About: Dynamics of gel isoelectric focusing with ampholytic dyes monitored by camera in real-time.

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About: Dynamics of gel isoelectric focusing with ampholytic dyes monitored by camera in real-time. Goto Sponge NotDistinct Permalink

An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	The dynamics of gel isoelectric focusing were studied by using amphoteric low-molecular-mass colored substances (isoelectric point markers).The polyacrylamide gel in slab format was in direct contact with the electrodes. In addition to isoelectric focusing with pH gradient composed of synthetic carrier ampholytes, pH gradients created by simple buffers were applied. The progress of the elerctrofocusing process was monitored by CCD camera and video recording. The gradient profile and dynamics were approximated from the positions of isoelectric point markers. The obtained animated records enabled the observation of the entire real focusing run within fractions of a minute, which is useful both for the understanding and optimization of the focusing. The dynamics of gel isoelectric focusing were studied by using amphoteric low-molecular-mass colored substances (isoelectric point markers).The polyacrylamide gel in slab format was in direct contact with the electrodes. In addition to isoelectric focusing with pH gradient composed of synthetic carrier ampholytes, pH gradients created by simple buffers were applied. The progress of the elerctrofocusing process was monitored by CCD camera and video recording. The gradient profile and dynamics were approximated from the positions of isoelectric point markers. The obtained animated records enabled the observation of the entire real focusing run within fractions of a minute, which is useful both for the understanding and optimization of the focusing. (en)
Title	Dynamics of gel isoelectric focusing with ampholytic dyes monitored by camera in real-time. Dynamics of gel isoelectric focusing with ampholytic dyes monitored by camera in real-time. (en)
skos:prefLabel	Dynamics of gel isoelectric focusing with ampholytic dyes monitored by camera in real-time. Dynamics of gel isoelectric focusing with ampholytic dyes monitored by camera in real-time. (en)
skos:notation	RIV/68081715:_____/03:25033038!RIV/2004/GA0/A25004/N
http://linked.open.../vavai/riv/strany	193;203
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(GA203/02/1447), P(IBS4031201), Z(AV0Z4031919)
http://linked.open...iv/cisloPeriodika	2
http://linked.open...vai/riv/dodaniDat	2004
http://linked.open...aciTvurceVysledku	Šťastná, Miroslava Šlais, Karel
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Ústav analytické chemie AV ČR, v. v. i.
http://linked.open...dnocenehoVysledku	604734
http://linked.open...ai/riv/idVysledku	RIV/68081715:_____/03:25033038
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Dynamics; gel isoelectric focusing; pI markers (en)
http://linked.open.../riv/klicoveSlovo	Dynamics gel isoelectric focusing pI markers
http://linked.open...odStatuVydavatele	NL - Nizozemsko
http://linked.open...ontrolniKodProRIV	[D298154CBD11]
http://linked.open...i/riv/nazevZdroje	Journal of Chromatography A
http://linked.open...in/vavai/riv/obor	CB
http://linked.open...ichTvurcuVysledku	2 (xsd:int)
http://linked.open...cetTvurcuVysledku	2 (xsd:int)
http://linked.open...ocetUcastnikuAkce	0 (xsd:int)
http://linked.open...nichUcastnikuAkce	0 (xsd:int)
http://linked.open...vavai/riv/projekt	Capillary electrochromatography in linear and planar microstructures using polymer beds Apparatus for automation of electrochromatographic and electrophoretic analysis
http://linked.open...UplatneniVysledku	2003
http://linked.open...v/svazekPeriodika	1008
http://linked.open...iv/tvurceVysledku	Šťastná, Miroslava Šlais, Karel
http://linked.open...n/vavai/riv/zamer	Vyvinutí nových metod analytické chemie pro potřeby společenské praxe
issn	0021-9673
number of pages	11 (xsd:int)

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