About: Homology-dependent repair is involved in 45S rDNA loss in plant CAF-1 mutants

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About: Homology-dependent repair is involved in 45S rDNA loss in plant CAF-1 mutants Goto Sponge NotDistinct Permalink

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Arabidopsis thaliana mutants in FAS1 and FAS2 subunits of chromatin assembly factor 1 (CAF1) show progressive loss of 45S rDNA copies and telomeres. We hypothesized that homology-dependent DNA damage repair (HDR) may contribute to the loss of these repeats in fas mutants. To test this, we generated double mutants by crossing fas mutants with knock-out mutants in RAD51B, one of the Rad51 paralogs of A.thaliana. Our results show that the absence of RAD51B decreases the rate of rDNA loss, confirming the implication of RAD51B-dependent recombination in rDNA loss in the CAF1 mutants. Interestingly, this effect is not observed for telomeric repeat loss, which thus differs from that acting in rDNA loss. Involvement of DNA damage repair in rDNA dynamics in fas mutants is further supported by accumulation of double-stranded breaks (measured as -H2AX foci) in 45S rDNA. Occurrence of the foci is not specific for S-phase, and is ATM-independent. While the foci in fas mutants occur both in the transcribed (intranucleolar) and non-transcribed (nucleoplasmic) fraction of rDNA, double fas rad51b mutants show a specific increase in the number of the intranucleolar foci. Arabidopsis thaliana mutants in FAS1 and FAS2 subunits of chromatin assembly factor 1 (CAF1) show progressive loss of 45S rDNA copies and telomeres. We hypothesized that homology-dependent DNA damage repair (HDR) may contribute to the loss of these repeats in fas mutants. To test this, we generated double mutants by crossing fas mutants with knock-out mutants in RAD51B, one of the Rad51 paralogs of A.thaliana. Our results show that the absence of RAD51B decreases the rate of rDNA loss, confirming the implication of RAD51B-dependent recombination in rDNA loss in the CAF1 mutants. Interestingly, this effect is not observed for telomeric repeat loss, which thus differs from that acting in rDNA loss. Involvement of DNA damage repair in rDNA dynamics in fas mutants is further supported by accumulation of double-stranded breaks (measured as -H2AX foci) in 45S rDNA. Occurrence of the foci is not specific for S-phase, and is ATM-independent. While the foci in fas mutants occur both in the transcribed (intranucleolar) and non-transcribed (nucleoplasmic) fraction of rDNA, double fas rad51b mutants show a specific increase in the number of the intranucleolar foci. (en)
Title	Homology-dependent repair is involved in 45S rDNA loss in plant CAF-1 mutants Homology-dependent repair is involved in 45S rDNA loss in plant CAF-1 mutants (en)
skos:prefLabel	Homology-dependent repair is involved in 45S rDNA loss in plant CAF-1 mutants Homology-dependent repair is involved in 45S rDNA loss in plant CAF-1 mutants (en)
skos:notation	RIV/68081707:_____/15:00442213!RIV15-GA0-68081707
http://linked.open...avai/riv/aktivita	I P
http://linked.open...avai/riv/aktivity	I, P(GP13-11563P)
http://linked.open...iv/cisloPeriodika	2
http://linked.open...vai/riv/dodaniDat	2015
http://linked.open...aciTvurceVysledku	Fajkus, Jiří Dvořáčková, Martina
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Biofyzikální ústav AV ČR, v. v. i.
http://linked.open...dnocenehoVysledku	186
http://linked.open...ai/riv/idVysledku	RIV/68081707:_____/15:00442213
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	DNA repair; genome instability; 45S rDNA (en)
http://linked.open.../riv/klicoveSlovo	genome instability 45S rDNA DNA repair
http://linked.open...odStatuVydavatele	GB - Spojené království Velké Británie a Severního Irska
http://linked.open...ontrolniKodProRIV	[2E2083B75C31]
http://linked.open...i/riv/nazevZdroje	Plant Journal
http://linked.open...in/vavai/riv/obor	BO
http://linked.open...ichTvurcuVysledku	2 (xsd:int)
http://linked.open...cetTvurcuVysledku	7 (xsd:int)
http://linked.open...vavai/riv/projekt	Analysis of replication and DNA damage response in plants deficient in accurate chromatin assembly
http://linked.open...UplatneniVysledku	2015
http://linked.open...v/svazekPeriodika	81
http://linked.open...iv/tvurceVysledku	Fajkus, Jiří Mozgová, I. White, C. Dvořáčková, Martina Amiard, S. Gallego, M. E. Muchová, V.
http://linked.open...ain/vavai/riv/wos	000347830900002
issn	0960-7412
number of pages	12 (xsd:int)
http://bibframe.org/vocab/doi	10.1111/tpj.12718

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