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  • This article describes the usage of non-commercial environmental scanning electron microscope (ESEM) for the visualization of plant extracellular matrix in Abies alba and Abies numidica. Non sputter-coated samples free of using the common fixation technique observed at the relatively low humidity of the air environment with the pressure 550 Pa and the low temperature of the sample from -18 to -22A degrees C give surprisingly very good results that show the natural structure of the tissues. This seems to be generally applicable. Moreover, a specially designed ionization detector of secondary electrons and a YAG:Ce3+ detector of backscattered electrons were used for better comparison.
  • This article describes the usage of non-commercial environmental scanning electron microscope (ESEM) for the visualization of plant extracellular matrix in Abies alba and Abies numidica. Non sputter-coated samples free of using the common fixation technique observed at the relatively low humidity of the air environment with the pressure 550 Pa and the low temperature of the sample from -18 to -22A degrees C give surprisingly very good results that show the natural structure of the tissues. This seems to be generally applicable. Moreover, a specially designed ionization detector of secondary electrons and a YAG:Ce3+ detector of backscattered electrons were used for better comparison. (en)
Title
  • Imaging of early conifer embryogenic tissues with the environmental scanning electron microscope
  • Imaging of early conifer embryogenic tissues with the environmental scanning electron microscope (en)
skos:prefLabel
  • Imaging of early conifer embryogenic tissues with the environmental scanning electron microscope
  • Imaging of early conifer embryogenic tissues with the environmental scanning electron microscope (en)
skos:notation
  • RIV/68081731:_____/12:00385176!RIV13-GA0-68081731
http://linked.open...avai/riv/aktivita
http://linked.open...avai/riv/aktivity
  • I, P(GAP102/10/1410)
http://linked.open...iv/cisloPeriodika
  • 3
http://linked.open...vai/riv/dodaniDat
http://linked.open...aciTvurceVysledku
http://linked.open.../riv/druhVysledku
http://linked.open...iv/duvernostUdaju
http://linked.open...titaPredkladatele
http://linked.open...dnocenehoVysledku
  • 140513
http://linked.open...ai/riv/idVysledku
  • RIV/68081731:_____/12:00385176
http://linked.open...riv/jazykVysledku
http://linked.open.../riv/klicovaSlova
  • Abies alba; A. numidica; extracellular matrix (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...odStatuVydavatele
  • NL - Nizozemsko
http://linked.open...ontrolniKodProRIV
  • [1D3E1F038882]
http://linked.open...i/riv/nazevZdroje
  • Biologia Plantarum
http://linked.open...in/vavai/riv/obor
http://linked.open...ichTvurcuVysledku
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http://linked.open...vavai/riv/projekt
http://linked.open...UplatneniVysledku
http://linked.open...v/svazekPeriodika
  • 56
http://linked.open...iv/tvurceVysledku
  • Neděla, Vilém
  • Hřib, J.
  • Vooková, B.
http://linked.open...ain/vavai/riv/wos
  • 000303062400028
issn
  • 0006-3134
number of pages
http://bibframe.org/vocab/doi
  • 10.1007/s10535-012-0062-x
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