About: Oriented immobilization of PNgase F on a porous polymer monolith for rapid N-glycan release

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About: Oriented immobilization of PNgase F on a porous polymer monolith for rapid N-glycan release Goto Sponge NotDistinct Permalink

An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
rdfs:seeAlso	http://hdl.handle.net/11104/0215253
Description	We demonstrate a simple and rapid method for the oriented immobilization of peptide-N4-(Nacetyl- glucosaminyl) asparagine amidase F (PNGase F) on a porous polymer monolith. The oriented immobilization is based on the affinity of glutathione-S-transferase (GST) tagged PNGase F towards glutathione modified monolith prepared in the capillary format. This approach allows the oriented and easily replaceable immobilization of PNGase F for rapid and efficient release of N-linked glycans. The reactor was tested by deglycosylation of several native glycoproteins such as ribonuclease B, fetuin or human immunoglobulin G. The proteins were effectively deglycosylated in several minutes and the released N-linked glycans were analyzed using off-line MALDI/MS or CE/LIF. We demonstrate a simple and rapid method for the oriented immobilization of peptide-N4-(Nacetyl- glucosaminyl) asparagine amidase F (PNGase F) on a porous polymer monolith. The oriented immobilization is based on the affinity of glutathione-S-transferase (GST) tagged PNGase F towards glutathione modified monolith prepared in the capillary format. This approach allows the oriented and easily replaceable immobilization of PNGase F for rapid and efficient release of N-linked glycans. The reactor was tested by deglycosylation of several native glycoproteins such as ribonuclease B, fetuin or human immunoglobulin G. The proteins were effectively deglycosylated in several minutes and the released N-linked glycans were analyzed using off-line MALDI/MS or CE/LIF. (en)
Title	Oriented immobilization of PNgase F on a porous polymer monolith for rapid N-glycan release Oriented immobilization of PNgase F on a porous polymer monolith for rapid N-glycan release (en)
skos:prefLabel	Oriented immobilization of PNgase F on a porous polymer monolith for rapid N-glycan release Oriented immobilization of PNgase F on a porous polymer monolith for rapid N-glycan release (en)
skos:notation	RIV/68081715:_____/12:00386014!RIV13-AV0-68081715
http://linked.open...avai/riv/aktivita	I
http://linked.open...avai/riv/aktivity	I
http://linked.open...vai/riv/dodaniDat	2013
http://linked.open...aciTvurceVysledku	Foret, František Křenková, Jana
http://linked.open.../riv/druhVysledku	D - Článek ve sborníku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Ústav analytické chemie AV ČR, v. v. i.
http://linked.open...dnocenehoVysledku	157114
http://linked.open...ai/riv/idVysledku	RIV/68081715:_____/12:00386014
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	PNGase F; oriented immobilization; monolith (en)
http://linked.open.../riv/klicoveSlovo	monolith PNGase F oriented immobilization
http://linked.open...ontrolniKodProRIV	[D879290E2330]
http://linked.open...v/mistoKonaniAkce	Brno
http://linked.open...i/riv/mistoVydani	Brno
http://linked.open...i/riv/nazevZdroje	CECE 2012. 9th International Interdisciplinary Meeting on Bioanalysis
http://linked.open...in/vavai/riv/obor	CB
http://linked.open...ichTvurcuVysledku	2 (xsd:int)
http://linked.open...cetTvurcuVysledku	5 (xsd:int)
http://linked.open...UplatneniVysledku	2012
http://linked.open...iv/tvurceVysledku	Foret, František Křenková, Jana Guttman, A. Keresztessy, Z. Szekrényes, A.
http://linked.open...vavai/riv/typAkce	WRD - Světová
http://linked.open.../riv/zahajeniAkce	2012-11-01 (xsd:date)
number of pages	3 (xsd:int)
http://purl.org/ne...btex#hasPublisher	Ústav analytické chemie AV ČR, v. v. i
https://schema.org/isbn	978-80-904959-1-3

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