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  • The surfaces of biofilm-positive microorganisms are usually covered with biofilm-specific extracellular polysaccharide substances that play a key role in a biofilm formation and function [1,2] The presence of this substance on the surface can affect the physicochemical properties of the bacterial cell, including the cell-surface hydrophobicity and surface charge The differences in the surface charges lead to the different isoelectric points and the different electromigration characteristics of biofilm-positive and biofilm-negative cells For this reason, the use of the capillary electrophoresis, especially capillary isoelectric focusing (CIEF) and capillary zone electrophoresis (CZE) [3,4], appears to be the appropriate method for the detection of biofilm positivity in a particular strain, as well as for the separation of biofilm-positive and biofilm-negative strains.
  • The surfaces of biofilm-positive microorganisms are usually covered with biofilm-specific extracellular polysaccharide substances that play a key role in a biofilm formation and function [1,2] The presence of this substance on the surface can affect the physicochemical properties of the bacterial cell, including the cell-surface hydrophobicity and surface charge The differences in the surface charges lead to the different isoelectric points and the different electromigration characteristics of biofilm-positive and biofilm-negative cells For this reason, the use of the capillary electrophoresis, especially capillary isoelectric focusing (CIEF) and capillary zone electrophoresis (CZE) [3,4], appears to be the appropriate method for the detection of biofilm positivity in a particular strain, as well as for the separation of biofilm-positive and biofilm-negative strains. (en)
Title
  • Extracellular Polysaccharides in Microbial Biofilm and Their Influence on the Electrophoretic Properties of Microbial Cells
  • Extracellular Polysaccharides in Microbial Biofilm and Their Influence on the Electrophoretic Properties of Microbial Cells (en)
skos:prefLabel
  • Extracellular Polysaccharides in Microbial Biofilm and Their Influence on the Electrophoretic Properties of Microbial Cells
  • Extracellular Polysaccharides in Microbial Biofilm and Their Influence on the Electrophoretic Properties of Microbial Cells (en)
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  • RIV/68081715:_____/11:00437343!RIV15-AV0-68081715
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  • RIV/68081715:_____/11:00437343
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  • capillary zone electrophoresis; laser induced flurescence; high performance electrophoresis (en)
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  • Capillary Electrophoresis of Carbohydrates: From Monosaccharides to Complex Plysaccharides
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  • Růžička, F.
  • Horká, Marie
  • Holá, V.
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number of pages
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  • 10.1007/978-1-60761-875-1_5
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  • Humana Press Inc
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  • 978-1-60761-874-4
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