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Description
  • This work describes an experimental method for the measurement of the profile of the laser beam focused by an immersion microscope objective. The method makes use of the two-photon excited fluorescence emitted by a 200 nm dyed polystyrene bead fixed to the cover slip. A piezo driven stage is used for scanning of the bead through the focal volume of an infrared laser beam and the excited two-photon fluorescence is detected by a photomultiplier.
  • This work describes an experimental method for the measurement of the profile of the laser beam focused by an immersion microscope objective. The method makes use of the two-photon excited fluorescence emitted by a 200 nm dyed polystyrene bead fixed to the cover slip. A piezo driven stage is used for scanning of the bead through the focal volume of an infrared laser beam and the excited two-photon fluorescence is detected by a photomultiplier. (en)
Title
  • Measurement of Intensity Profile in the Focal Region of Microscope Objective.
  • Measurement of Intensity Profile in the Focal Region of Microscope Objective. (en)
skos:prefLabel
  • Measurement of Intensity Profile in the Focal Region of Microscope Objective.
  • Measurement of Intensity Profile in the Focal Region of Microscope Objective. (en)
skos:notation
  • RIV/68081731:_____/01:12010064!RIV/2003/AV0/A12003/N
http://linked.open.../vavai/riv/strany
  • 410;414
http://linked.open...avai/riv/aktivita
http://linked.open...avai/riv/aktivity
  • Z(AV0Z2065902)
http://linked.open...vai/riv/dodaniDat
http://linked.open...aciTvurceVysledku
http://linked.open.../riv/druhVysledku
http://linked.open...iv/duvernostUdaju
http://linked.open...titaPredkladatele
http://linked.open...dnocenehoVysledku
  • 686220
http://linked.open...ai/riv/idVysledku
  • RIV/68081731:_____/01:12010064
http://linked.open...riv/jazykVysledku
http://linked.open.../riv/klicovaSlova
  • two-photon fluorescence; focused beam; intensity measurement (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...ontrolniKodProRIV
  • [7BB2C21A7731]
http://linked.open...v/mistoKonaniAkce
  • Brno [CZ]
http://linked.open...i/riv/mistoVydani
  • Brno
http://linked.open...i/riv/nazevZdroje
  • Sborník příspěvků konference Nové trendy ve fyzice.
http://linked.open...in/vavai/riv/obor
http://linked.open...ichTvurcuVysledku
http://linked.open...cetTvurcuVysledku
http://linked.open...ocetUcastnikuAkce
http://linked.open...nichUcastnikuAkce
http://linked.open...UplatneniVysledku
http://linked.open...iv/tvurceVysledku
  • Ježek, Jan
  • Jonáš, Alexandr
  • Liška, M.
  • Zemánek, Pavel
  • Jákl, Petr
  • Šerý, Mojmír
http://linked.open...vavai/riv/typAkce
http://linked.open.../riv/zahajeniAkce
http://linked.open...n/vavai/riv/zamer
number of pages
http://purl.org/ne...btex#hasPublisher
  • Vysoké učení technické v Brně
https://schema.org/isbn
  • 80-214-1992-X
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