About: An efficient proteomic approach to analyze agriculture crop biomass

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An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	While a plant cell wall is formed by a complex of various components, including polysaccharides and structural proteins, its composition and representation may vary during cell growth. Currently, plant research targets the proteins participating in wall loosening. Multiple classes of enzymes, including various hemicellulases and cellulases, are required for plant material degradation to achieve the maximum decomposition. Identifying the set of proteins involved in the breakdown of cell-wall polymers is important to understand plant material conversion into suitable products. The objective of this study was to describe a method which can be used to carry out proteomics analysis of complex plant samples and identify enzymes degrading biomass. For this purpose we used proteomic techniques including gel electrophoresis, high pressure liquid chromatography combinated with mass spectrometry followed by data evaluation using databases searching. Results show that more than 50 % of these activities correspond to enzymes with proteolytic function. This study was focused primarily on enzymes able to breakdown the lignocellulosic and hemicellulosic parts that are very important for the material conversion into required products of degradation. While a plant cell wall is formed by a complex of various components, including polysaccharides and structural proteins, its composition and representation may vary during cell growth. Currently, plant research targets the proteins participating in wall loosening. Multiple classes of enzymes, including various hemicellulases and cellulases, are required for plant material degradation to achieve the maximum decomposition. Identifying the set of proteins involved in the breakdown of cell-wall polymers is important to understand plant material conversion into suitable products. The objective of this study was to describe a method which can be used to carry out proteomics analysis of complex plant samples and identify enzymes degrading biomass. For this purpose we used proteomic techniques including gel electrophoresis, high pressure liquid chromatography combinated with mass spectrometry followed by data evaluation using databases searching. Results show that more than 50 % of these activities correspond to enzymes with proteolytic function. This study was focused primarily on enzymes able to breakdown the lignocellulosic and hemicellulosic parts that are very important for the material conversion into required products of degradation. (en)
Title	An efficient proteomic approach to analyze agriculture crop biomass An efficient proteomic approach to analyze agriculture crop biomass (en)
skos:prefLabel	An efficient proteomic approach to analyze agriculture crop biomass An efficient proteomic approach to analyze agriculture crop biomass (en)
skos:notation	RIV/68081715:_____/13:00395793!RIV14-MSM-68081715
http://linked.open...avai/riv/aktivita	I P
http://linked.open...avai/riv/aktivity	I, P(1M0570)
http://linked.open...iv/cisloPeriodika	5
http://linked.open...vai/riv/dodaniDat	2014
http://linked.open...aciTvurceVysledku	Flodrová, Dana Bobálová, Janette
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Ústav analytické chemie AV ČR, v. v. i.
http://linked.open...dnocenehoVysledku	60330
http://linked.open...ai/riv/idVysledku	RIV/68081715:_____/13:00395793
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	MALDI; biomass; proteomics; identification; hemicellulases (en)
http://linked.open.../riv/klicoveSlovo	hemicellulases biomass identification proteomics MALDI
http://linked.open...odStatuVydavatele	DK - Dánské království
http://linked.open...ontrolniKodProRIV	[5E83155B0F2D]
http://linked.open...i/riv/nazevZdroje	Protein Journal
http://linked.open...in/vavai/riv/obor	CB
http://linked.open...ichTvurcuVysledku	2 (xsd:int)
http://linked.open...cetTvurcuVysledku	2 (xsd:int)
http://linked.open...vavai/riv/projekt	Research Centre for Study of Extract Compounds of Barley and Hop
http://linked.open...UplatneniVysledku	2013
http://linked.open...v/svazekPeriodika	32
http://linked.open...iv/tvurceVysledku	Bobálová, Janette Flodrová, Dana
http://linked.open...ain/vavai/riv/wos	000321625500005
issn	1572-3887
number of pages	8 (xsd:int)
http://bibframe.org/vocab/doi	10.1007/s10930-013-9495-0

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