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| rdf:type
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| Description
| - Aim of this study was to evaluate cellular fatty acid analysis for characterization of spirochetes. Strains were isolated from arthropods collected in South Moravia, Czech Republic. Fatty acid methyl esters (FAME) profile was determined for five Borrelia burgdorferi sensu lato (s.l.) strains isolated from Ixodes ricinus ticks, one %22Spironema culicis%22 strain recovered from mosquito Culex pipiens and seven spirochetal strains (not identified yet) isolated from mosquitoes and blackflies. Analysis was performed using a gas chromatography column in conjunction with Microbial Identification System Sherlock (MIDI Inc., Newark, DE, USA). Results obtained on the basis of cluster analysis of FAME profiles showed, that the B. burgdorferi sensu lato isolates could be well separated from other spirochetal isolates. We recommended method used in this study as a useful tool for preliminary identification of spirochetes isolated from ticks and dipterans.
- Aim of this study was to evaluate cellular fatty acid analysis for characterization of spirochetes. Strains were isolated from arthropods collected in South Moravia, Czech Republic. Fatty acid methyl esters (FAME) profile was determined for five Borrelia burgdorferi sensu lato (s.l.) strains isolated from Ixodes ricinus ticks, one %22Spironema culicis%22 strain recovered from mosquito Culex pipiens and seven spirochetal strains (not identified yet) isolated from mosquitoes and blackflies. Analysis was performed using a gas chromatography column in conjunction with Microbial Identification System Sherlock (MIDI Inc., Newark, DE, USA). Results obtained on the basis of cluster analysis of FAME profiles showed, that the B. burgdorferi sensu lato isolates could be well separated from other spirochetal isolates. We recommended method used in this study as a useful tool for preliminary identification of spirochetes isolated from ticks and dipterans. (en)
- Cílem studie bylo posoudit využití analýzy mastných kyselin k charakterizaci spirochét. Testované kmeny byly izolovány z členovců odchycených na jižní Moravě. Profil mastných kyselin ve formě jejich methylesterů (FAME) byl stanoven pro 5 kmenů Borrelia burgdorferi sensu lato (s.l.) izolovaných z klíšťat Ixodes ricinus, kmen %22Spironema culicis%22 získaný z komára Culex pipiens a sedm kmenů dosud oficiálně nezařazených spirochét izolovaných z komárů a muchniček. Analýza byla provedena na koloně plynového chromatografu za použití mikrobiálního identifikačního systému (MIS) Sherlock (MIDI Inc., Newark, DE, USA). Výsledky získané na základě klastrové analýzy FAME profilů ukázaly, že izoláty B. burgdorferi (s.l.) lze dobře odlišit od spirochét izolovaných z krevsajícího hmyzu. Zvolenou metodu lze s použitím identifikačního systému MIS Sherlock doporučit jako užitečný nástroj předběžné identifikace spirochét izolovaných z klíšťat a z dvojkřídlého hmyzu. (cs)
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| Title
| - Characterization of spirochetal isolates from arthropods collected in South Moravia, Czech Republic, using fatty acid methyl esters analysis
- Charakterizace spirochét izolovaných z členovců na jižní Moravě, Česká republika, analýzou methylesterů mastných kyselin (cs)
- Characterization of spirochetal isolates from arthropods collected in South Moravia, Czech Republic, using fatty acid methyl esters analysis (en)
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| skos:prefLabel
| - Characterization of spirochetal isolates from arthropods collected in South Moravia, Czech Republic, using fatty acid methyl esters analysis
- Charakterizace spirochét izolovaných z členovců na jižní Moravě, Česká republika, analýzou methylesterů mastných kyselin (cs)
- Characterization of spirochetal isolates from arthropods collected in South Moravia, Czech Republic, using fatty acid methyl esters analysis (en)
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| skos:notation
| - RIV/68081766:_____/04:00101771!RIV/2005/GA0/A67005/N
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| http://linked.open.../vavai/riv/strany
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| http://linked.open...avai/riv/aktivita
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| http://linked.open...avai/riv/aktivity
| - P(GA206/03/0726), Z(AV0Z6093917), Z(MZ06462829901)
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| http://linked.open...iv/cisloPeriodika
| |
| http://linked.open...vai/riv/dodaniDat
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| http://linked.open...aciTvurceVysledku
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| http://linked.open.../riv/druhVysledku
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| http://linked.open...iv/duvernostUdaju
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| http://linked.open...titaPredkladatele
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| http://linked.open...dnocenehoVysledku
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| http://linked.open...ai/riv/idVysledku
| - RIV/68081766:_____/04:00101771
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| http://linked.open...riv/jazykVysledku
| |
| http://linked.open.../riv/klicovaSlova
| - spirochetes;arthropods;fatty acid methyl esters (en)
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| http://linked.open.../riv/klicoveSlovo
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| http://linked.open...odStatuVydavatele
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| http://linked.open...ontrolniKodProRIV
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| http://linked.open...i/riv/nazevZdroje
| - Journal of Chromatography B
|
| http://linked.open...in/vavai/riv/obor
| |
| http://linked.open...ichTvurcuVysledku
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| http://linked.open...cetTvurcuVysledku
| |
| http://linked.open...vavai/riv/projekt
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| http://linked.open...UplatneniVysledku
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| http://linked.open...v/svazekPeriodika
| |
| http://linked.open...iv/tvurceVysledku
| - Halouzka, Jiří
- Šikutová, Silvie
- Němec, M.
- Čechová, L.
- Durnová, E.
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| http://linked.open...n/vavai/riv/zamer
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| issn
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| number of pages
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