About: Senzitive method of Caspase-3 detection in single stem cell

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An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Caspases are involved in physiological process (e.g. cellular differentiation) but also can cause serious disorders. It is getting necessary to develop sensitive, miniaturized and fast methods amenable to analyze small amounts of samples. Luciferin/luciferase chemiluminescence reaction is one of the methods of caspase-3 detection. We have developed a miniaturized device enabling detection of caspase-3 and quantitation just in femtogram level (10–15 g) in single apoptotic human stem cells (neural crest derived). The technology is based on the specific cleavage of modified luciferin by caspase-3, emissions of photons and their detection by photomultiplier tube working in the photon counting regime. Caspases are involved in physiological process (e.g. cellular differentiation) but also can cause serious disorders. It is getting necessary to develop sensitive, miniaturized and fast methods amenable to analyze small amounts of samples. Luciferin/luciferase chemiluminescence reaction is one of the methods of caspase-3 detection. We have developed a miniaturized device enabling detection of caspase-3 and quantitation just in femtogram level (10–15 g) in single apoptotic human stem cells (neural crest derived). The technology is based on the specific cleavage of modified luciferin by caspase-3, emissions of photons and their detection by photomultiplier tube working in the photon counting regime. (en)
Title	Senzitive method of Caspase-3 detection in single stem cell Senzitive method of Caspase-3 detection in single stem cell (en)
skos:prefLabel	Senzitive method of Caspase-3 detection in single stem cell Senzitive method of Caspase-3 detection in single stem cell (en)
skos:notation	RIV/68081715:_____/13:00420849!RIV14-MSM-68081715
http://linked.open...avai/riv/aktivita	I P
http://linked.open...avai/riv/aktivity	I, P(EE2.3.20.0182), P(GAP206/11/2377)
http://linked.open...vai/riv/dodaniDat	2014
http://linked.open...aciTvurceVysledku	Klepárník, Karel Adamová, Eva
http://linked.open.../riv/druhVysledku	D - Článek ve sborníku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Ústav analytické chemie AV ČR, v. v. i.
http://linked.open...dnocenehoVysledku	104701
http://linked.open...ai/riv/idVysledku	RIV/68081715:_____/13:00420849
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	caspase-3; single stem cell; miniaturization (en)
http://linked.open.../riv/klicoveSlovo	single stem cell miniaturization caspase-3
http://linked.open...ontrolniKodProRIV	[85C6F701EFA0]
http://linked.open...v/mistoKonaniAkce	Brno
http://linked.open...i/riv/mistoVydani	Praha
http://linked.open...i/riv/nazevZdroje	Chemické Listy. Roč. 107, Issue s3.
http://linked.open...in/vavai/riv/obor	CB
http://linked.open...ichTvurcuVysledku	2 (xsd:int)
http://linked.open...cetTvurcuVysledku	3 (xsd:int)
http://linked.open...vavai/riv/projekt	Single-cell analysis by mass spectrometry Development of Multidisciplinary Research and Educational Centrum for Bioanalytical Technologies
http://linked.open...UplatneniVysledku	2013
http://linked.open...iv/tvurceVysledku	Klepárník, Karel Matalová, Eva Adamová, Eva
http://linked.open...vavai/riv/typAkce	WRD - Světová
http://linked.open...ain/vavai/riv/wos	000328730800002
http://linked.open.../riv/zahajeniAkce	2013-11-12 (xsd:date)
issn	1213-7103
number of pages	2 (xsd:int)
http://purl.org/ne...btex#hasPublisher	Česká společnost chemická

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