About: 3D visualization of collagen in artificial scaffolds using two-photon excitation and second-harmonic generation imaging

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About: 3D visualization of collagen in artificial scaffolds using two-photon excitation and second-harmonic generation imaging Goto Sponge NotDistinct Permalink

An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Nonlinear microscopy can employ two different types of signals, fluorescence and second-harmonic generation (SHG), to image biological structures with subcellular resolution. SHG imaging was used for the identification of type II collagen in an extracellular matrix. Chondrocyte proliferation was linked to production of the extracellular matrix proteins. Collagen as a typical extracellular cartilage protein was visualized, first, by CLSM using fluorescently labeled monoclonal antibodies against type II collagen. Conventional confocal microscopy images were compared with the SHG imaging in both forward and backward nondescanned modes. Well detectable signals were observed in PCL and gelatine scaffolds. Detection of collagen by SHG imaging corresponded well with collagen detection by immunohistochemical staining Nonlinear microscopy can employ two different types of signals, fluorescence and second-harmonic generation (SHG), to image biological structures with subcellular resolution. SHG imaging was used for the identification of type II collagen in an extracellular matrix. Chondrocyte proliferation was linked to production of the extracellular matrix proteins. Collagen as a typical extracellular cartilage protein was visualized, first, by CLSM using fluorescently labeled monoclonal antibodies against type II collagen. Conventional confocal microscopy images were compared with the SHG imaging in both forward and backward nondescanned modes. Well detectable signals were observed in PCL and gelatine scaffolds. Detection of collagen by SHG imaging corresponded well with collagen detection by immunohistochemical staining (en)
Title	3D visualization of collagen in artificial scaffolds using two-photon excitation and second-harmonic generation imaging 3D visualization of collagen in artificial scaffolds using two-photon excitation and second-harmonic generation imaging (en)
skos:prefLabel	3D visualization of collagen in artificial scaffolds using two-photon excitation and second-harmonic generation imaging 3D visualization of collagen in artificial scaffolds using two-photon excitation and second-harmonic generation imaging (en)
skos:notation	RIV/68378041:_____/11:00373200!RIV12-AV0-68378041
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(7E09088), P(GA304/09/0733), P(GAP501/10/0340), P(IAA500390702), P(LC06063), P(MEB0810092), Z(AV0Z50110509), Z(AV0Z50390512), Z(MSM6840770012)
http://linked.open...vai/riv/dodaniDat	2012
http://linked.open...aciTvurceVysledku	Filová, Eva
http://linked.open.../riv/druhVysledku	O - Ostatní výsledky nezařaditelné do žádného z výše uvedených druhů výsledku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Ústav experimentální medicíny AV ČR, v. v. i.
http://linked.open...dnocenehoVysledku	244112
http://linked.open...ai/riv/idVysledku	RIV/68378041:_____/11:00373200
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	multi-photon microscopy; artificial scaffolds; collagen (en)
http://linked.open.../riv/klicoveSlovo	collagen artificial scaffolds multi-photon microscopy
http://linked.open...ontrolniKodProRIV	[1A1261B0B0CA]
http://linked.open...in/vavai/riv/obor	EA
http://linked.open...ichTvurcuVysledku	1 (xsd:int)
http://linked.open...cetTvurcuVysledku	5 (xsd:int)
http://linked.open...vavai/riv/projekt	Three-dimensional arrangement and remodelation of the capillary bed of placentas from pregnancies complicated by type I diabetes mellitus Flurescence microscopy in biological and medical research Impact of CO2 enrichment and irradiance on structure and performance of forest tree photosynthetic apparatus at different hierarchical levels Processing and analysis of three-dimensional biomedical images BIOactive highly porous and injectable Scaffolds controlling stem cell recruitment, proliferation and differentiation and enabling angiogenesis for Cardiovascular ENgineered Tissues Scaffolds from liposome nanofibers for tissue engineering
http://linked.open...UplatneniVysledku	2011
http://linked.open...iv/tvurceVysledku	Burdíková, Zuzana Janáček, Jiří Filová, Eva Čapek, M. Košťáková, E.
http://linked.open...n/vavai/riv/zamer	Výzkum molekulárních a buněčných základů fyziologických a patofyziologických procesů s cílem objasnit mechanismy vzniku závažných onemocnění člověka Molekulární, buněčné a systémové mechanizmy závažných onemocnění lidského organizmu, jejich diagnostika, terapie a farmakoterapie Transdisciplinární výzkum v oblasti biomedicínského inženýrství II

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