About: Application of high-resolution capillary array electrophoresis with automated fraction collection for GeneCalling analysis of the yeast genomic DNA.

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About: Application of high-resolution capillary array electrophoresis with automated fraction collection for GeneCalling analysis of the yeast genomic DNA. Goto Sponge NotDistinct Permalink

An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Capillary array instrument was applied to transcript profiling of the yeast genomic DNA using GeneCaliing'' chemistry. The instrument integrated a 12-capillary array for DNA separation with a replaceable sieving matrix, laser-induced fluorescence detection and an automated microfraction collector. The DNA fractions, exiting the separation capillaries, were continuously deposited in a 1536-well collection plate made of agarose gel. DNA fragments recovered from selected fractions were cloned and then sequenced. Over 80% of theoretically predicted fragments could be recovered in the collected fractions, cloned and sequenced with an average redundancy of threefold. Excellent correlation of the experimentally obtained sequences with the theoretically predicted gene fragments demonstrated the suitability of capillary array electrophoresisfor micropreparative recovery of DNA fragments. This approach, useful especially for rapid DNA expression profiling of unknown genes for nonsequenced organisms, demonstrate Capillary array instrument was applied to transcript profiling of the yeast genomic DNA using GeneCaliing'' chemistry. The instrument integrated a 12-capillary array for DNA separation with a replaceable sieving matrix, laser-induced fluorescence detection and an automated microfraction collector. The DNA fractions, exiting the separation capillaries, were continuously deposited in a 1536-well collection plate made of agarose gel. DNA fragments recovered from selected fractions were cloned and then sequenced. Over 80% of theoretically predicted fragments could be recovered in the collected fractions, cloned and sequenced with an average redundancy of threefold. Excellent correlation of the experimentally obtained sequences with the theoretically predicted gene fragments demonstrated the suitability of capillary array electrophoresisfor micropreparative recovery of DNA fragments. This approach, useful especially for rapid DNA expression profiling of unknown genes for nonsequenced organisms, demonstrate (en)
Title	Application of high-resolution capillary array electrophoresis with automated fraction collection for GeneCalling analysis of the yeast genomic DNA. Application of high-resolution capillary array electrophoresis with automated fraction collection for GeneCalling analysis of the yeast genomic DNA. (en)
skos:prefLabel	Application of high-resolution capillary array electrophoresis with automated fraction collection for GeneCalling analysis of the yeast genomic DNA. Application of high-resolution capillary array electrophoresis with automated fraction collection for GeneCalling analysis of the yeast genomic DNA. (en)
skos:notation	RIV/68081715:_____/03:25033049!RIV/2004/GA0/A25004/N
http://linked.open.../vavai/riv/strany	639;647
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(GA203/00/0772), P(GA303/00/0928), Z(AV0Z4031919)
http://linked.open...iv/cisloPeriodika	4
http://linked.open...vai/riv/dodaniDat	2004
http://linked.open...aciTvurceVysledku	Foret, František Klepárník, Karel
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Ústav analytické chemie AV ČR, v. v. i.
http://linked.open...dnocenehoVysledku	598871
http://linked.open...ai/riv/idVysledku	RIV/68081715:_____/03:25033049
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	capillary array; fraction collection; gene expression profiling (en)
http://linked.open.../riv/klicoveSlovo	capillary array gene expression profiling fraction collection
http://linked.open...odStatuVydavatele	DE - Spolková republika Německo
http://linked.open...ontrolniKodProRIV	[768C6299FEC1]
http://linked.open...i/riv/nazevZdroje	Electrophoresis
http://linked.open...in/vavai/riv/obor	CB
http://linked.open...ichTvurcuVysledku	2 (xsd:int)
http://linked.open...cetTvurcuVysledku	8 (xsd:int)
http://linked.open...ocetUcastnikuAkce	0 (xsd:int)
http://linked.open...nichUcastnikuAkce	0 (xsd:int)
http://linked.open...vavai/riv/projekt	Cystinuria- biochemical and molecular biology study Development of ultrasensitive analytical methods for molecular identification and diagnostics of serious diseases
http://linked.open...UplatneniVysledku	2003
http://linked.open...v/svazekPeriodika	24
http://linked.open...iv/tvurceVysledku	Foret, František Klepárník, Karel Minarik, M. Berka, J. Karger, B. L. Ruiz-Martinez, M. C. Sosic, Z. Hammond, R.
http://linked.open...n/vavai/riv/zamer	Vyvinutí nových metod analytické chemie pro potřeby společenské praxe
issn	0173-0835
number of pages	9 (xsd:int)

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