About: In vitro differentiation of mouse embryonic stem cells into neurons of the dorsal forebrain

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Pluripotent embryonic stem cells (ESCs) are able to differentiate into all cell types in the organism including neocortical neurons. To follow the dynamic generation of progenitors of the dorsal forebrain in vitro, we generated ESCs from D6-GFP mice in which GFP marks neocortical progenitors and neurons. Several differentiation protocols were used. GFP-positive neocorical cells were induced under differentiation conditions in quickly formed embryoid bodies (qEBs) after 12-day incubation. In contrast, protocols using normal embryoid bodies (nEBs) yielded only a few D6-GFP-positive cells. As shown by immunohistochemistry and qRT-PCR, neocortical cells generated from qEBs expressed characteristic genes such as Pax6, Dach1, Tbr1, Tbr2, or Sox5. Activation of Wnt signaling further stimulated generation of D6-GFP-positive cells. qEBs culture also allowed the formation of pseudo-polarized neuroepithelium with the apical N-cadherin and basal Pax6 that is typical of developing neocortex. Pluripotent embryonic stem cells (ESCs) are able to differentiate into all cell types in the organism including neocortical neurons. To follow the dynamic generation of progenitors of the dorsal forebrain in vitro, we generated ESCs from D6-GFP mice in which GFP marks neocortical progenitors and neurons. Several differentiation protocols were used. GFP-positive neocorical cells were induced under differentiation conditions in quickly formed embryoid bodies (qEBs) after 12-day incubation. In contrast, protocols using normal embryoid bodies (nEBs) yielded only a few D6-GFP-positive cells. As shown by immunohistochemistry and qRT-PCR, neocortical cells generated from qEBs expressed characteristic genes such as Pax6, Dach1, Tbr1, Tbr2, or Sox5. Activation of Wnt signaling further stimulated generation of D6-GFP-positive cells. qEBs culture also allowed the formation of pseudo-polarized neuroepithelium with the apical N-cadherin and basal Pax6 that is typical of developing neocortex. (en)
Title	In vitro differentiation of mouse embryonic stem cells into neurons of the dorsal forebrain In vitro differentiation of mouse embryonic stem cells into neurons of the dorsal forebrain (en)
skos:prefLabel	In vitro differentiation of mouse embryonic stem cells into neurons of the dorsal forebrain In vitro differentiation of mouse embryonic stem cells into neurons of the dorsal forebrain (en)
skos:notation	RIV/68378041:_____/11:00370061!RIV12-AV0-68378041
http://linked.open...avai/riv/aktivita	I P Z
http://linked.open...avai/riv/aktivity	I, P(GA204/08/1618), Z(AV0Z50390512), Z(AV0Z50520514)
http://linked.open...iv/cisloPeriodika	5
http://linked.open...vai/riv/dodaniDat	2012
http://linked.open...aciTvurceVysledku	Hampl, Aleš
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Ústav experimentální medicíny AV ČR, v. v. i.
http://linked.open...dnocenehoVysledku	204035
http://linked.open...ai/riv/idVysledku	RIV/68378041:_____/11:00370061
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	embryonic stem cells; differentiation; neocortex (en)
http://linked.open.../riv/klicoveSlovo	embryonic stem cells neocortex differentiation
http://linked.open...odStatuVydavatele	US - Spojené státy americké
http://linked.open...ontrolniKodProRIV	[F90535B4EBAF]
http://linked.open...i/riv/nazevZdroje	Cellular and Molecular Neurobiology
http://linked.open...in/vavai/riv/obor	EB
http://linked.open...ichTvurcuVysledku	1 (xsd:int)
http://linked.open...cetTvurcuVysledku	6 (xsd:int)
http://linked.open...vavai/riv/projekt	Molecular basis of canonical Wnt signalling during eye and brain development
http://linked.open...UplatneniVysledku	2011
http://linked.open...v/svazekPeriodika	31
http://linked.open...iv/tvurceVysledku	Hampl, Aleš Dvořák, P. Jing, Y. Krauss, S. Machoň, Ondřej Xing, Y.
http://linked.open...ain/vavai/riv/wos	000290914300008
http://linked.open...n/vavai/riv/zamer	Molekulární, buněčné a systémové mechanizmy závažných onemocnění lidského organizmu, jejich diagnostika, terapie a farmakoterapie Molekulárně genetické a buněčné základy klíčových biologických procesů: genová exprese, onkogeneze, replikace virů, imunita a vývoj organismů
issn	0272-4340
number of pages	13 (xsd:int)
http://bibframe.org/vocab/doi	10.1007/s10571-011-9669-2

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