| Attributes | Values |
|---|
| rdf:type
| |
| Description
| - The interaction of bull seminal plasma proteins and sperm with mannan was investigated using an enzyme-linked binding assay (ELBA). A high mannan-binding activity was found in the protein fraction interacting with heparin. Mannan binding to seminal plasma proteins was inhibited by D-mannose and D-fructose, but not by D-mannose-6-phosphate, D-glucose-6-phosphate, ovalbumin and ovomucoid. Mannan inhibited the binding of bovine zona pellucida glycoproteins both to bull sperm and seminal plasma proteins. Yeast mannan immobilized to divinyl sulfone-activated Sepharose was used for the isolation of mannan-binding proteins. The protein components of this fraction were identified on the basis of relative molecular mass determination and N-terminal amino acid sequencing: RNAase dimer, PDC-109 and a protein homologous to BSP-30K (relative molecular mass 14,500). The isolated proteins were characterized by a high zona pellucida binding activity.
- The interaction of bull seminal plasma proteins and sperm with mannan was investigated using an enzyme-linked binding assay (ELBA). A high mannan-binding activity was found in the protein fraction interacting with heparin. Mannan binding to seminal plasma proteins was inhibited by D-mannose and D-fructose, but not by D-mannose-6-phosphate, D-glucose-6-phosphate, ovalbumin and ovomucoid. Mannan inhibited the binding of bovine zona pellucida glycoproteins both to bull sperm and seminal plasma proteins. Yeast mannan immobilized to divinyl sulfone-activated Sepharose was used for the isolation of mannan-binding proteins. The protein components of this fraction were identified on the basis of relative molecular mass determination and N-terminal amino acid sequencing: RNAase dimer, PDC-109 and a protein homologous to BSP-30K (relative molecular mass 14,500). The isolated proteins were characterized by a high zona pellucida binding activity. (en)
- Pomocí testu ELBA byly studovány interakce proteinů semenné plasmy s mannanem. Vysoká vazebná aktivita na mannan byla pozorována u proteinové frakce reagující s heparinem. Vazba na mannan byla inhibována D-mannosou a D-fruktosou, ale neměl na ni vliv D-mannosa-6-fosfát, D-glukosa-6-fosfát, ovalbumin a ovomukoid. Mannan potlačoval vazbu glykoproteinů bovinní zony pellucidy na proteiny býčího spermatu a semenné plasmy. Kvasničný mannan imobilizovaný na divinylsufonem aktivovanou Sepharosu byl využit k izolaci proteinů vázajících mannan. Na základě určení relativní molekulové hmotnosti a sekvenování aminokyselinového N-konce byly identifikovány proteinové komponenty této frakce: dimer RNAsy, PDC-90 a protein homologni s BSP-30K (o relat. molek. hmostnosti 14 500). Izolované proteiny se vyznačovaly vysokou vazebnou aktivitou na zonu pellucidu. (cs)
|
| Title
| - Afinitní chromatografie býčích seminálních proteinů na mannan-Sepharose (cs)
- Affinity chromatography of bull seminal plasma proteins on mannan-Sepharose
- Affinity chromatography of bull seminal plasma proteins on mannan-Sepharose (en)
|
| skos:prefLabel
| - Afinitní chromatografie býčích seminálních proteinů na mannan-Sepharose (cs)
- Affinity chromatography of bull seminal plasma proteins on mannan-Sepharose
- Affinity chromatography of bull seminal plasma proteins on mannan-Sepharose (en)
|
| skos:notation
| - RIV/68378050:_____/02:00191436!RIV07-AV0-68378050
|
| http://linked.open.../vavai/riv/strany
| |
| http://linked.open...avai/riv/aktivita
| |
| http://linked.open...avai/riv/aktivity
| |
| http://linked.open...iv/cisloPeriodika
| |
| http://linked.open...vai/riv/dodaniDat
| |
| http://linked.open...aciTvurceVysledku
| |
| http://linked.open.../riv/druhVysledku
| |
| http://linked.open...iv/duvernostUdaju
| |
| http://linked.open...titaPredkladatele
| |
| http://linked.open...dnocenehoVysledku
| |
| http://linked.open...ai/riv/idVysledku
| - RIV/68378050:_____/02:00191436
|
| http://linked.open...riv/jazykVysledku
| |
| http://linked.open.../riv/klicovaSlova
| |
| http://linked.open.../riv/klicoveSlovo
| |
| http://linked.open...odStatuVydavatele
| |
| http://linked.open...ontrolniKodProRIV
| |
| http://linked.open...i/riv/nazevZdroje
| - Journal of Chromatography-B
|
| http://linked.open...in/vavai/riv/obor
| |
| http://linked.open...ichTvurcuVysledku
| |
| http://linked.open...cetTvurcuVysledku
| |
| http://linked.open...UplatneniVysledku
| |
| http://linked.open...v/svazekPeriodika
| |
| http://linked.open...iv/tvurceVysledku
| - Jonáková, Věra
- Ryšlavá, H.
- Liberda, J.
- Tichá, M.
- Jelínková, Petra
|
| http://linked.open...n/vavai/riv/zamer
| |
| issn
| |
| number of pages
| |
![[RDF Data]](/fct/images/sw-rdf-blue.png)
![[cxml]](/fct/images/cxml_doc.png)
![[csv]](/fct/images/csv_doc.png)
![[text]](/fct/images/ntriples_doc.png)
![[turtle]](/fct/images/n3turtle_doc.png)
![[ld+json]](/fct/images/jsonld_doc.png)
![[rdf+json]](/fct/images/json_doc.png)
![[rdf+xml]](/fct/images/xml_doc.png)
![[atom+xml]](/fct/images/atom_doc.png)
![[html]](/fct/images/html_doc.png)