About: Raster image correlation spectroscopy as a novel tool to study interactions of macromolecules with nanofiber scaffolds

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About: Raster image correlation spectroscopy as a novel tool to study interactions of macromolecules with nanofiber scaffolds Goto Sponge NotDistinct Permalink

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Dynamic processes such as diffusion and binding/unbinding of macromolecules (e.g. growth factors or nutrients) are crucial parameters for the design and application of effective artificial tissue materials. Here, dynamics of selected macromolecules were studied in two different composite tissue engineering scaffolds containing an electrospun nanofiber mesh (polycaprolactone or hydrophobically plasma modified polyvinylalcohol–chitosan) encapsulated in agarose hydrogels by a conventional approach fluorescence recovery after photobleaching (FRAP) and a novel technique, raster image correlation spectroscopy (RICS). The two approaches are compared, and it is shown that FRAP is unable to determine processes occurring at low molecular concentrations, especially accurately separating binding/unbinding from diffusion, and its results depend on the concentration of the studied molecules. Dynamic processes such as diffusion and binding/unbinding of macromolecules (e.g. growth factors or nutrients) are crucial parameters for the design and application of effective artificial tissue materials. Here, dynamics of selected macromolecules were studied in two different composite tissue engineering scaffolds containing an electrospun nanofiber mesh (polycaprolactone or hydrophobically plasma modified polyvinylalcohol–chitosan) encapsulated in agarose hydrogels by a conventional approach fluorescence recovery after photobleaching (FRAP) and a novel technique, raster image correlation spectroscopy (RICS). The two approaches are compared, and it is shown that FRAP is unable to determine processes occurring at low molecular concentrations, especially accurately separating binding/unbinding from diffusion, and its results depend on the concentration of the studied molecules. (en)
Title	Raster image correlation spectroscopy as a novel tool to study interactions of macromolecules with nanofiber scaffolds Raster image correlation spectroscopy as a novel tool to study interactions of macromolecules with nanofiber scaffolds (en)
skos:prefLabel	Raster image correlation spectroscopy as a novel tool to study interactions of macromolecules with nanofiber scaffolds Raster image correlation spectroscopy as a novel tool to study interactions of macromolecules with nanofiber scaffolds (en)
skos:notation	RIV/68378041:_____/11:00368065!RIV12-AV0-68378041
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(GA202/09/1151), P(GAP304/10/1307), P(IAA500390702), P(LC06063), Z(AV0Z40400503), Z(AV0Z50390512), Z(AV0Z50390703), Z(AV0Z50520514)
http://linked.open...iv/cisloPeriodika	12
http://linked.open...vai/riv/dodaniDat	2012
http://linked.open...aciTvurceVysledku	Amler, Evžen Buzgo, Matej
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Ústav experimentální medicíny AV ČR, v. v. i.
http://linked.open...dnocenehoVysledku	225727
http://linked.open...ai/riv/idVysledku	RIV/68378041:_____/11:00368065
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	fluorecence dynamics; transient binding; diffusion (en)
http://linked.open.../riv/klicoveSlovo	diffusion fluorecence dynamics transient binding
http://linked.open...odStatuVydavatele	GB - Spojené království Velké Británie a Severního Irska
http://linked.open...ontrolniKodProRIV	[50FF43BA0143]
http://linked.open...i/riv/nazevZdroje	Acta Biomaterialia
http://linked.open...in/vavai/riv/obor	CF
http://linked.open...ichTvurcuVysledku	2 (xsd:int)
http://linked.open...cetTvurcuVysledku	8 (xsd:int)
http://linked.open...vavai/riv/projekt	Flurescence microscopy in biological and medical research Focused tandem shock waves and their potential application in cancer treatment and controlled drug delivery Smart nanofiber/liposme composite scaffold for bone regeneration Scaffolds from liposome nanofibers for tissue engineering
http://linked.open...UplatneniVysledku	2011
http://linked.open...v/svazekPeriodika	7
http://linked.open...iv/tvurceVysledku	Hof, Martin Humpolíčková, Jana Macháň, Radek Amler, Evžen Buzgo, Matej Huranová, Martina Lukáš, D. Norris, S. C. P.
http://linked.open...ain/vavai/riv/wos	000297436500012
http://linked.open...n/vavai/riv/zamer	Struktura, reaktivita a dynamika molekulárních a biomolekulárních systémů: teorie, experiment, aplikace Molekulární, buněčné a systémové mechanizmy závažných onemocnění lidského organizmu, jejich diagnostika, terapie a farmakoterapie New biotechnologies, nanomaterials and stem cells for use in regenerative medicine Molekulárně genetické a buněčné základy klíčových biologických procesů: genová exprese, onkogeneze, replikace virů, imunita a vývoj organismů
issn	1742-7061
number of pages	9 (xsd:int)
http://bibframe.org/vocab/doi	10.1016/j.actbio.2011.07.012

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