About: Raman tweezers in microfluidic systems for analysis and sorting of living cells

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An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	We have devised an analytical and sorting system combining optical trapping with Raman spectroscopy in microfluidic environment in order to identify and sort biological objects, such as living cells of various prokaryotic and eukaryotic organisms. Our main objective was to create a robust and universal platform for non-contact sorting of microobjects based on their Raman spectral properties. This approach allowed us to collect information about the chemical composition of the objects, such as the presence and composition of lipids, proteins, or nucleic acids without using artificial chemical probes such as fluorescent markers. The non-destructive and non-contact nature of this optical analysis and manipulation allowed us to separate individual living cells of our interest in a sterile environment and provided the possibility to cultivate the selected cells for further experiments. We used differently treated cells of algae to test and demonstrate the function of our analytical and sorting system. The devised system could find its use in many medical, biotechnological, and biological applications. We have devised an analytical and sorting system combining optical trapping with Raman spectroscopy in microfluidic environment in order to identify and sort biological objects, such as living cells of various prokaryotic and eukaryotic organisms. Our main objective was to create a robust and universal platform for non-contact sorting of microobjects based on their Raman spectral properties. This approach allowed us to collect information about the chemical composition of the objects, such as the presence and composition of lipids, proteins, or nucleic acids without using artificial chemical probes such as fluorescent markers. The non-destructive and non-contact nature of this optical analysis and manipulation allowed us to separate individual living cells of our interest in a sterile environment and provided the possibility to cultivate the selected cells for further experiments. We used differently treated cells of algae to test and demonstrate the function of our analytical and sorting system. The devised system could find its use in many medical, biotechnological, and biological applications. (en)
Title	Raman tweezers in microfluidic systems for analysis and sorting of living cells Raman tweezers in microfluidic systems for analysis and sorting of living cells (en)
skos:prefLabel	Raman tweezers in microfluidic systems for analysis and sorting of living cells Raman tweezers in microfluidic systems for analysis and sorting of living cells (en)
skos:notation	RIV/68081731:_____/14:00434961!RIV15-TA0-68081731
http://linked.open...avai/riv/aktivita	I P
http://linked.open...avai/riv/aktivity	I, P(ED0017/01/01), P(FR-TI1/433), P(LO1212), P(TA03010642)
http://linked.open...vai/riv/dodaniDat	2015
http://linked.open...aciTvurceVysledku	Ježek, Jan Zemánek, Pavel Pilát, Zdeněk Kaňka, Jan
http://linked.open.../riv/druhVysledku	D - Článek ve sborníku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Ústav přístrojové techniky AV ČR, v. v. i.
http://linked.open...dnocenehoVysledku	41276
http://linked.open...ai/riv/idVysledku	RIV/68081731:_____/14:00434961
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Chemicals; Fluorescent markers; Optical analysis; Optical trapping; Organisms; Raman spectroscopy (en)
http://linked.open.../riv/klicoveSlovo	Raman spectroscopy Optical trapping Chemicals Fluorescent markers Optical analysis Organisms
http://linked.open...ontrolniKodProRIV	[04AA8F1245E0]
http://linked.open...v/mistoKonaniAkce	San Francisco
http://linked.open...i/riv/mistoVydani	Bellingham
http://linked.open...i/riv/nazevZdroje	Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XII (Proceedings of SPIE 8947)
http://linked.open...in/vavai/riv/obor	BH
http://linked.open...ichTvurcuVysledku	4 (xsd:int)
http://linked.open...cetTvurcuVysledku	4 (xsd:int)
http://linked.open...vavai/riv/projekt	ALISI - Centre of advanced diagnostic methods and technologies APPLICATION LABORATORIES OF ADVANCED MICROTECHNOLOGIES AND NANOTECHNOLOGIES *Development of instrumentation and methodology for the selection of photoautotrophic microorganisms for production of higher-generation biofuels Advanced microfluidic techniques
http://linked.open...UplatneniVysledku	2014
http://linked.open...iv/tvurceVysledku	Ježek, Jan Zemánek, Pavel Kaňka, Jan Pilát, Zdeněk
http://linked.open...vavai/riv/typAkce	WRD - Světová
http://linked.open...ain/vavai/riv/wos	000336037200037
http://linked.open.../riv/zahajeniAkce	2014-02-03 (xsd:date)
issn	1605-7422
number of pages	9 (xsd:int)
http://bibframe.org/vocab/doi	10.1117/12.2040631
http://purl.org/ne...btex#hasPublisher	SPIE
https://schema.org/isbn	9780819498601

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