About: Employment of laser induced fusion of living cells for the study of spatial structure of chromatin.

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About: Employment of laser induced fusion of living cells for the study of spatial structure of chromatin. Goto Sponge NotDistinct Permalink

An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	We study the transfer of the cell nucleus and individual chromosomes from one living cell to the other one during their fusion. To achieve this, the nuclei of the two fused cells are stained with different fluorescent dyes which serve as identification markers. The fusion itself is done in an inverted optical microscope by combined system that uses optical tweezers to bring two living cells into contact and optical scalpel to punctuate their membranes at the contact point. This process initiates a fusion of both cells into one hybrid cell containing two nuclei. If the fusion product is viable, these nuclei tend to mix together. The dynamics of the fusion process is then visualized by exciting the fluorescently labeled fusion product with a suitable light source. The time evolution of the mutual position of the fused cell nuclei and their final orientation is traced from a video record of the experiment. We study the transfer of the cell nucleus and individual chromosomes from one living cell to the other one during their fusion. To achieve this, the nuclei of the two fused cells are stained with different fluorescent dyes which serve as identification markers. The fusion itself is done in an inverted optical microscope by combined system that uses optical tweezers to bring two living cells into contact and optical scalpel to punctuate their membranes at the contact point. This process initiates a fusion of both cells into one hybrid cell containing two nuclei. If the fusion product is viable, these nuclei tend to mix together. The dynamics of the fusion process is then visualized by exciting the fluorescently labeled fusion product with a suitable light source. The time evolution of the mutual position of the fused cell nuclei and their final orientation is traced from a video record of the experiment. (en)
Title	Employment of laser induced fusion of living cells for the study of spatial structure of chromatin. Employment of laser induced fusion of living cells for the study of spatial structure of chromatin. (en)
skos:prefLabel	Employment of laser induced fusion of living cells for the study of spatial structure of chromatin. Employment of laser induced fusion of living cells for the study of spatial structure of chromatin. (en)
skos:notation	RIV/68081731:_____/03:12030097!RIV/2004/AV0/A12004/N
http://linked.open.../vavai/riv/strany	336 ; 340
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(IAA1065203), Z(AV0Z2065902), Z(AV0Z5004920)
http://linked.open...vai/riv/dodaniDat	2004
http://linked.open...aciTvurceVysledku	Ježek, Jan Zemánek, Pavel Jonáš, Alexandr Šerý, Mojmír Jákl, Petr
http://linked.open.../riv/druhVysledku	D - Článek ve sborníku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Ústav přístrojové techniky AV ČR, v. v. i.
http://linked.open...dnocenehoVysledku	605869
http://linked.open...ai/riv/idVysledku	RIV/68081731:_____/03:12030097
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	laser trapping; optical tweezers; laser scissors (en)
http://linked.open.../riv/klicoveSlovo	optical tweezers laser trapping laser scissors
http://linked.open...ontrolniKodProRIV	[B61C30B33523]
http://linked.open...v/mistoKonaniAkce	Krzyzowa [PL]
http://linked.open...i/riv/mistoVydani	Washington
http://linked.open...i/riv/nazevZdroje	Proceedings of the 13th Polish-Czech-Slovak Optical Conference on Wave and Quantum Aspects of Contemporary Optics (Proc. SPIE 5259).
http://linked.open...in/vavai/riv/obor	BH
http://linked.open...ichTvurcuVysledku	5 (xsd:int)
http://linked.open...cetTvurcuVysledku	9 (xsd:int)
http://linked.open...ocetUcastnikuAkce	0 (xsd:int)
http://linked.open...nichUcastnikuAkce	0 (xsd:int)
http://linked.open...vavai/riv/projekt	The use of combination of laser microbeam and cytometric techniques in the study of the structure and dynamics of human genome
http://linked.open...UplatneniVysledku	2003
http://linked.open...iv/tvurceVysledku	Ježek, Jan Jonáš, Alexandr Kozubek, Stanislav Liška, M. Lukášová, Emilie Zemánek, Pavel Jákl, Petr Šerý, Mojmír Palša, Stanislav
http://linked.open...vavai/riv/typAkce	WRD - Světová
http://linked.open.../riv/zahajeniAkce	2002-09-09 (xsd:date)
http://linked.open...n/vavai/riv/zamer	Rozvoj fyzikálních metod, speciálních technologií a přístrojových principů využívajících elektronových a světelných svazků a radiofrekvenční spektroskopie Biophysical properties of living systems and their changes under the influence of environmental factors
number of pages	5 (xsd:int)
http://purl.org/ne...btex#hasPublisher	SPIE-The International Society for Optical Engineering

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