About: Optimization of experimental conditions for characterization of barley starch granules by gravitational field-flow fractionation.

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Separation of small and large barley starch granules by gravitational field-flow fractionation (GFFF) was investigated from the point of view of sample pre-treatment, amount of injected sample, and elution conditions. The sample pre-treatment study resulted in the conclusion that it is reasonable to soak the starch granules for at least 24 hours prior to separation. The experiments with different amounts of injected sample show that it is possible to increase twice as well as decrease twice the sample amount usually used without any change in retention ratios. The implementation of flow-rate gradients for elution of the starch granules reduced total separation time. However, the applied flow-rate gradients did not improve the resolution of the peaks A and B compared with the generally used constant flow-rate. Thus, for barley starch granules, the constant flow-rates within the range from 0.8 ml/min to 1.0 ml/min seem to provide the best compromise of total separation time, peak resolution and instrume Separation of small and large barley starch granules by gravitational field-flow fractionation (GFFF) was investigated from the point of view of sample pre-treatment, amount of injected sample, and elution conditions. The sample pre-treatment study resulted in the conclusion that it is reasonable to soak the starch granules for at least 24 hours prior to separation. The experiments with different amounts of injected sample show that it is possible to increase twice as well as decrease twice the sample amount usually used without any change in retention ratios. The implementation of flow-rate gradients for elution of the starch granules reduced total separation time. However, the applied flow-rate gradients did not improve the resolution of the peaks A and B compared with the generally used constant flow-rate. Thus, for barley starch granules, the constant flow-rates within the range from 0.8 ml/min to 1.0 ml/min seem to provide the best compromise of total separation time, peak resolution and instrume (en)
Title	Optimization of experimental conditions for characterization of barley starch granules by gravitational field-flow fractionation. Optimization of experimental conditions for characterization of barley starch granules by gravitational field-flow fractionation. (en)
skos:prefLabel	Optimization of experimental conditions for characterization of barley starch granules by gravitational field-flow fractionation. Optimization of experimental conditions for characterization of barley starch granules by gravitational field-flow fractionation. (en)
skos:notation	RIV/68081715:_____/01:25010060!RIV/2003/AV0/A25003/N
http://linked.open.../vavai/riv/strany	P3
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(IAA4031805), Z(AV0Z4031919)
http://linked.open...vai/riv/dodaniDat	2003
http://linked.open...aciTvurceVysledku	Chmelík, Josef Plocková, Jana Janoušková, Jana Budinská, Marcela Krumlová, Andrea
http://linked.open.../riv/druhVysledku	D - Článek ve sborníku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Ústav analytické chemie AV ČR, v. v. i.
http://linked.open...dnocenehoVysledku	690327
http://linked.open...ai/riv/idVysledku	RIV/68081715:_____/01:25010060
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	starch; field-flow fractionation (en)
http://linked.open.../riv/klicoveSlovo	starch field-flow fractionation
http://linked.open...ontrolniKodProRIV	[77CE93449190]
http://linked.open...v/mistoKonaniAkce	Colorado [US]
http://linked.open...i/riv/mistoVydani	Colorado
http://linked.open...i/riv/nazevZdroje	FFF-2001. Ninth International Symposium on Field-Flow Fractionation.
http://linked.open...in/vavai/riv/obor	CB
http://linked.open...ichTvurcuVysledku	5 (xsd:int)
http://linked.open...cetTvurcuVysledku	5 (xsd:int)
http://linked.open...ocetUcastnikuAkce	0 (xsd:int)
http://linked.open...nichUcastnikuAkce	0 (xsd:int)
http://linked.open...vavai/riv/projekt	Investigation of elution modes in Gravitational field-flov fractionation and application to separation of particles and cells.
http://linked.open...UplatneniVysledku	2001
http://linked.open...iv/tvurceVysledku	Janoušková, Jana Chmelík, Josef Plocková, Jana Krumlová, Andrea Budinská, Marcela
http://linked.open...vavai/riv/typAkce	WRD - Světová
http://linked.open.../riv/zahajeniAkce	2001-06-26 (xsd:date)
http://linked.open...n/vavai/riv/zamer	Vyvinutí nových metod analytické chemie pro potřeby společenské praxe
number of pages	1 (xsd:int)
http://purl.org/ne...btex#hasPublisher	Department of Chemistry and Geochemistry, Colorado School of Mines

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