About: Two-dimensional gel isoelectric focusing

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	The unique properties of IEF, focusing and resolution power, were enhanced by using the same technique twice in two directions of the same gel. Two forms of beta-lactoglobulin (pI values 5.14 and 5.31) nonseparated in the first IEF were successfully separated in the second dimension at relatively low voltage (330 V) with the resolution power comparable to the high-resolusion gels requiring the high voltage during the run and long separation time. Glucose oxidase loaded as diluted solution into ten positions across the gel was finally focused into a single band during 2-D gel IEF. Since the first and second IEF are carried out on the same gel, no losses and contamination of analyte occur. The suggested method can be used for separation/fractionation of complex biological mixtures followed by e.g. mass spectrometry analysis. The unique properties of IEF, focusing and resolution power, were enhanced by using the same technique twice in two directions of the same gel. Two forms of beta-lactoglobulin (pI values 5.14 and 5.31) nonseparated in the first IEF were successfully separated in the second dimension at relatively low voltage (330 V) with the resolution power comparable to the high-resolusion gels requiring the high voltage during the run and long separation time. Glucose oxidase loaded as diluted solution into ten positions across the gel was finally focused into a single band during 2-D gel IEF. Since the first and second IEF are carried out on the same gel, no losses and contamination of analyte occur. The suggested method can be used for separation/fractionation of complex biological mixtures followed by e.g. mass spectrometry analysis. (en) Jedinečné vlastnosti IEF, fokusace a rozlišení, byly umocněny za použití této techniky ve dvou kolmých směrech dvakrát na stejném gelu. Dvě formy beta-lactoglobulinu (pI hodnoty 5.14 a 5.31) nerozdělené v první IEF byly úspěšně rozseparovány v druhém rozměru za relativně nízkého napětí (330 V) s rozlišením srovnatelným s vysokorozlišujícími gely vyžadujícími během separace vysoké napětí a dlouhý čas analýzy. Glucose oxidáza, která byla dávkována jako zředěný roztok do deseti různých míst gelu, byla nakonec během 2-D IEF zafokusována do jediné zóny. Protože první a druhý rozměr je prováděn na stejném gelu, nedochází ke ztrátám nebo kontaminaci analytu. Navržená metoda může být využita pro separaci/frakcionaci komplexních biologických směsí s následnou analýzou, např. hmotovou spektrometrií. (cs)
Title	Two-dimensional gel isoelectric focusing Dvourozměrná gelová izoelektrická fokusace (cs) Two-dimensional gel isoelectric focusing (en)
skos:prefLabel	Two-dimensional gel isoelectric focusing Dvourozměrná gelová izoelektrická fokusace (cs) Two-dimensional gel isoelectric focusing (en)
skos:notation	RIV/68081715:_____/05:00021374!RIV06-AV0-68081715
http://linked.open.../vavai/riv/strany	3586;3591
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(IAA4031302), P(IBS4031201), Z(AV0Z40310501)
http://linked.open...iv/cisloPeriodika	18
http://linked.open...vai/riv/dodaniDat	2006
http://linked.open...aciTvurceVysledku	Šťastná, Miroslava Šlais, Karel
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Ústav analytické chemie AV ČR, v. v. i.
http://linked.open...dnocenehoVysledku	547533
http://linked.open...ai/riv/idVysledku	RIV/68081715:_____/05:00021374
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	gel isoelectric focusing; pI markers; proteins (en)
http://linked.open.../riv/klicoveSlovo	gel isoelectric focusing proteins pI markers
http://linked.open...odStatuVydavatele	DE - Spolková republika Německo
http://linked.open...ontrolniKodProRIV	[305A4E1A2C0E]
http://linked.open...i/riv/nazevZdroje	Electrophoresis
http://linked.open...in/vavai/riv/obor	CB
http://linked.open...ichTvurcuVysledku	2 (xsd:int)
http://linked.open...cetTvurcuVysledku	2 (xsd:int)
http://linked.open...vavai/riv/projekt	Apparatus for automation of electrochromatographic and electrophoretic analysis Using of the fast electrophoretic separation with the high sensitive fluorometric detection for the identification of micro-organisms
http://linked.open...UplatneniVysledku	2005
http://linked.open...v/svazekPeriodika	26
http://linked.open...iv/tvurceVysledku	Šťastná, Miroslava Šlais, Karel
http://linked.open...n/vavai/riv/zamer	Moderní analytické techniky pro bioanalýzu, ekologii a nanotechnologie
issn	0173-0835
number of pages	6 (xsd:int)

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