About: Multi-step synthesis of caspase-3 sensor based on Förster resonance energy transfer

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About: Multi-step synthesis of caspase-3 sensor based on Förster resonance energy transfer Goto Sponge NotDistinct Permalink

An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Programmed cell death or apoptosis is regulated process of cell suicide. The central role in apoptosis play cysteine proteases called caspases. Caspases recognize tetra-peptide sequences Asp-Glu-Val-Asp (DEVD) on their substrates and hydrolyze peptide bonds after aspartic acid residues. Various techniques for the determination of caspase-3 are commercially available e.g. Enzyme Linked Immuno-Sorbent Assay (ELISA), Western blotting or flow cytometric analysis. The products of the cleavage can be detected by spectrophotometry, fluorimetry, chemiluminescence (CL) or ELISA. In this work, we suggested fluorescent sensor based on Förster Resonance Energy Transfer (FRET) to determine caspase-3 in cell nucleus or cytoplasm by apoptosis for very fast analysis by fluorescence microscopy without sample destroying. Programmed cell death or apoptosis is regulated process of cell suicide. The central role in apoptosis play cysteine proteases called caspases. Caspases recognize tetra-peptide sequences Asp-Glu-Val-Asp (DEVD) on their substrates and hydrolyze peptide bonds after aspartic acid residues. Various techniques for the determination of caspase-3 are commercially available e.g. Enzyme Linked Immuno-Sorbent Assay (ELISA), Western blotting or flow cytometric analysis. The products of the cleavage can be detected by spectrophotometry, fluorimetry, chemiluminescence (CL) or ELISA. In this work, we suggested fluorescent sensor based on Förster Resonance Energy Transfer (FRET) to determine caspase-3 in cell nucleus or cytoplasm by apoptosis for very fast analysis by fluorescence microscopy without sample destroying. (en)
Title	Multi-step synthesis of caspase-3 sensor based on Förster resonance energy transfer Multi-step synthesis of caspase-3 sensor based on Förster resonance energy transfer (en)
skos:prefLabel	Multi-step synthesis of caspase-3 sensor based on Förster resonance energy transfer Multi-step synthesis of caspase-3 sensor based on Förster resonance energy transfer (en)
skos:notation	RIV/68081715:_____/13:00422636!RIV14-MSM-68081715
http://linked.open...avai/riv/aktivita	I P
http://linked.open...avai/riv/aktivity	I, P(EE2.3.20.0182), P(GAP206/11/2377), P(GAP301/11/2055), P(GBP206/12/G014)
http://linked.open...vai/riv/dodaniDat	2014
http://linked.open...aciTvurceVysledku	Lišková, Marcela Foret, František Klepárník, Karel
http://linked.open.../riv/druhVysledku	D - Článek ve sborníku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Ústav analytické chemie AV ČR, v. v. i.
http://linked.open...dnocenehoVysledku	90189
http://linked.open...ai/riv/idVysledku	RIV/68081715:_____/13:00422636
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	caspase-3; Förster resonance energy transfer; apoptotic cells (en)
http://linked.open.../riv/klicoveSlovo	Förster resonance energy transfer apoptotic cells caspase-3
http://linked.open...ontrolniKodProRIV	[16A831EFC419]
http://linked.open...v/mistoKonaniAkce	Brno
http://linked.open...i/riv/mistoVydani	Praha
http://linked.open...i/riv/nazevZdroje	Chemické Listy. Roč. 107, Issue s3.
http://linked.open...in/vavai/riv/obor	CB
http://linked.open...ichTvurcuVysledku	3 (xsd:int)
http://linked.open...cetTvurcuVysledku	4 (xsd:int)
http://linked.open...vavai/riv/projekt	Single-cell analysis by mass spectrometry New methods of analysis of proteins and their glycosylation in cancer - combination of electrochemistry, microfludic biosensors and mass spectrometry. Development of Multidisciplinary Research and Educational Centrum for Bioanalytical Technologies Center for advanced bioanalytical technologies
http://linked.open...UplatneniVysledku	2013
http://linked.open...iv/tvurceVysledku	Foret, František Klepárník, Karel Lišková, Marcela Pazdera, P.
http://linked.open...vavai/riv/typAkce	WRD - Světová
http://linked.open...ain/vavai/riv/wos	000328730800013
http://linked.open.../riv/zahajeniAkce	2013-11-12 (xsd:date)
issn	1213-7103
number of pages	3 (xsd:int)
http://purl.org/ne...btex#hasPublisher	Česká společnost chemická

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