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  • Denaturing capillary electrophoresis provides much faster and more selective separations of DNA fragments than electrophoresis under native conditions. We have developed a highly alkaline electrolyte system with remarkable properties such as extraordinary denaturing power, high separation and low electroosmotic flow mobility in bare fused-silica capillaries. Thus, the analyses of a microsatellite repeat polymorphism in Endothelin 1 gene under denaturing conditions in short capillaries (2.5 cm) with laser-induced fluorescence detection were accomplished in 42 seconds.
  • Denaturing capillary electrophoresis provides much faster and more selective separations of DNA fragments than electrophoresis under native conditions. We have developed a highly alkaline electrolyte system with remarkable properties such as extraordinary denaturing power, high separation and low electroosmotic flow mobility in bare fused-silica capillaries. Thus, the analyses of a microsatellite repeat polymorphism in Endothelin 1 gene under denaturing conditions in short capillaries (2.5 cm) with laser-induced fluorescence detection were accomplished in 42 seconds. (en)
Title
  • DNA denaturing capillary electrophoresis in highly alkaline solutions.
  • DNA denaturing capillary electrophoresis in highly alkaline solutions. (en)
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  • DNA denaturing capillary electrophoresis in highly alkaline solutions.
  • DNA denaturing capillary electrophoresis in highly alkaline solutions. (en)
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  • RIV/68081715:_____/00:25200028!RIV/2003/AV0/A25003/N
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  • 37
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  • Z(AV0Z4031919)
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  • 709005
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  • RIV/68081715:_____/00:25200028
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  • N/A (en)
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  • [B0EC623472E7]
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  • Saarbrücken [DE]
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  • Saarbrücken
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  • 13th International Symposium on High Performance Capillary Electrophoresis and Related Microscale Techniques. Final Program. Book of Abstracts.
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  • Boček, Petr
  • Klepárník, Karel
  • Malá, Zdeňka
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  • Neuveden
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