About: Simple power supply for power load controlled isoelectric focusing

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An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

Attributes	Values
rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
rdfs:seeAlso	http://hdl.handle.net/11104/0231022
Description	The power supply for IEF based on features of the Cockcroft-Walton voltage multiplier (CW VM) is described in this work. The article describes a design of the IEF power supply, its electric characteristics, and testing by IEF analysis. A circuit diagram of the power supply included two opposite charged branches (each consisting of four voltage doublers). The designed CW VM was powered by 230 V/50 Hz alternate current and it generated up to 5 kV and 90 mW at the output. Voltage and current characteristics of the power supply were measured by known load resistances in the range from 10 k to 1 G , which is a common resistance range for IEF strip geometry. Further, the power supply was tested by a separation of a model mixture of colored pI markers using a 175 3 0.5 mm focusing bed. Automatically limited power load enabled analysis of samples without previous optimization of the focusing voltage or electric current time courses according to sample composition. Moreover, the developed power supply did not produce any intrinsic heat and was easy to set up with cheap and commonly available parts. The power supply for IEF based on features of the Cockcroft-Walton voltage multiplier (CW VM) is described in this work. The article describes a design of the IEF power supply, its electric characteristics, and testing by IEF analysis. A circuit diagram of the power supply included two opposite charged branches (each consisting of four voltage doublers). The designed CW VM was powered by 230 V/50 Hz alternate current and it generated up to 5 kV and 90 mW at the output. Voltage and current characteristics of the power supply were measured by known load resistances in the range from 10 k to 1 G , which is a common resistance range for IEF strip geometry. Further, the power supply was tested by a separation of a model mixture of colored pI markers using a 175 3 0.5 mm focusing bed. Automatically limited power load enabled analysis of samples without previous optimization of the focusing voltage or electric current time courses according to sample composition. Moreover, the developed power supply did not produce any intrinsic heat and was easy to set up with cheap and commonly available parts. (en)
Title	Simple power supply for power load controlled isoelectric focusing Simple power supply for power load controlled isoelectric focusing (en)
skos:prefLabel	Simple power supply for power load controlled isoelectric focusing Simple power supply for power load controlled isoelectric focusing (en)
skos:notation	RIV/68081715:_____/14:00425057!RIV15-MV0-68081715
http://linked.open...avai/riv/aktivita	I P
http://linked.open...avai/riv/aktivity	I, P(VG20102015023)
http://linked.open...iv/cisloPeriodika	8
http://linked.open...vai/riv/dodaniDat	2015
http://linked.open...aciTvurceVysledku	Šlais, Karel Duša, Filip
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Ústav analytické chemie AV ČR, v. v. i.
http://linked.open...dnocenehoVysledku	44894
http://linked.open...ai/riv/idVysledku	RIV/68081715:_____/14:00425057
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	isoelectric focusing; power supply; voltage multiplier (en)
http://linked.open.../riv/klicoveSlovo	isoelectric focusing voltage multiplier power supply
http://linked.open...odStatuVydavatele	DE - Spolková republika Německo
http://linked.open...ontrolniKodProRIV	[67D1CDAE4758]
http://linked.open...i/riv/nazevZdroje	Electrophoresis
http://linked.open...in/vavai/riv/obor	CB
http://linked.open...ichTvurcuVysledku	2 (xsd:int)
http://linked.open...cetTvurcuVysledku	2 (xsd:int)
http://linked.open...vavai/riv/projekt	Rapid Decision Systems for Food Safety
http://linked.open...UplatneniVysledku	2014
http://linked.open...v/svazekPeriodika	35
http://linked.open...iv/tvurceVysledku	Šlais, Karel Duša, Filip
http://linked.open...ain/vavai/riv/wos	000334126600005
issn	0173-0835
number of pages	4 (xsd:int)
http://bibframe.org/vocab/doi	10.1002/elps.201300518

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