About: Spatial structure of chromatin in hybrid cells produced by laser induced fusion studied by optical microscopy.

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About: Spatial structure of chromatin in hybrid cells produced by laser induced fusion studied by optical microscopy. Goto Sponge NotDistinct Permalink

An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	In this article we describe a combined system that uses optical tweezers to bring two living cells into contact and optical scalpel to puncuate their membranes at the contact point. This process inititates a fusion of both cells into one hybrid cell containing two nuclei. If the fusion product is viable, these nuclei tend to mix together. The spatial distribution of the nuclear material in the resulting hybrid nucleus is studied by analysis of positions of FISH (fluorescent hybridization in situ) signals of specific genetic loci in automated fluorescence microscope (high resolution cytometer). The obtained results are compared to the signals distribution of FISH in the original cells. In this article we describe a combined system that uses optical tweezers to bring two living cells into contact and optical scalpel to puncuate their membranes at the contact point. This process inititates a fusion of both cells into one hybrid cell containing two nuclei. If the fusion product is viable, these nuclei tend to mix together. The spatial distribution of the nuclear material in the resulting hybrid nucleus is studied by analysis of positions of FISH (fluorescent hybridization in situ) signals of specific genetic loci in automated fluorescence microscope (high resolution cytometer). The obtained results are compared to the signals distribution of FISH in the original cells. (en)
Title	Spatial structure of chromatin in hybrid cells produced by laser induced fusion studied by optical microscopy. Spatial structure of chromatin in hybrid cells produced by laser induced fusion studied by optical microscopy. (en)
skos:prefLabel	Spatial structure of chromatin in hybrid cells produced by laser induced fusion studied by optical microscopy. Spatial structure of chromatin in hybrid cells produced by laser induced fusion studied by optical microscopy. (en)
skos:notation	RIV/68081731:_____/02:12030014!RIV/2004/AV0/A12004/N
http://linked.open.../vavai/riv/strany	630 ; 634
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(IAA1065203), Z(AV0Z2065902)
http://linked.open...vai/riv/dodaniDat	2004
http://linked.open...aciTvurceVysledku	Ježek, Jan Zemánek, Pavel Jonáš, Alexandr Šerý, Mojmír Jákl, Petr
http://linked.open.../riv/druhVysledku	D - Článek ve sborníku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Ústav přístrojové techniky AV ČR, v. v. i.
http://linked.open...dnocenehoVysledku	664448
http://linked.open...ai/riv/idVysledku	RIV/68081731:_____/02:12030014
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	laser trapping; optical tweezers; laser scissors (en)
http://linked.open.../riv/klicoveSlovo	optical tweezers laser trapping laser scissors
http://linked.open...ontrolniKodProRIV	[89698DA2904A]
http://linked.open...v/mistoKonaniAkce	Praha [CZ]
http://linked.open...i/riv/mistoVydani	Prague
http://linked.open...i/riv/nazevZdroje	Proceedings of SPIE - The International society for optical engineering. Photonic, devices, and systems II.
http://linked.open...in/vavai/riv/obor	BH
http://linked.open...ichTvurcuVysledku	5 (xsd:int)
http://linked.open...cetTvurcuVysledku	9 (xsd:int)
http://linked.open...ocetUcastnikuAkce	0 (xsd:int)
http://linked.open...nichUcastnikuAkce	0 (xsd:int)
http://linked.open...vavai/riv/projekt	The use of combination of laser microbeam and cytometric techniques in the study of the structure and dynamics of human genome
http://linked.open...UplatneniVysledku	2002
http://linked.open...iv/tvurceVysledku	Ježek, Jan Jonáš, Alexandr Liška, M. Zemánek, Pavel Jákl, Petr Šerý, Mojmír Lukášová, E. Palša, S. Kozubek, S.
http://linked.open...vavai/riv/typAkce	WRD - Světová
http://linked.open.../riv/zahajeniAkce	2002-05-26 (xsd:date)
http://linked.open...n/vavai/riv/zamer	Rozvoj fyzikálních metod, speciálních technologií a přístrojových principů využívajících elektronových a světelných svazků a radiofrekvenční spektroskopie
number of pages	5 (xsd:int)
http://purl.org/ne...btex#hasPublisher	SPIE

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