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Description
  • We have constructed a device for active optical manipulation and Raman spectral analysis in a microfluidic channel for efficient, nondestructive and contactless sorting of biological samples based on the Raman spectroscopic characteristics of living cells. In our previous work, we have linked such Raman spectral characteristics of microalgal lipid bodies with the unsaturation or carotene concentration via a calibration curve. As the sorting platform we have used a combination of fast galvano-optic laser steering system and specially designed microfluidic chips. We used X shaped channels with two input and output ports, and also several differently shaped variants. The steerable trapping laser beam was designed to move the cell to the specified locations and confine the cell for the time period needed for the analysis.
  • We have constructed a device for active optical manipulation and Raman spectral analysis in a microfluidic channel for efficient, nondestructive and contactless sorting of biological samples based on the Raman spectroscopic characteristics of living cells. In our previous work, we have linked such Raman spectral characteristics of microalgal lipid bodies with the unsaturation or carotene concentration via a calibration curve. As the sorting platform we have used a combination of fast galvano-optic laser steering system and specially designed microfluidic chips. We used X shaped channels with two input and output ports, and also several differently shaped variants. The steerable trapping laser beam was designed to move the cell to the specified locations and confine the cell for the time period needed for the analysis. (en)
Title
  • Microfluidic systems for optical sorting
  • Microfluidic systems for optical sorting (en)
skos:prefLabel
  • Microfluidic systems for optical sorting
  • Microfluidic systems for optical sorting (en)
skos:notation
  • RIV/68081731:_____/12:00388103!RIV13-GA0-68081731
http://linked.open...avai/riv/aktivita
http://linked.open...avai/riv/aktivity
  • I, P(ED0017/01/01), P(FR-TI1/433), P(GAP205/11/1687)
http://linked.open...vai/riv/dodaniDat
http://linked.open...aciTvurceVysledku
http://linked.open.../riv/druhVysledku
http://linked.open...iv/duvernostUdaju
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http://linked.open...dnocenehoVysledku
  • 150339
http://linked.open...ai/riv/idVysledku
  • RIV/68081731:_____/12:00388103
http://linked.open...riv/jazykVysledku
http://linked.open.../riv/klicovaSlova
  • microfluidic; cell sorting; optical tweezers; Raman spectroscopy (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...ontrolniKodProRIV
  • [4A859F6FBCEE]
http://linked.open...v/mistoKonaniAkce
  • Ostravice
http://linked.open...i/riv/mistoVydani
  • Bellingham
http://linked.open...i/riv/nazevZdroje
  • CPS 2012. 18th Czech-Polish-Slovak Optical Conference on Wave and Quantum Aspects of Contemporary Optics (Proceedings of SPIE Vol.8697)
http://linked.open...in/vavai/riv/obor
http://linked.open...ichTvurcuVysledku
http://linked.open...cetTvurcuVysledku
http://linked.open...vavai/riv/projekt
http://linked.open...UplatneniVysledku
http://linked.open...iv/tvurceVysledku
  • Ježek, Jan
  • Samek, Ota
  • Zemánek, Pavel
  • Kaňka, Jan
  • Bernatová, Silvie
  • Šerý, Mojmír
  • Pilát, Zdeněk
http://linked.open...vavai/riv/typAkce
http://linked.open.../riv/zahajeniAkce
number of pages
http://bibframe.org/vocab/doi
  • 10.1117/12.2008649
http://purl.org/ne...btex#hasPublisher
  • SPIE
https://schema.org/isbn
  • 978-0-8194-9481-8
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