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Description
  • This paper describes the design and application of instrumentation for automated high-throughput infusion ESI-mass spectrometry, The approach, based on a subatmospheric ESI interface, allows sample introduction from a commercially available microtiter plate without the need for a separate fluid delivery system. The microtiter plate was placed vertically on a three-dimensional translation stage in front of the sampling ESI interface. A single, 7-cm, 20-mum-i fused-silica capillary (total volume, 70 nL), with a tapered tip, served as a combination of sample delivery and spraying capillary. The tapered tip of the capillary was enclosed in a subatmospheric chamber attached in front of the orifice of the mass spectrometer. The sample aspiration rate (flow rate) was regulated by computer-controlled pneumatic valves, which allowed fast switching of the pressure in the subatmospheric ESI chamber. A flow-through wash device was positioned between the microtiter plate and the ESI interface. This design allowed
  • This paper describes the design and application of instrumentation for automated high-throughput infusion ESI-mass spectrometry, The approach, based on a subatmospheric ESI interface, allows sample introduction from a commercially available microtiter plate without the need for a separate fluid delivery system. The microtiter plate was placed vertically on a three-dimensional translation stage in front of the sampling ESI interface. A single, 7-cm, 20-mum-i fused-silica capillary (total volume, 70 nL), with a tapered tip, served as a combination of sample delivery and spraying capillary. The tapered tip of the capillary was enclosed in a subatmospheric chamber attached in front of the orifice of the mass spectrometer. The sample aspiration rate (flow rate) was regulated by computer-controlled pneumatic valves, which allowed fast switching of the pressure in the subatmospheric ESI chamber. A flow-through wash device was positioned between the microtiter plate and the ESI interface. This design allowed (en)
Title
  • Automated high-throughput infusion ESI-MS with direct coupling to a microtiter plate.
  • Automated high-throughput infusion ESI-MS with direct coupling to a microtiter plate. (en)
skos:prefLabel
  • Automated high-throughput infusion ESI-MS with direct coupling to a microtiter plate.
  • Automated high-throughput infusion ESI-MS with direct coupling to a microtiter plate. (en)
skos:notation
  • RIV/68081715:_____/01:25010018!RIV/2003/AV0/A25003/N
http://linked.open.../vavai/riv/strany
  • 1449;1454
http://linked.open...avai/riv/aktivita
http://linked.open...avai/riv/aktivity
  • Z(AV0Z4031919)
http://linked.open...iv/cisloPeriodika
  • 7
http://linked.open...vai/riv/dodaniDat
http://linked.open...aciTvurceVysledku
http://linked.open.../riv/druhVysledku
http://linked.open...iv/duvernostUdaju
http://linked.open...titaPredkladatele
http://linked.open...dnocenehoVysledku
  • 674057
http://linked.open...ai/riv/idVysledku
  • RIV/68081715:_____/01:25010018
http://linked.open...riv/jazykVysledku
http://linked.open.../riv/klicovaSlova
  • electrospray; mass-spectrometry (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...odStatuVydavatele
  • US - Spojené státy americké
http://linked.open...ontrolniKodProRIV
  • [FE1783BE85EC]
http://linked.open...i/riv/nazevZdroje
  • Analytical Chemistry
http://linked.open...in/vavai/riv/obor
http://linked.open...ichTvurcuVysledku
http://linked.open...cetTvurcuVysledku
http://linked.open...ocetUcastnikuAkce
http://linked.open...nichUcastnikuAkce
http://linked.open...UplatneniVysledku
http://linked.open...v/svazekPeriodika
  • 73
http://linked.open...iv/tvurceVysledku
  • Foret, František
  • Minarik, M.
  • Karger, B. L.
  • Goetzinger, W.
  • Felten, C.
http://linked.open...n/vavai/riv/zamer
issn
  • 0003-2700
number of pages
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