About: Dynamic modification of proteins for fluorometric detection in CZE.

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An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	The separation techniques employing fluorescence detection are sensitive and selective so they have been often applied for the trace analysis of biological samples. The commonly used derivatization of proteins can lower the detection limits; however, they can change the acido-basic properties and mobilities when compared to the native species. Recently, we have used the colored tenside as a buffer additive for the photometric detection of proteins in the UV region. The selectivity, efficiency and resolution of CZE separation using this dye were found to be similar to the CZE with SDS as the additive. In this study, the ampiphilic fluorescent compound is suggested as a buffer additive in CZE for dynamic modification of the sample of several proteins. Using the deuterium lamp for the excitation in the UV region for the on column fluorometric detection, the amol minimum detectable amounts of the proteins sampled on the CZE capillary were achieved. The separation techniques employing fluorescence detection are sensitive and selective so they have been often applied for the trace analysis of biological samples. The commonly used derivatization of proteins can lower the detection limits; however, they can change the acido-basic properties and mobilities when compared to the native species. Recently, we have used the colored tenside as a buffer additive for the photometric detection of proteins in the UV region. The selectivity, efficiency and resolution of CZE separation using this dye were found to be similar to the CZE with SDS as the additive. In this study, the ampiphilic fluorescent compound is suggested as a buffer additive in CZE for dynamic modification of the sample of several proteins. Using the deuterium lamp for the excitation in the UV region for the on column fluorometric detection, the amol minimum detectable amounts of the proteins sampled on the CZE capillary were achieved. (en)
Title	Dynamic modification of proteins for fluorometric detection in CZE. Dynamic modification of proteins for fluorometric detection in CZE. (en)
skos:prefLabel	Dynamic modification of proteins for fluorometric detection in CZE. Dynamic modification of proteins for fluorometric detection in CZE. (en)
skos:notation	RIV/68081715:_____/01:25010050!RIV/2003/AV0/A25003/N
http://linked.open.../vavai/riv/strany	91
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(IAA4031901), Z(AV0Z4031919)
http://linked.open...vai/riv/dodaniDat	2003
http://linked.open...aciTvurceVysledku	Šlais, Karel Horká, Marie
http://linked.open.../riv/druhVysledku	D - Článek ve sborníku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Ústav analytické chemie AV ČR, v. v. i.
http://linked.open...dnocenehoVysledku	678360
http://linked.open...ai/riv/idVysledku	RIV/68081715:_____/01:25010050
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	capillary zone electrophoresis; fluorometric detection; dynamic modification (en)
http://linked.open.../riv/klicoveSlovo	capillary zone electrophoresis fluorometric detection dynamic modification
http://linked.open...ontrolniKodProRIV	[50911CC92743]
http://linked.open...v/mistoKonaniAkce	Praha [CZ]
http://linked.open...i/riv/mistoVydani	Praha
http://linked.open...i/riv/nazevZdroje	2nd International Symposium Separations in the BioSciences. SBS 2001.
http://linked.open...in/vavai/riv/obor	CB
http://linked.open...ichTvurcuVysledku	2 (xsd:int)
http://linked.open...cetTvurcuVysledku	2 (xsd:int)
http://linked.open...ocetUcastnikuAkce	0 (xsd:int)
http://linked.open...nichUcastnikuAkce	0 (xsd:int)
http://linked.open...vavai/riv/projekt	Capillary isotachoforesis with combined continuous conductivity and pH gradients
http://linked.open...UplatneniVysledku	2001
http://linked.open...iv/tvurceVysledku	Šlais, Karel Horká, Marie
http://linked.open...vavai/riv/typAkce	EUR - Evropská
http://linked.open.../riv/zahajeniAkce	2001-09-17 (xsd:date)
http://linked.open...n/vavai/riv/zamer	Vyvinutí nových metod analytické chemie pro potřeby společenské praxe
number of pages	1 (xsd:int)
http://purl.org/ne...btex#hasPublisher	Neuveden
https://schema.org/isbn	80-7080-437-8

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