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  • The idea of observation of biological specimens at their natural state is connected with the very beginning of electron microscopy. In the forties of the past century different preparation techniques were tested with the intention to separate a specimen with high content of water from destructive influence of vacuum by convenient means. Satisfactory results were achieved as late as in the eighties. The principle of environmental scanning electron microscopy is based on separation of high vacuum space from the specimen chamber by a differential chamber placed between the specimen chamber and high-vacuum part of the electron-optical column. The detection of secondary electrons and backscattered electrons at pressure about 1000 Pa is provided by ionisation or scintillation detector.
  • The idea of observation of biological specimens at their natural state is connected with the very beginning of electron microscopy. In the forties of the past century different preparation techniques were tested with the intention to separate a specimen with high content of water from destructive influence of vacuum by convenient means. Satisfactory results were achieved as late as in the eighties. The principle of environmental scanning electron microscopy is based on separation of high vacuum space from the specimen chamber by a differential chamber placed between the specimen chamber and high-vacuum part of the electron-optical column. The detection of secondary electrons and backscattered electrons at pressure about 1000 Pa is provided by ionisation or scintillation detector. (en)
Title
  • Electron Microscopy of Biological Specimens at Their Natural State.
  • Electron Microscopy of Biological Specimens at Their Natural State. (en)
skos:prefLabel
  • Electron Microscopy of Biological Specimens at Their Natural State.
  • Electron Microscopy of Biological Specimens at Their Natural State. (en)
skos:notation
  • RIV/68081731:_____/01:12010047!RIV/2003/AV0/A12003/N
http://linked.open.../vavai/riv/strany
  • 63;64
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  • P(GA102/01/1271), Z(AV0Z2065902)
http://linked.open...vai/riv/dodaniDat
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  • 679142
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  • RIV/68081731:_____/01:12010047
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  • electron microscopy; specimen chamber (en)
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http://linked.open...ontrolniKodProRIV
  • [4E8BBC672096]
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  • Lecce [IT]
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  • Lecce
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  • Proceedings of 5th Multinational Congress on Electron Microscopy.
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  • Autrata, Rudolf
  • Procházka, V.
  • Horký, D.
  • Ilkovics, L.
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http://linked.open.../riv/zahajeniAkce
http://linked.open...n/vavai/riv/zamer
number of pages
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  • Rinton Press
https://schema.org/isbn
  • 1-58949-003-7
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