About: Raman spectroscopy for bacterial identification and characterization

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	The main goal of our investigation is to use Raman tweezers technique so that the responce of Raman scattering on microorganisms suspended in liquid media (bacteria, algae and yeast cells in microfluidic chips) can be used to identify different species. The investigations presented here include identification of different bacteria strains (biofilm-positive and biofilm-negative) and yeast cells by using principal component analysis (PCA). The main driving force behind our investigation was a common problem in the clinical microbiology laboratory - how to distinguish between contaminant and invasive isolates. Invasive bacterial/yeast isolates can be assumed to form a biofilm, while isolates which do not form a biofilm can be treated as contaminant. Thus, the latter do not represent an important virulence factor. The main goal of our investigation is to use Raman tweezers technique so that the responce of Raman scattering on microorganisms suspended in liquid media (bacteria, algae and yeast cells in microfluidic chips) can be used to identify different species. The investigations presented here include identification of different bacteria strains (biofilm-positive and biofilm-negative) and yeast cells by using principal component analysis (PCA). The main driving force behind our investigation was a common problem in the clinical microbiology laboratory - how to distinguish between contaminant and invasive isolates. Invasive bacterial/yeast isolates can be assumed to form a biofilm, while isolates which do not form a biofilm can be treated as contaminant. Thus, the latter do not represent an important virulence factor. (en)
Title	Raman spectroscopy for bacterial identification and characterization Raman spectroscopy for bacterial identification and characterization (en)
skos:prefLabel	Raman spectroscopy for bacterial identification and characterization Raman spectroscopy for bacterial identification and characterization (en)
skos:notation	RIV/68081731:_____/12:00388104!RIV13-GA0-68081731
http://linked.open...avai/riv/aktivita	I P
http://linked.open...avai/riv/aktivity	I, P(ED0017/01/01), P(EE2.4.31.0016), P(GAP205/11/1687)
http://linked.open...vai/riv/dodaniDat	2013
http://linked.open...aciTvurceVysledku	Ježek, Jan Zemánek, Pavel Samek, Ota Šerý, Mojmír Bernatová, Silvie Pilát, Zdeněk Krzyžánek, Vladislav
http://linked.open.../riv/druhVysledku	D - Článek ve sborníku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Ústav přístrojové techniky AV ČR, v. v. i.
http://linked.open...dnocenehoVysledku	164027
http://linked.open...ai/riv/idVysledku	RIV/68081731:_____/12:00388104
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Raman spectroscopy; Raman tweezers; bacteria; yeast biofilm; PCA (en)
http://linked.open.../riv/klicoveSlovo	PCA Raman spectroscopy bacteria yeast biofilm Raman tweezers
http://linked.open...ontrolniKodProRIV	[B645D1B8403C]
http://linked.open...v/mistoKonaniAkce	Ostravice
http://linked.open...i/riv/mistoVydani	Bellingham
http://linked.open...i/riv/nazevZdroje	CPS 2012. 18th Czech-Polish-Slovak Optical Conference on Wave and Quantum Aspects of Contemporary Optics (Proceedings of SPIE Vol.8697)
http://linked.open...in/vavai/riv/obor	BH
http://linked.open...ichTvurcuVysledku	7 (xsd:int)
http://linked.open...cetTvurcuVysledku	8 (xsd:int)
http://linked.open...vavai/riv/projekt	APPLICATION LABORATORIES OF ADVANCED MICROTECHNOLOGIES AND NANOTECHNOLOGIES MEDTECH - educational and research partner network in medicine, biomedicine and scientific instruments Non-invasive contactless methods of identification and characterization of living microorganisms by optical spectroscopy and micromanipulation
http://linked.open...UplatneniVysledku	2012
http://linked.open...iv/tvurceVysledku	Ježek, Jan Samek, Ota Zemánek, Pavel Bernatová, Silvie Šerý, Mojmír Pilát, Zdeněk Růžička, F. Krzyžánek, Vladislav
http://linked.open...vavai/riv/typAkce	EUR - Evropská
http://linked.open.../riv/zahajeniAkce	2012-09-03 (xsd:date)
number of pages	6 (xsd:int)
http://bibframe.org/vocab/doi	10.1117/12.2010539
http://purl.org/ne...btex#hasPublisher	SPIE
https://schema.org/isbn	978-0-8194-9481-8

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