http://linked.open.../vavai/riv/strany	366;375
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(GA204/02/1373), P(GA303/00/1651), P(NJ5851), Z(AV0Z5052915)
http://linked.open...iv/cisloPeriodika	63
http://linked.open...vai/riv/dodaniDat	2004
http://linked.open...aciTvurceVysledku	Pěknicová, Jana Geussová, Gizela
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Ústav molekulární genetiky AV ČR, v. v. i.
http://linked.open...dnocenehoVysledku	668381
http://linked.open...ai/riv/idVysledku	RIV/68378050:_____/02:23033151
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	gamete biology; spermatozoa, capacitation; phosphorylation (en)
http://linked.open.../riv/klicoveSlovo	capacitation gamete biology phosphorylation spermatozoa
http://linked.open...odStatuVydavatele	US - Spojené státy americké
http://linked.open...ontrolniKodProRIV	[BCF3BF4C58E1]
http://linked.open...i/riv/nazevZdroje	Molecular reproduction and development
http://linked.open...in/vavai/riv/obor	EB
http://linked.open...ichTvurcuVysledku	2 (xsd:int)
http://linked.open...cetTvurcuVysledku	3 (xsd:int)
http://linked.open...ocetUcastnikuAkce	0 (xsd:int)
http://linked.open...nichUcastnikuAkce	0 (xsd:int)
http://linked.open...vavai/riv/projekt	Xenoestrogens and phytoestrogens; reproductive toxicity and biomarkers Effect of endocrine disruptors on mammalian fertility Sperm proteins and ubiquitination of acromose in human sperm with normal and pathological spermiogram
http://linked.open...UplatneniVysledku	2002
http://linked.open...v/svazekPeriodika	2002
http://linked.open...iv/tvurceVysledku	Pěknicová, Jana Kalab, P. Geussová, Gizela
http://linked.open...n/vavai/riv/zamer	Regulace a signální cesty uplatňující se v genové expresi, imunitě, onkogenezi, replikaci virů, tvorbě buněčných struktur, chování buněk, vývoji a oplození
issn	1040-452X
number of pages	10 (xsd:int)

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