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  • Super-paramagnetic particles are used extensively in diagnostics and other research applications for the purification of cells, biomolecules and assays. Here, we demonstrate full 3D optical manipulation of such sub-micrometer sized particle using optical tweezers. We report three types of anomalous behavior of such a trapped particle. Firstly, the analysis of particle motion recorded by the quadrant photodiode reveals spikes in the total detected intensity that are formed by a set of very fast oscillations. Secondly, the dependence of trap stiffness on the laser power deviates from the expected linear increase for higher trapping powers. And further, such a particle cannot be optically trapped above certain trapping power threshold. We speculate that these effects are caused by particle internal structure and by its heating.
  • Super-paramagnetic particles are used extensively in diagnostics and other research applications for the purification of cells, biomolecules and assays. Here, we demonstrate full 3D optical manipulation of such sub-micrometer sized particle using optical tweezers. We report three types of anomalous behavior of such a trapped particle. Firstly, the analysis of particle motion recorded by the quadrant photodiode reveals spikes in the total detected intensity that are formed by a set of very fast oscillations. Secondly, the dependence of trap stiffness on the laser power deviates from the expected linear increase for higher trapping powers. And further, such a particle cannot be optically trapped above certain trapping power threshold. We speculate that these effects are caused by particle internal structure and by its heating. (en)
Title
  • Anomalous behavior of a three-dimensional, optically trapped, super-paramagnetic particle
  • Anomalous behavior of a three-dimensional, optically trapped, super-paramagnetic particle (en)
skos:prefLabel
  • Anomalous behavior of a three-dimensional, optically trapped, super-paramagnetic particle
  • Anomalous behavior of a three-dimensional, optically trapped, super-paramagnetic particle (en)
skos:notation
  • RIV/68081731:_____/14:00434983!RIV15-GA0-68081731
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  • I, P(ED0017/01/01), P(GPP205/12/P868), P(LO1212)
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  • 3333
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  • RIV/68081731:_____/14:00434983
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  • Particles; Photodiodes; Diagnostics; Lasers; Optical manipulation; Optical tweezers (en)
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http://linked.open...ontrolniKodProRIV
  • [CDAD4986DD92]
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  • San Diego
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  • Bellingham
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  • Optical Trapping and Optical Micromanipulation XI (Proceedings of SPIE 9164)
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  • Brzobohatý, Oto
  • Ježek, Jan
  • Zemánek, Pavel
  • Jákl, Petr
  • Šiler, Martin
http://linked.open...vavai/riv/typAkce
http://linked.open.../riv/zahajeniAkce
issn
  • 0277-786X
number of pages
http://bibframe.org/vocab/doi
  • 10.1117/12.2062148
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  • SPIE
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  • 9781628411911
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