About: Molecular differentiation of three loach species (Pisces, Cobitidae) based on the nuclear 5S rDNA marker

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About: Molecular differentiation of three loach species (Pisces, Cobitidae) based on the nuclear 5S rDNA marker Goto Sponge NotDistinct Permalink

An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	The diversity of the 5S rDNA fragment among three loach species: Cobitis taenia, C. elongatoides and Sabanejewia aurata was investigated using universal PCR primers for this gene. Three amplification products were obtained: 220 bp length for C. taenia and C. elongatoides, and 330 bp for S. aurata. Two amplicons with the same length (in Cobitis) were digested with TaqI restriction endonuclease. This enzyme found one restriction site T/CGA in the C. elongatoides fragment, while in the case of C. taenia no cleavage effect was observed. On this basis we constructed an easy and cheap method for loach species discrimination. It seems adequate for effective support of conservation initiatives for endangered loathes. The diversity of the 5S rDNA fragment among three loach species: Cobitis taenia, C. elongatoides and Sabanejewia aurata was investigated using universal PCR primers for this gene. Three amplification products were obtained: 220 bp length for C. taenia and C. elongatoides, and 330 bp for S. aurata. Two amplicons with the same length (in Cobitis) were digested with TaqI restriction endonuclease. This enzyme found one restriction site T/CGA in the C. elongatoides fragment, while in the case of C. taenia no cleavage effect was observed. On this basis we constructed an easy and cheap method for loach species discrimination. It seems adequate for effective support of conservation initiatives for endangered loathes. (en)
Title	Molecular differentiation of three loach species (Pisces, Cobitidae) based on the nuclear 5S rDNA marker Molecular differentiation of three loach species (Pisces, Cobitidae) based on the nuclear 5S rDNA marker (en)
skos:prefLabel	Molecular differentiation of three loach species (Pisces, Cobitidae) based on the nuclear 5S rDNA marker Molecular differentiation of three loach species (Pisces, Cobitidae) based on the nuclear 5S rDNA marker (en)
skos:notation	RIV/68081766:_____/11:00384520!RIV13-AV0-68081766
http://linked.open...avai/riv/aktivita	Z
http://linked.open...avai/riv/aktivity	Z(AV0Z60930519)
http://linked.open...iv/cisloPeriodika	3-4
http://linked.open...vai/riv/dodaniDat	2013
http://linked.open...aciTvurceVysledku	Lusková, Věra Lusk, Stanislav
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Ústav biologie obratlovců AV ČR, v. v. i.
http://linked.open...dnocenehoVysledku	213488
http://linked.open...ai/riv/idVysledku	RIV/68081766:_____/11:00384520
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Cobitis; PCR-RFLP; Sabanejewia; species identification; 5S rDNA (en)
http://linked.open.../riv/klicoveSlovo	5S rDNA Cobitis Sabanejewia species identification PCR-RFLP
http://linked.open...odStatuVydavatele	PL - Polská republika
http://linked.open...ontrolniKodProRIV	[2FF76AFAB970]
http://linked.open...i/riv/nazevZdroje	Folia Biologica-Krakow
http://linked.open...in/vavai/riv/obor	EB
http://linked.open...ichTvurcuVysledku	2 (xsd:int)
http://linked.open...cetTvurcuVysledku	5 (xsd:int)
http://linked.open...UplatneniVysledku	2011
http://linked.open...v/svazekPeriodika	59
http://linked.open...iv/tvurceVysledku	Lusk, Stanislav Lusková, Věra Boron, A. Kirtiklis, L. Ptasznik, P.
http://linked.open...ain/vavai/riv/wos	000293365100009
http://linked.open...n/vavai/riv/zamer	Biodiverzita a ekologie obratlovců: Strategie ochrany a využívání přírodních populací
issn	0015-5497
number of pages	5 (xsd:int)
http://bibframe.org/vocab/doi	10.3409/fb59_3-4.141-145

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