About: Comparison of assays for the detection of West Nile virus antibodies in chicken serum.

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About: Comparison of assays for the detection of West Nile virus antibodies in chicken serum. Goto Sponge NotDistinct Permalink

An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Six tests for the detection of West Nile virus (WNV) antibodies in the serum of experimentally infected chickens were compared. The tests included the hemagglutination-inhibition test (HIT), immunoglobulin M (IgM)-capture enzyme-linked immunosorbent assay (ELISA) with WNV-infected mouse brain antigen, immunoglobulin G (IgG) indirect ELISA with tickborne encephalitis viral antigen, the microtitre virus neutralization test, the standard plaque reduction neutralization test (PRNT), and the microtitre PRNT (micro-PRNT). Thirty adult chickens, intravenously and intramuscularly inoculated with 101 plaque-forming units (PFU) of WNV strain Egypt 101, were bled and given a booster of 10(7) PFU at 7,15, and 21 d postinoculation; the final blood collection was on day 28. Although the micro-PRNT is capable of detecting the highest antibody titres during both early and late infection, because of the technical complexity and time requirements of this test a combination of IgM and IgG ELISAs is recommended for serol Six tests for the detection of West Nile virus (WNV) antibodies in the serum of experimentally infected chickens were compared. The tests included the hemagglutination-inhibition test (HIT), immunoglobulin M (IgM)-capture enzyme-linked immunosorbent assay (ELISA) with WNV-infected mouse brain antigen, immunoglobulin G (IgG) indirect ELISA with tickborne encephalitis viral antigen, the microtitre virus neutralization test, the standard plaque reduction neutralization test (PRNT), and the microtitre PRNT (micro-PRNT). Thirty adult chickens, intravenously and intramuscularly inoculated with 101 plaque-forming units (PFU) of WNV strain Egypt 101, were bled and given a booster of 10(7) PFU at 7,15, and 21 d postinoculation; the final blood collection was on day 28. Although the micro-PRNT is capable of detecting the highest antibody titres during both early and late infection, because of the technical complexity and time requirements of this test a combination of IgM and IgG ELISAs is recommended for serol (en)
Title	Comparison of assays for the detection of West Nile virus antibodies in chicken serum. Comparison of assays for the detection of West Nile virus antibodies in chicken serum. (en)
skos:prefLabel	Comparison of assays for the detection of West Nile virus antibodies in chicken serum. Comparison of assays for the detection of West Nile virus antibodies in chicken serum. (en)
skos:notation	RIV/68081766:_____/03:67030031!RIV/2004/AV0/A67004/N
http://linked.open.../vavai/riv/strany	128;132
http://linked.open...avai/riv/aktivita	Z
http://linked.open...avai/riv/aktivity	Z(AV0Z6093917)
http://linked.open...iv/cisloPeriodika	2
http://linked.open...vai/riv/dodaniDat	2004
http://linked.open...aciTvurceVysledku	Hubálek, Zdeněk Halouzka, Jiří
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Ústav biologie obratlovců AV ČR, v. v. i.
http://linked.open...dnocenehoVysledku	601683
http://linked.open...ai/riv/idVysledku	RIV/68081766:_____/03:67030031
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	West Nile virus (en)
http://linked.open.../riv/klicoveSlovo	West Nile virus
http://linked.open...odStatuVydavatele	CA - Kanada
http://linked.open...ontrolniKodProRIV	[502648ACF61E]
http://linked.open...i/riv/nazevZdroje	Canadian journal of veterinary research
http://linked.open...in/vavai/riv/obor	GJ
http://linked.open...ichTvurcuVysledku	2 (xsd:int)
http://linked.open...cetTvurcuVysledku	9 (xsd:int)
http://linked.open...ocetUcastnikuAkce	0 (xsd:int)
http://linked.open...nichUcastnikuAkce	0 (xsd:int)
http://linked.open...UplatneniVysledku	2003
http://linked.open...v/svazekPeriodika	67
http://linked.open...iv/tvurceVysledku	Hubálek, Zdeněk Halouzka, Jiří Andonova, M. Cottam-Birt, C. Dibernardo, A. Drebot, M. A. Larence, J. Marszal, P. Weingartl, H. M.
http://linked.open...n/vavai/riv/zamer	Diversity and life history of free-living vertebrates and implication of results in the strategy of conservation and sustainable management of natural resources
issn	0830-9000
number of pages	5 (xsd:int)

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